##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccII AgeI AscI
AseI AsnI Asp718I AspI AviII
AvrII BlnI BsaI BsiEI BsiWI
BspDI BssHII BssSI BstBI BstUI
ClaI DraI EagI EclXI Eco52I
EcoRV EcoT22I FseI FspI KpnI
KspI MluI MvnI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshBI
PvuI SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AclI 4127 AACGTT AA^CGTT
AcyI 1310 GGCGCC G[GA]^CG[CT]C
Aor51HI 122 AGCGCT AGC^GCT
BbeI 1310 GGCGCC GGCGC^C
BbvCI 2318 GCTGAGG CCTCAGC(-5/-2)
BcgI 3337 GCAGACAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 2176 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1310 GGCGCC G[GA]^CG[CT]C
BspHI 472 TCATGA T^CATGA
BsrFI 5283 ACCGGC [GA]^CCGG[CT]
Cfr10I 5283 ACCGGC [GA]^CCGG[CT]
CpoI 2694 CGGTCCG CG^G[AT]CCG
Eco47III 122 AGCGCT AGC^GCT
Hin1I 1310 GGCGCC G[GA]^CG[CT]C
HpaI 4461 GTTAAC GTT^AAC
KasI 1310 GGCGCC G^GCGCC
MamI 2176 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1310 GGCGCC GG^CGCC
NdeI 5399 CATATG CA^TATG
PflMI 1860 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 4127 AACGTT AA^CGTT
RcaI 472 TCATGA T^CATGA
RsrII 2694 CGGTCCG CG^G[AT]CCG
SacI 4339 GAGCTC GAGCT^C
SfoI 1310 GGCGCC GGC^GCC
SmaI 1420 CCCGGG CCC^GGG
Van91I 1860 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2329 TCTAGA T^CTAGA
XmaCI 1420 CCCGGG C^CCGGG
XmaI 1420 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflII 3614 CTTAAG C^TTAAG
5897 CTTAAG
AflIII 5627 ACATGT A^C[GA][CT]GT
5938 ACATGT
AhdI 2864 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5758 GACTTCAAGTC
Alw44I 399 GTGCAC G^TGCAC
4608 GTGCAC
AocI 1013 CCTCAGG CC^T[ACGT]AGG
4621 CCTCAGG
ApaLI 399 GTGCAC G^TGCAC
4608 GTGCAC
Asp700I 1139 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5473 GAAGGCCTTC
AspEI 2864 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5758 GACTTCAAGTC
BamHI 948 GGATCC G^GATCC
1996 GGATCC
BanI 1310 GGCGCC G^G[CT][GA]CC
1856 GGCACC
BbrPI 29 CACGTG CAC^GTG
975 CACGTG
BfrI 3614 CTTAAG C^TTAAG
5897 CTTAAG
BglI 3059 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5378 GCCTTAATGGC
BsgI 3896 GTGCAG GTGCAG(16/14)
4003 GTGCAG
BsmI 5865 GAATGC GAATGC(1/-1)
4443 GCATTC
BspLU11I 5627 ACATGT A^CATGT
5938 ACATGT
BsrBI 167 CCGCTC CCGCTC(-3/-3)
76 GAGCGG
Bst1107I 663 GTATAC GTA^TAC
4177 GTATAC
BstAPI 779 GCAGGGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2809 GCAGTTGCTGC
BstZ17I 663 GTATAC GTA^TAC
4177 GTATAC
Bsu36I 1013 CCTCAGG CC^T[ACGT]AGG
4621 CCTCAGG
DraIII 4370 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
5203 CACTGAGTG
DrdI 241 GACACCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4619 GACCTCAGGGTC
Eam1105I 2864 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5758 GACTTCAAGTC
Eco81I 1013 CCTCAGG CC^T[ACGT]AGG
4621 CCTCAGG
HindIII 3389 AAGCTT A^AGCTT
3809 AAGCTT
MfeI 1134 CAATTG C^AATTG
2587 CAATTG
MunI 1134 CAATTG C^AATTG
2587 CAATTG
NcoI 2911 CCATGG C^CATGG
4119 CCATGG
PmaCI 29 CACGTG CAC^GTG
975 CACGTG
PmlI 29 CACGTG CAC^GTG
975 CACGTG
PshAI 1910 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2627 GACATGCGTC
PvuII 2316 CAGCTG CAG^CTG
3899 CAGCTG
ScaI 2732 AGTACT AGT^ACT
5077 AGTACT
XmnI 1139 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5473 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AccIII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BciVI BclI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaJI BsaWI
BseAI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspEI BspMI BsrDI BsrGI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PaeR7I PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoI XhoII