##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AspEI AspI AviII BbeI
BglI BsaHI BsiEI BsiWI BsmBI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
CfoI Cfr10I CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KspI MluI MspA1I MvnI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 1699 GGTACC G^GTACC
AccIII 1645 TCCGGA T^CCGGA
AlwNI 1026 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2867 CCTGAGG CC^T[ACGT]AGG
ApaI 215 GGGCCC GGGCC^C
Asp718I 1699 GGTACC G^GTACC
BamHI 275 GGATCC G^GATCC
BanI 1699 GGTACC G^G[CT][GA]CC
BbrPI 2254 CACGTG CAC^GTG
BbvCI 2985 GCTGAGG CCTCAGC(-5/-2)
BcgI 1072 GCAAATGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3723 GGATAC GTATCC(6/5)
BglII 1237 AGATCT A^GATCT
BlpI 4699 GCTGAGC GC^T[ACGT]AGC
BpmI 87 CTCCAG CTGGAG(16/14)
Bpu1102I 4699 GCTGAGC GC^T[ACGT]AGC
BsaBI 1367 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 728 GAGACC GGTCTC(1/5)
BsaWI 1645 TCCGGA [AT]^CCGG[AT]
BseAI 1645 TCCGGA T^CCGGA
BseRI 828 GAGGAG GAGGAG(10/8)
BsgI 4857 GTGCAG GTGCAG(16/14)
BspDI 4588 ATCGAT AT^CGAT
BspEI 1645 TCCGGA T^CCGGA
BstAPI 743 GCAGATAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1554 TTCGAA TT^CGAA
Bsu36I 2867 CCTGAGG CC^T[ACGT]AGG
CelII 4699 GCTGAGC GC^T[ACGT]AGC
ClaI 4588 ATCGAT AT^CGAT
Eco81I 2867 CCTGAGG CC^T[ACGT]AGG
EcoNI 4015 CCTTGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4591 GATATC GAT^ATC
EcoT22I 66 ATGCAT ATGCA^T
FauI 920 GCGGG CCCGC(4/6)
KpnI 1699 GGTACC GGTAC^C
MamI 1367 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 170 CAATTG C^AATTG
MroI 1645 TCCGGA T^CCGGA
MunI 170 CAATTG C^AATTG
NheI 2049 GCTAGC G^CTAGC
NsiI 66 ATGCAT ATGCA^T
NspV 1554 TTCGAA TT^CGAA
PflMI 14 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2254 CACGTG CAC^GTG
PmlI 2254 CACGTG CAC^GTG
PstI 3024 CTGCAG CTGCA^G
SacI 145 GAGCTC GAGCT^C
SexAI 759 ACCAGGT A^CC[AT]GGT
SfuI 1554 TTCGAA TT^CGAA
SmaI 1011 CCCGGG CCC^GGG
Tsp45I 1452 GTGAC ^GT[GC]AC
Van91I 14 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1011 CCCGGG C^CCGGG
XmaI 1011 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflII 1972 CTTAAG C^TTAAG
3728 CTTAAG
AspHI 145 GAGCTC G[AT]GC[AT]^C
3773 GAGCAC
AvaI 1011 CCCGGG C^[CT]CG[GA]G
4284 CCCGAG
AvrII 2850 CCTAGG C^CTAGG
3800 CCTAGG
BbsI 24 GAAGAC GAAGAC(2/6)
2368 GTCTTC
BclI 3168 TGATCA T^GATCA
4736 TGATCA
BcnI 1011 CCCGG CC^[GC]GG
1012 CCGGG
BfrI 1972 CTTAAG C^TTAAG
3728 CTTAAG
BlnI 2850 CCTAGG C^CTAGG
3800 CCTAGG
BpuAI 24 GAAGAC GAAGAC(2/6)
2368 GTCTTC
BsaAI 735 TACGTG [CT]AC^GT[GA]
2254 CACGTG
BsiHKAI 145 GAGCTC G[AT]GC[AT]^C
3773 GAGCAC
BsmI 3857 GAATGC GAATGC(1/-1)
3050 GCATTC
BsoBI 1011 CCCGGG C^[CT]CG[GA]G
4284 CCCGAG
BspHI 3769 TCATGA T^CATGA
4628 TCATGA
BstYI 275 GGATCC [GA]^GATC[CT]
1237 AGATCT
DraII 214 AGGGCCC [GA]G^G[ACGT]CC[CT]
215 GGGCCCC
DsaI 74 CCACGG C^C[GA][CT]GG
1161 CCACGG
EcoO109I 214 AGGGCCC [GA]G^G[ACGT]CC[CT]
215 GGGCCCC
FbaI 3168 TGATCA T^GATCA
4736 TGATCA
HapII 1012 CCGG C^CGG
1646 CCGG
HincII 304 GTCAAC GT[CT]^[GA]AC
3471 GTTGAC
HindII 304 GTCAAC GT[CT]^[GA]AC
3471 GTTGAC
HpaII 1012 CCGG C^CGG
1646 CCGG
MflI 275 GGATCC [GA]^GATC[CT]
1237 AGATCT
MslI 1560 CATTTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3865 CACAGGAATG
MspI 1012 CCGG C^CGG
1646 CCGG
NciI 1011 CCCGG CC^[GC]GG
1012 CCGGG
RcaI 3769 TCATGA T^CATGA
4628 TCATGA
StuI 2847 AGGCCT AGG^CCT
4207 AGGCCT
XhoII 275 GGATCC [GA]^GATC[CT]
1237 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AvaII BalI BanII BbvI BfaI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspLU11I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XmnI