##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AgeI Aor51HI AscI Asp718I AspI
AvaI AviII AvrII BamHI BbrPI
BbvCI BclI BlnI BsiWI BsoBI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CpoI DrdI EagI EclXI
Eco47III Eco52I EcoO65I FbaI FseI
FspI HindIII KpnI KspI MluI
NaeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmaI SpeI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccII 2914 CGCG CG^CG
AcyI 883 GGCGCC G[GA]^CG[CT]C
AflII 1572 CTTAAG C^TTAAG
AhdI 1637 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 2966 GATCC GGATC(4/5)
ApaI 4087 GGGCCC GGGCC^C
AseI 3728 ATTAAT AT^TAAT
AsnI 3728 ATTAAT AT^TAAT
AspEI 1637 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 344 TGGCCA TGG^CCA
BanI 883 GGCGCC G^G[CT][GA]CC
BbeI 883 GGCGCC GGCGC^C
BfrI 1572 CTTAAG C^TTAAG
BglI 1774 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 354 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 354 GCTGAGC GC^T[ACGT]AGC
BsaAI 4485 TACGTA [CT]AC^GT[GA]
BsaBI 4483 GATACGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 883 GGCGCC G[GA]^CG[CT]C
BsgI 448 CTGCAC GTGCAG(16/14)
BsiEI 1798 CGATCG CG[GA][CT]^CG
BsmBI 1429 CGTCTC CGTCTC(1/5)
BspDI 2836 ATCGAT AT^CGAT
BsrFI 1820 GCCGGT [GA]^CCGG[CT]
BstUI 2914 CGCG CG^CG
CelII 354 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1820 GCCGGT [GA]^CCGG[CT]
ClaI 2836 ATCGAT AT^CGAT
EaeI 344 TGGCCA [CT]^GGCC[GA]
Eam1105I 1637 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 887 CCTTACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1320 GATATC GAT^ATC
HaeII 883 GGCGCC [GA]GCGC^[CT]
Hin1I 883 GGCGCC G[GA]^CG[CT]C
HpaI 2489 GTTAAC GTT^AAC
KasI 883 GGCGCC G^GCGCC
MaeII 4486 ACGT A^CGT
MamI 4483 GATACGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 975 CAATTG C^AATTG
MluNI 344 TGGCCA TGG^CCA
MscI 344 TGGCCA TGG^CCA
MunI 975 CAATTG C^AATTG
MvnI 2914 CGCG CG^CG
NarI 883 GGCGCC GG^CGCC
NdeI 2879 CATATG CA^TATG
PflMI 226 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3728 ATTAAT AT^TAAT
PvuI 1798 CGATCG CGAT^CG
SacI 1914 GAGCTC GAGCT^C
SfoI 883 GGCGCC GGC^GCC
SnaBI 4485 TACGTA TAC^GTA
StuI 3070 AGGCCT AGG^CCT
Van91I 226 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccIII 1814 TCCGGA T^CCGGA
2899 TCCGGA
Asp700I 2288 GAATAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3453 GAAGCCATTC
BcgI 1100 CGATGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1340 GCAGTACTCTCG
BciVI 629 GGATAC GTATCC(6/5)
4482 GGATAC
BsaI 586 GAGACC GGTCTC(1/5)
4094 GAGACC
BsaWI 1814 TCCGGA [AT]^CCGG[AT]
2899 TCCGGA
BseAI 1814 TCCGGA T^CCGGA
2899 TCCGGA
BspEI 1814 TCCGGA T^CCGGA
2899 TCCGGA
DraIII 4586 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4628 CACAATGTG
EcoT22I 4605 ATGCAT ATGCA^T
4609 ATGCAT
FauI 380 GCGGG CCCGC(4/6)
3248 GCGGG
HgaI 3062 GACGC GACGC(5/10)
2044 GCGTC
HincII 191 GTTGAC GT[CT]^[GA]AC
2489 GTTAAC
HindII 191 GTTGAC GT[CT]^[GA]AC
2489 GTTAAC
MroI 1814 TCCGGA T^CCGGA
2899 TCCGGA
MslI 3303 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3856 CACAACAATG
NsiI 4605 ATGCAT ATGCA^T
4609 ATGCAT
PpuMI 310 AGGACCT [GA]G^G[AT]CC[CT]
3485 AGGACCT
SphI 2585 GCATGC GCATG^C
4607 GCATGC
XcmI 2998 CCATGCGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3275 CCACTATGCAGATGG
XmnI 2288 GAATAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3453 GAAGCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwNI AocI ApaLI ApoI AspHI
AvaII BanII BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII