##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AccII AccIII AclI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AspEI AvaI AviII AvrII BamHI
BbeI BbrPI BcgI BcnI BglI
BlnI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI BsmBI BsoBI BspEI
BspMI BssHII BstAPI BstBI BstUI
BstXI Bsu36I CelII CfoI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI FauI
FseI FspI HaeII HhaI HinP1I
KasI KspI MamI MluI MroI
MvnI NaeI NarI NciI NgoMIV
NotI NruI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SnaBI SphI Sse8387I
SwaI Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 973 GACGTC GACGT^C
Acc65I 236 GGTACC G^GTACC
AccI 95 GTATAC GT^[AC][GT]AC
AcyI 973 GACGTC G[GA]^CG[CT]C
AflII 3807 CTTAAG C^TTAAG
AflIII 4419 ACATGT A^C[GA][CT]GT
AgeI 3864 ACCGGT A^CCGGT
AseI 1326 ATTAAT AT^TAAT
AsnI 1326 ATTAAT AT^TAAT
Asp718I 236 GGTACC G^GTACC
AspI 970 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 3771 GGTCC G^G[AT]CC
BbsI 3906 GAAGAC GAAGAC(2/6)
BbvCI 3279 GCTGAGG CCTCAGC(-5/-2)
BfrI 3807 CTTAAG C^TTAAG
BpuAI 3906 GAAGAC GAAGAC(2/6)
BsaAI 3187 CACGTA [CT]AC^GT[GA]
BsaHI 973 GACGTC G[GA]^CG[CT]C
BsaI 4737 GGTCTC GGTCTC(1/5)
BsmI 2282 GCATTC GAATGC(1/-1)
BspDI 15 ATCGAT AT^CGAT
BspLU11I 4419 ACATGT A^CATGT
BsrBI 4102 GAGCGG CCGCTC(-3/-3)
BsrDI 879 GCAATG GCAATG(2/0)
BsrFI 3864 ACCGGT [GA]^CCGG[CT]
BssSI 3973 CTCGTG CACGAG(-5/-1)
Bst1107I 95 GTATAC GTA^TAC
BstEII 3110 GGTCACC G^GT[ACGT]ACC
BstPI 3110 GGTCACC G^GT[ACGT]ACC
BstZ17I 95 GTATAC GTA^TAC
Cfr10I 3864 ACCGGT [GA]^CCGG[CT]
Cfr13I 3771 GGTCC G^G[ACGT]CC
ClaI 15 ATCGAT AT^CGAT
DraII 3770 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 3770 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3110 GGTCACC G^GT[ACGT]ACC
EcoRV 3182 GATATC GAT^ATC
EcoT22I 1404 ATGCAT ATGCA^T
HgaI 2231 GCGTC GACGC(5/10)
Hin1I 973 GACGTC G[GA]^CG[CT]C
HpaI 927 GTTAAC GTT^AAC
KpnI 236 GGTACC GGTAC^C
MfeI 1909 CAATTG C^AATTG
MunI 1909 CAATTG C^AATTG
NheI 4140 GCTAGC G^CTAGC
NsiI 1404 ATGCAT ATGCA^T
PflFI 970 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3864 ACCGGT A^CCGGT
PpuMI 3770 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1326 ATTAAT AT^TAAT
SacI 4045 GAGCTC GAGCT^C
SapI 1272 GCTCTTC GCTCTTC(1/4)
Sau96I 3771 GGTCC G^G[ACGT]CC
SgrAI 3863 CACCGGTG C[GA]^CCGG[CT]G
SpeI 3815 ACTAGT A^CTAGT
StuI 1949 AGGCCT AGG^CCT
Tth111I 970 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 223 CCACAACTGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 1270 GTGCTC G[AT]GC[AT]^C
4045 GAGCTC
BalI 526 TGGCCA TGG^CCA
1413 TGGCCA
BanI 236 GGTACC G^G[CT][GA]CC
3525 GGTGCC
BanII 2450 GGGCTC G[GA]GC[CT]^C
4045 GAGCTC
BclI 464 TGATCA T^GATCA
866 TGATCA
BpmI 362 CTGGAG CTGGAG(16/14)
4120 CTGGAG
BseRI 4204 CTCCTC GAGGAG(10/8)
4057 GAGGAG
BsiHKAI 1270 GTGCTC G[AT]GC[AT]^C
4045 GAGCTC
BsmAI 4176 GAGAC GTCTC(1/5)
4738 GTCTC
Bsp1407I 979 TGTACA T^GTACA
3542 TGTACA
BspHI 2312 TCATGA T^CATGA
3157 TCATGA
BsrGI 979 TGTACA T^GTACA
3542 TGTACA
Cac8I 3563 GCTTGC GC[ACGT]^[ACGT]GC
4140 GCTAGC
FbaI 464 TGATCA T^GATCA
866 TGATCA
HincII 927 GTTAAC GT[CT]^[GA]AC
2697 GTCAAC
HindII 927 GTTAAC GT[CT]^[GA]AC
2697 GTCAAC
HindIII 3328 AAGCTT A^AGCTT
3561 AAGCTT
MluNI 526 TGGCCA TGG^CCA
1413 TGGCCA
MscI 526 TGGCCA TGG^CCA
1413 TGGCCA
PstI 2934 CTGCAG CTGCA^G
3166 CTGCAG
RcaI 2312 TCATGA T^CATGA
3157 TCATGA
SspBI 979 TGTACA T^GTACA
3542 TGTACA
SspI 1329 AATATT AAT^ATT
3391 AATATT
XbaI 2234 TCTAGA T^CTAGA
2454 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I BbvI BciVI
BfaI BglII BmyI BsaJI BsaWI
BsgI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
DdeI DpnII DraI DsaI EaeI
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI