##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
AocI Aor51HI ApaI Asp700I Asp718I
AviII BamHI BbrPI BfrI BglI
BsiWI BspDI BspHI BsrDI Bst1107I
BstZ17I Bsu36I ClaI CpoI DraI
DraIII Eco47III Eco81I FseI FspI
HincII HindII HindIII HpaI KpnI
MfeI MluI MunI NaeI NgoMIV
NheI NotI PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PvuI
PvuII RcaI RsrII SalI SbfI
SfiI SgrAI SpeI Sse8387I SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
AclI 1393 AACGTT AA^CGTT
AflIII 3283 ACATGT A^C[GA][CT]GT
AhdI 720 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 47 GGCGCGCC GG^CGCGCC
AspEI 720 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2573 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1781 CCTAGG C^CTAGG
BalI 1436 TGGCCA TGG^CCA
BanI 176 GGCGCC G^G[CT][GA]CC
BbeI 176 GGCGCC GGCGC^C
BbvCI 1506 GCTGAGG CCTCAGC(-5/-2)
BcgI 3001 CGAAGGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1896 GGATAC GTATCC(6/5)
BclI 1477 TGATCA T^GATCA
BlnI 1781 CCTAGG C^CTAGG
BsaAI 545 TACGTA [CT]AC^GT[GA]
BsaBI 981 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2592 GAGACG CGTCTC(1/5)
Bsp1407I 4297 TGTACA T^GTACA
BspLU11I 3283 ACATGT A^CATGT
BsrFI 1060 ACCGGC [GA]^CCGG[CT]
BsrGI 4297 TGTACA T^GTACA
BssSI 1581 CACGAG CACGAG(-5/-1)
BstBI 610 TTCGAA TT^CGAA
BstEII 3750 GGTAACC G^GT[ACGT]ACC
BstPI 3750 GGTAACC G^GT[ACGT]ACC
BstXI 3295 CCAAAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1060 ACCGGC [GA]^CCGG[CT]
DraII 214 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 2435 GACTAAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1140 CGGCCG C^GGCCG
Eam1105I 720 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1140 CGGCCG C^GGCCG
Eco52I 1140 CGGCCG C^GGCCG
EcoO109I 214 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3750 GGTAACC G^GT[ACGT]ACC
EcoRI 2514 GAATTC G^AATTC
EcoRV 681 GATATC GAT^ATC
FbaI 1477 TGATCA T^GATCA
KasI 176 GGCGCC G^GCGCC
KspI 43 CCGCGG CCGC^GG
MamI 981 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1436 TGGCCA TGG^CCA
MscI 1436 TGGCCA TGG^CCA
NarI 176 GGCGCC GG^CGCC
NcoI 4395 CCATGG C^CATGG
NdeI 3043 CATATG CA^TATG
NruI 1055 TCGCGA TCG^CGA
NspV 610 TTCGAA TT^CGAA
PflFI 2573 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1611 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1393 AACGTT AA^CGTT
SacII 43 CCGCGG CCGC^GG
SapI 1336 GAAGAGC GCTCTTC(1/4)
ScaI 3997 AGTACT AGT^ACT
SexAI 687 ACCAGGT A^CC[AT]GGT
SfoI 176 GGCGCC GGC^GCC
SfuI 610 TTCGAA TT^CGAA
SmaI 53 CCCGGG CCC^GGG
SnaBI 545 TACGTA TAC^GTA
SphI 412 GCATGC GCATG^C
SspBI 4297 TGTACA T^GTACA
SspI 4118 AATATT AAT^ATT
StuI 1773 AGGCCT AGG^CCT
Tth111I 2573 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3406 CCAGAGCCTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 53 CCCGGG C^CCGGG
XmaI 53 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 729 TCCGGA T^CCGGA
1493 TCCGGA
AcyI 176 GGCGCC G[GA]^CG[CT]C
917 GACGCC
AseI 3494 ATTAAT AT^TAAT
4014 ATTAAT
AsnI 3494 ATTAAT AT^TAAT
4014 ATTAAT
BglII 3074 AGATCT A^GATCT
3516 AGATCT
BlpI 1725 GCTGAGC GC^T[ACGT]AGC
2621 GCTTAGC
Bpu1102I 1725 GCTGAGC GC^T[ACGT]AGC
2621 GCTTAGC
BsaHI 176 GGCGCC G[GA]^CG[CT]C
917 GACGCC
BseAI 729 TCCGGA T^CCGGA
1493 TCCGGA
BsmI 3377 GAATGC GAATGC(1/-1)
2739 GCATTC
BspEI 729 TCCGGA T^CCGGA
1493 TCCGGA
BstAPI 412 GCATGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2609 GCACCACCTGC
CelII 1725 GCTGAGC GC^T[ACGT]AGC
2621 GCTTAGC
EcoNI 1776 CCTTACCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2825 CCTGGAACAGG
EcoT22I 936 ATGCAT ATGCA^T
3564 ATGCAT
HgaI 16 GACGC GACGC(5/10)
917 GACGC
Hin1I 176 GGCGCC G[GA]^CG[CT]C
917 GACGCC
MroI 729 TCCGGA T^CCGGA
1493 TCCGGA
NsiI 936 ATGCAT ATGCA^T
3564 ATGCAT
NspI 412 GCATGC [GA]CATG^[CT]
3283 ACATGT
PflMI 3236 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3330 CCAGAAGATGG
PshBI 3494 ATTAAT AT^TAAT
4014 ATTAAT
PstI 238 CTGCAG CTGCA^G
2725 CTGCAG
Van91I 3236 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3330 CCAGAAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII