##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AflII AgeI AhdI Alw44I
Aor51HI ApaLI Asp718I AspEI AviII
AvrII BcgI BciVI BfrI BlnI
BsiWI BspDI BsrDI BstBI BstEII
BstPI ClaI DraIII Eam1105I Eco47III
EcoO65I EcoT22I FseI FspI HindIII
KpnI NcoI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PvuI SbfI ScaI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 2385 GACGTC GACGT^C
AccIII 1270 TCCGGA T^CCGGA
AclI 3936 AACGTT AA^CGTT
AlwNI 2817 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1046 CCTGAGG CC^T[ACGT]AGG
AscI 511 GGCGCGCC GG^CGCGCC
AseI 3851 ATTAAT AT^TAAT
AsnI 3851 ATTAAT AT^TAAT
BbrPI 2579 CACGTG CAC^GTG
BbvCI 423 GCTGAGG CCTCAGC(-5/-2)
BsaBI 2748 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1270 TCCGGA [AT]^CCGG[AT]
BseAI 1270 TCCGGA T^CCGGA
BsiEI 506 CGGCCG CG[GA][CT]^CG
BsmI 3528 GAATGC GAATGC(1/-1)
BspEI 1270 TCCGGA T^CCGGA
BspLU11I 2128 ACATGT A^CATGT
Bst1107I 2986 GTATAC GTA^TAC
BstAPI 2291 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2986 GTATAC GTA^TAC
Bsu36I 1046 CCTGAGG CC^T[ACGT]AGG
CpoI 639 CGGTCCG CG^G[AT]CCG
EagI 506 CGGCCG C^GGCCG
EclXI 506 CGGCCG C^GGCCG
Eco52I 506 CGGCCG C^GGCCG
Eco81I 1046 CCTGAGG CC^T[ACGT]AGG
EcoRI 2409 GAATTC G^AATTC
EcoRV 1463 GATATC GAT^ATC
HpaI 3278 GTTAAC GTT^AAC
MamI 2748 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3327 CAATTG C^AATTG
MluI 1903 ACGCGT A^CGCGT
MroI 1270 TCCGGA T^CCGGA
MunI 3327 CAATTG C^AATTG
NheI 220 GCTAGC G^CTAGC
PmaCI 2579 CACGTG CAC^GTG
PmlI 2579 CACGTG CAC^GTG
PpuMI 1549 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 3851 ATTAAT AT^TAAT
Psp1406I 3936 AACGTT AA^CGTT
PstI 786 CTGCAG CTGCA^G
RsrII 639 CGGTCCG CG^G[AT]CCG
SalI 2388 GTCGAC G^TCGAC
SapI 2372 GCTCTTC GCTCTTC(1/4)
SgrAI 294 CGCCGGCG C[GA]^CCGG[CT]G
StuI 1091 AGGCCT AGG^CCT
Tsp45I 2518 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 2388 GTCGAC GT^[AC][GT]AC
2986 GTATAC
ApaI 342 GGGCCC GGGCC^C
605 GGGCCC
Asp700I 935 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2409 GAATTCCTTC
BamHI 1998 GGATCC G^GATCC
2343 GGATCC
BglII 1364 AGATCT A^GATCT
2597 AGATCT
BsaI 1027 GAGACC GGTCTC(1/5)
3178 GAGACC
BsmBI 3497 GAGACG CGTCTC(1/5)
3665 GAGACG
BssSI 2193 CTCGTG CACGAG(-5/-1)
3754 CTCGTG
BstXI 3191 CCAGTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3563 CCACTTCCCTGG
DrdI 1710 GACGACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2801 GACCTATGGGTC
EcoT14I 1705 CCTTGG C^C[AT][AT]GG
2633 CCAAGG
HgaI 98 GACGC GACGC(5/10)
167 GCGTC
PflMI 968 CCACTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1934 CCAGTTTGTGG
SacI 1470 GAGCTC GAGCT^C
4080 GAGCTC
SmaI 366 CCCGGG CCC^GGG
580 CCCGGG
StyI 1705 CCTTGG C^C[AT][AT]GG
2633 CCAAGG
Van91I 968 CCACTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1934 CCAGTTTGTGG
XcmI 1102 CCACGGAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2812 CCACACAGCAGCTGG
XmaCI 366 CCCGGG C^CCGGG
580 CCCGGG
XmaI 366 CCCGGG C^CCGGG
580 CCCGGG
XmnI 935 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2409 GAATTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrBI BsrFI
BsrGI BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PleI PvuII RcaI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SspBI
SspI TaqI TfiI Tru9I TseI
Tsp509I TspRI Tth111I TthHB8I XhoII