##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AclI AflII AgeI Aor51HI
AscI Asp700I AspI AviII AvrII
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsiWI BsmBI Bsp1407I BspDI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI Eco47III EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI Psp1406I PvuI RsrII
SalI ScaI SfiI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
TfiI Tth111I XbaI XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 1801 GGTACC G^GTACC
AccI 1998 GTAGAC GT^[AC][GT]AC
AccIII 1301 TCCGGA T^CCGGA
AseI 3067 ATTAAT AT^TAAT
AsnI 3067 ATTAAT AT^TAAT
Asp718I 1801 GGTACC G^GTACC
BbrPI 2911 CACGTG CAC^GTG
BcgI 1901 GCATCCACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 325 AGATCT A^GATCT
BsaBI 693 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 271 GGTCTC GGTCTC(1/5)
BseAI 1301 TCCGGA T^CCGGA
BsiEI 539 CGGCCG CG[GA][CT]^CG
BsmI 2460 GAATGC GAATGC(1/-1)
BspEI 1301 TCCGGA T^CCGGA
BspHI 1376 TCATGA T^CATGA
BsrBI 1158 GAGCGG CCGCTC(-3/-3)
DrdI 1453 GACACCCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 539 CGGCCG C^GGCCG
EclXI 539 CGGCCG C^GGCCG
Eco52I 539 CGGCCG C^GGCCG
EcoRI 867 GAATTC G^AATTC
KpnI 1801 GGTACC GGTAC^C
KspI 2182 CCGCGG CCGC^GG
MamI 693 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1301 TCCGGA T^CCGGA
NdeI 159 CATATG CA^TATG
PmaCI 2911 CACGTG CAC^GTG
PmlI 2911 CACGTG CAC^GTG
PshAI 3355 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3067 ATTAAT AT^TAAT
RcaI 1376 TCATGA T^CATGA
SacI 520 GAGCTC GAGCT^C
SacII 2182 CCGCGG CCGC^GG
SbfI 1410 CCTGCAGG CCTGCA^GG
SphI 186 GCATGC GCATG^C
Sse8387I 1410 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 1459 CGCG CG^CG
2183 CGCG
AflIII 1652 ACGTGT A^C[GA][CT]GT
2912 ACGTGT
Alw44I 3247 GTGCAC G^TGCAC
3432 GTGCAC
AocI 1503 CCTCAGG CC^T[ACGT]AGG
2541 CCTAAGG
ApaLI 3247 GTGCAC G^TGCAC
3432 GTGCAC
BamHI 1775 GGATCC G^GATCC
2023 GGATCC
BbsI 1788 GAAGAC GAAGAC(2/6)
2695 GTCTTC
BfaI 3028 CTAG C^TAG
3220 CTAG
BpuAI 1788 GAAGAC GAAGAC(2/6)
2695 GTCTTC
BsaWI 725 ACCGGA [AT]^CCGG[AT]
1301 TCCGGA
BssSI 1750 CACGAG CACGAG(-5/-1)
3314 CACGAG
BstAPI 2621 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2748 GCAGGTGCTGC
BstUI 1459 CGCG CG^CG
2183 CGCG
Bsu36I 1503 CCTCAGG CC^T[ACGT]AGG
2541 CCTAAGG
Eco81I 1503 CCTCAGG CC^T[ACGT]AGG
2541 CCTAAGG
MaeI 3028 CTAG C^TAG
3220 CTAG
MseI 1180 TTAA T^TAA
3068 TTAA
MvnI 1459 CGCG CG^CG
2183 CGCG
SapI 2295 GAAGAGC GCTCTTC(1/4)
3264 GAAGAGC
SmaI 280 CCCGGG CCC^GGG
2019 CCCGGG
Tru9I 1180 TTAA T^TAA
3068 TTAA
XmaCI 280 CCCGGG C^CCGGG
2019 CCCGGG
XmaI 280 CCCGGG C^CCGGG
2019 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AcyI AfaI AhdI
AluI AlwI AlwNI ApaI ApoI
AspEI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BcnI BglI BmyI BpmI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI Eam1105I
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmlI StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII