##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AclI AgeI AhdI AlwI
AlwNI AscI AspEI AspI AviII
AvrII BamHI BbeI BciVI BglI
BlnI BsaBI BspDI BspHI BssSI
BstAPI BstBI BstXI ClaI Eam1105I
EcoRI EcoRV FseI FspI HindIII
KasI MamI MluI NarI NcoI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI Psp1406I
PvuI RcaI SapI SbfI SexAI
SfiI SfoI SfuI SpeI Sse8387I
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 1910 GGTACC G^GTACC
AccIII 84 TCCGGA T^CCGGA
AflII 3600 CTTAAG C^TTAAG
Alw44I 1735 GTGCAC G^TGCAC
Aor51HI 730 AGCGCT AGC^GCT
ApaI 397 GGGCCC GGGCC^C
ApaLI 1735 GTGCAC G^TGCAC
AseI 4012 ATTAAT AT^TAAT
AsnI 4012 ATTAAT AT^TAAT
Asp718I 1910 GGTACC G^GTACC
BalI 930 TGGCCA TGG^CCA
BbrPI 1766 CACGTG CAC^GTG
BbsI 583 GTCTTC GAAGAC(2/6)
BcgI 2212 GCAGCACTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3600 CTTAAG C^TTAAG
BlpI 523 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 523 GCTGAGC GC^T[ACGT]AGC
BpuAI 583 GTCTTC GAAGAC(2/6)
BsaWI 84 TCCGGA [AT]^CCGG[AT]
BseAI 84 TCCGGA T^CCGGA
BsgI 273 CTGCAC GTGCAG(16/14)
BsiWI 1689 CGTACG C^GTACG
BsmBI 1532 GAGACG CGTCTC(1/5)
Bsp1407I 3960 TGTACA T^GTACA
BspEI 84 TCCGGA T^CCGGA
BsrGI 3960 TGTACA T^GTACA
Bst1107I 2082 GTATAC GTA^TAC
BstEII 1646 GGTTACC G^GT[ACGT]ACC
BstPI 1646 GGTTACC G^GT[ACGT]ACC
BstZ17I 2082 GTATAC GTA^TAC
CelII 523 GCTGAGC GC^T[ACGT]AGC
CpoI 51 CGGTCCG CG^G[AT]CCG
DrdI 1630 GACTGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 730 AGCGCT AGC^GCT
EcoNI 3599 CCTTAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1646 GGTTACC G^GT[ACGT]ACC
HpaI 942 GTTAAC GTT^AAC
KpnI 1910 GGTACC GGTAC^C
MfeI 2603 CAATTG C^AATTG
MluNI 930 TGGCCA TGG^CCA
MroI 84 TCCGGA T^CCGGA
MscI 930 TGGCCA TGG^CCA
MunI 2603 CAATTG C^AATTG
NdeI 3663 CATATG CA^TATG
PflMI 3543 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1766 CACGTG CAC^GTG
PmlI 1766 CACGTG CAC^GTG
PshBI 4012 ATTAAT AT^TAAT
RsrII 51 CGGTCCG CG^G[AT]CCG
SacI 262 GAGCTC GAGCT^C
SalI 968 GTCGAC G^TCGAC
SgrAI 755 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 657 CCCGGG CCC^GGG
SnaBI 3891 TACGTA TAC^GTA
SphI 2012 GCATGC GCATG^C
SspBI 3960 TGTACA T^GTACA
StuI 1504 AGGCCT AGG^CCT
SwaI 3009 ATTTAAAT ATTT^AAAT
Van91I 3543 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1513 TCTAGA T^CTAGA
XmaCI 657 CCCGGG C^CCGGG
XmaI 657 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Asp700I 1543 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1815 GAACTCATTC
BbvCI 726 CCTCAGC CCTCAGC(-5/-2)
2195 CCTCAGC
BsaAI 1766 CACGTG [CT]AC^GT[GA]
3891 TACGTA
BsaI 1100 GGTCTC GGTCTC(1/5)
1475 GGTCTC
BsiEI 483 CGGCCG CG[GA][CT]^CG
761 CGGCCG
BspMI 37 ACCTGC ACCTGC(4/8)
1336 ACCTGC
DraIII 2145 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4027 CACCGGGTG
EagI 483 CGGCCG C^GGCCG
761 CGGCCG
EarI 1018 GAAGAG CTCTTC(1/4)
2796 GAAGAG
EclXI 483 CGGCCG C^GGCCG
761 CGGCCG
Eco52I 483 CGGCCG C^GGCCG
761 CGGCCG
EcoT22I 3660 ATGCAT ATGCA^T
4083 ATGCAT
Ksp632I 1018 GAAGAG CTCTTC(1/4)
2796 GAAGAG
KspI 55 CCGCGG CCGC^GG
716 CCGCGG
NsiI 3660 ATGCAT ATGCA^T
4083 ATGCAT
PleI 1067 GACTC GAGTC(4/5)
4 GAGTC
PshAI 662 GACAGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4154 GACTTATGTC
PstI 67 CTGCAG CTGCA^G
1620 CTGCAG
PvuII 1053 CAGCTG CAG^CTG
3306 CAGCTG
SacII 55 CCGCGG CCGC^GG
716 CCGCGG
ScaI 2719 AGTACT AGT^ACT
4268 AGTACT
SmlI 3600 CTTAAG C^T[CT][GA]AG
4021 CTTGAG
XmnI 1543 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1815 GAACTCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AocI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BclI BcnI BfaI BglII
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BsrBI
BsrDI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII