##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
Aor51HI ApaLI AscI AspI AviII
BbeI BglI BsaBI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I FseI FspI KasI KspI
MamI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmeI Psp1406I
PvuI RcaI RsrII SacII SfiI
SfoI SfuI SgrAI SnaBI SphI
Tth111I
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 4239 GGTACC G^GTACC
AhdI 3638 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3980 CCTAAGG CC^T[ACGT]AGG
Asp700I 5523 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4239 GGTACC G^GTACC
AspEI 3638 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4894 CCTAGG C^CTAGG
BclI 4435 TGATCA T^GATCA
BglII 3087 AGATCT A^GATCT
BlnI 4894 CCTAGG C^CTAGG
BsaWI 2556 ACCGGA [AT]^CCGG[AT]
BsrBI 1151 GAGCGG CCGCTC(-3/-3)
BstAPI 7050 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3980 CCTAAGG CC^T[ACGT]AGG
DsaI 4696 CCATGG C^C[GA][CT]GG
Eam1105I 3638 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3980 CCTAAGG CC^T[ACGT]AGG
FbaI 4435 TGATCA T^GATCA
HaeII 886 AGCGCC [GA]GCGC^[CT]
HpaI 943 GTTAAC GTT^AAC
KpnI 4239 GGTACC GGTAC^C
MfeI 2952 CAATTG C^AATTG
MunI 2952 CAATTG C^AATTG
NcoI 4696 CCATGG C^CATGG
NdeI 1920 CATATG CA^TATG
PaeR7I 1719 CTCGAG C^TCGAG
SacI 3597 GAGCTC GAGCT^C
SalI 3030 GTCGAC G^TCGAC
SbfI 1221 CCTGCAGG CCTGCA^GG
ScaI 1407 AGTACT AGT^ACT
SexAI 204 ACCAGGT A^CC[AT]GGT
SmaI 4023 CCCGGG CCC^GGG
SpeI 5916 ACTAGT A^CTAGT
Sse8387I 1221 CCTGCAGG CCTGCA^GG
XhoI 1719 CTCGAG C^TCGAG
XmaCI 4023 CCCGGG C^CCGGG
XmaI 4023 CCCGGG C^CCGGG
XmnI 5523 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaI 1719 CTCGAG C^[CT]CG[GA]G
4023 CCCGGG
BalI 2536 TGGCCA TGG^CCA
7699 TGGCCA
BbrPI 779 CACGTG CAC^GTG
4781 CACGTG
BcgI 4508 CGACACCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4901 GCAACACTTTCG
BciVI 5923 GGATAC GTATCC(6/5)
2266 GTATCC
BsaI 4692 GAGACC GGTCTC(1/5)
6421 GGTCTC
BsoBI 1719 CTCGAG C^[CT]CG[GA]G
4023 CCCGGG
BspMI 1366 ACCTGC ACCTGC(4/8)
3482 ACCTGC
BssSI 2116 CACGAG CACGAG(-5/-1)
2751 CACGAG
BstXI 3218 CCACCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7418 CCATCCGTCTGG
EaeI 2536 TGGCCA [CT]^GGCC[GA]
7699 TGGCCA
MluNI 2536 TGGCCA TGG^CCA
7699 TGGCCA
MscI 2536 TGGCCA TGG^CCA
7699 TGGCCA
PmaCI 779 CACGTG CAC^GTG
4781 CACGTG
PmlI 779 CACGTG CAC^GTG
4781 CACGTG
PshAI 4086 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4694 GACCATGGTC
SapI 1380 GAAGAGC GCTCTTC(1/4)
4534 GCTCTTC
SwaI 6471 ATTTAAAT ATTT^AAAT
7511 ATTTAAAT
XcmI 3354 CCAACCCCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
7021 CCAAAACCCTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI AlwI
AlwNI ApaI ApoI AseI AsnI
AspHI AvaII BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BfrI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII EarI EcoNI EcoO109I
EcoRI EcoRII EcoRV EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII