##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I AviII AvrII BbeI
BclI BfrI BlnI BlpI Bpu1102I
BsaBI BsaHI BsiWI BsmBI BspDI
BsrDI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI DrdI EcoRI
EcoRV FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
SalI SbfI ScaI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AhdI 3941 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3941 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1107 GGATCC G^GATCC
BbsI 3635 GTCTTC GAAGAC(2/6)
BbvCI 236 GCTGAGG CCTCAGC(-5/-2)
BciVI 653 GGATAC GTATCC(6/5)
BpuAI 3635 GTCTTC GAAGAC(2/6)
Bsp1407I 669 TGTACA T^GTACA
BspHI 957 TCATGA T^CATGA
BspLU11I 603 ACATGT A^CATGT
BsrFI 756 GCCGGC [GA]^CCGG[CT]
BsrGI 669 TGTACA T^GTACA
BstXI 510 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 756 GCCGGC [GA]^CCGG[CT]
CpoI 77 CGGACCG CG^G[AT]CCG
EagI 22 CGGCCG C^GGCCG
Eam1105I 3941 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 22 CGGCCG C^GGCCG
Eco52I 22 CGGCCG C^GGCCG
EcoT22I 2773 ATGCAT ATGCA^T
HgaI 431 GACGC GACGC(5/10)
HincII 347 GTCAAC GT[CT]^[GA]AC
HindII 347 GTCAAC GT[CT]^[GA]AC
KspI 798 CCGCGG CCGC^GG
NaeI 756 GCCGGC GCC^GGC
NgoMIV 756 GCCGGC G^CCGGC
NsiI 2773 ATGCAT ATGCA^T
PshAI 142 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1099 CGATCG CGAT^CG
PvuII 3100 CAGCTG CAG^CTG
RcaI 957 TCATGA T^CATGA
RsrII 77 CGGACCG CG^G[AT]CCG
SacII 798 CCGCGG CCGC^GG
SexAI 3451 ACCTGGT A^CC[AT]GGT
SfiI 491 GGCCCCGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2771 GCATGC GCATG^C
SspBI 669 TGTACA T^GTACA
XcmI 3106 CCAACTTAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 939 TCCGGA T^CCGGA
3034 TCCGGA
AocI 2545 CCTCAGG CC^T[ACGT]AGG
2655 CCTGAGG
Aor51HI 264 AGCGCT AGC^GCT
1698 AGCGCT
BglI 492 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1870 GCCATTCGGGC
BsaI 2164 GAGACC GGTCTC(1/5)
859 GGTCTC
BsaWI 939 TCCGGA [AT]^CCGG[AT]
3034 TCCGGA
BseAI 939 TCCGGA T^CCGGA
3034 TCCGGA
BspEI 939 TCCGGA T^CCGGA
3034 TCCGGA
BstEII 298 GGTCACC G^GT[ACGT]ACC
340 GGTCACC
BstPI 298 GGTCACC G^GT[ACGT]ACC
340 GGTCACC
Bsu36I 2545 CCTCAGG CC^T[ACGT]AGG
2655 CCTGAGG
DraI 3504 TTTAAA TTT^AAA
3641 TTTAAA
Eco47III 264 AGCGCT AGC^GCT
1698 AGCGCT
Eco81I 2545 CCTCAGG CC^T[ACGT]AGG
2655 CCTGAGG
EcoO65I 298 GGTCACC G^GT[ACGT]ACC
340 GGTCACC
MroI 939 TCCGGA T^CCGGA
3034 TCCGGA
NcoI 1588 CCATGG C^CATGG
2471 CCATGG
PflMI 745 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1226 CCAGATTTTGG
SmaI 1245 CCCGGG CCC^GGG
2493 CCCGGG
SmlI 596 CTCAAG C^T[CT][GA]AG
1727 CTCAAG
Van91I 745 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1226 CCAGATTTTGG
XmaCI 1245 CCCGGG C^CCGGG
2493 CCCGGG
XmaI 1245 CCCGGG C^CCGGG
2493 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI Asp700I AspHI AspI AvaI
AvaII BalI BanI BanII BbrPI
BbvI BcgI BcnI BfaI BglII
BmyI BpmI BsaAI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PleI PmaCI
PmlI PpuMI PstI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII XmnI