##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccI AclI AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp700I AspEI AviII AvrII
BbrPI BcgI BglI BlnI BsaAI
BsaBI BsiWI Bsp1407I BspDI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoT22I FseI
FspI HpaI MamI MfeI MluI
MunI NcoI NdeI NotI NruI
NsiI NspV PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Van91I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2207 GGTACC G^GTACC
AccIII 336 TCCGGA T^CCGGA
AseI 4062 ATTAAT AT^TAAT
AsnI 4062 ATTAAT AT^TAAT
Asp718I 2207 GGTACC G^GTACC
AspI 4400 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4230 TGGCCA TGG^CCA
BamHI 1415 GGATCC G^GATCC
BbsI 2243 GAAGAC GAAGAC(2/6)
BbvCI 4323 GCTGAGG CCTCAGC(-5/-2)
BciVI 3100 GTATCC GTATCC(6/5)
BglII 2608 AGATCT A^GATCT
BlpI 494 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 494 GCTCAGC GC^T[ACGT]AGC
BpuAI 2243 GAAGAC GAAGAC(2/6)
BsaWI 336 TCCGGA [AT]^CCGG[AT]
BseAI 336 TCCGGA T^CCGGA
BsmI 3994 GCATTC GAATGC(1/-1)
BspEI 336 TCCGGA T^CCGGA
BspHI 2236 TCATGA T^CATGA
BsrBI 228 CCGCTC CCGCTC(-3/-3)
CelII 494 GCTCAGC GC^T[ACGT]AGC
CpoI 1611 CGGACCG CG^G[AT]CCG
DraII 1848 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 2217 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1848 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 989 GATATC GAT^ATC
HindIII 3878 AAGCTT A^AGCTT
KpnI 2207 GGTACC GGTAC^C
MaeII 1366 ACGT A^CGT
MluNI 4230 TGGCCA TGG^CCA
MroI 336 TCCGGA T^CCGGA
MscI 4230 TGGCCA TGG^CCA
NspI 2540 GCATGT [GA]CATG^[CT]
PacI 4063 TTAATTAA TTAAT^TAA
PflFI 4400 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1848 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 4062 ATTAAT AT^TAAT
RcaI 2236 TCATGA T^CATGA
RsrII 1611 CGGACCG CG^G[AT]CCG
SacI 332 GAGCTC GAGCT^C
SexAI 2709 ACCAGGT A^CC[AT]GGT
Tth111I 4400 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 895 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 2351 CCTGAGG CC^T[ACGT]AGG
3712 CCTCAGG
AvaI 247 CTCGGG C^[CT]CG[GA]G
2508 CCCGAG
BanII 332 GAGCTC G[GA]GC[CT]^C
3846 GGGCTC
BclI 1198 TGATCA T^GATCA
2254 TGATCA
BsgI 546 CTGCAC GTGCAG(16/14)
1746 GTGCAG
BsmBI 4250 CGTCTC CGTCTC(1/5)
2128 GAGACG
BsoBI 247 CTCGGG C^[CT]CG[GA]G
2508 CCCGAG
BspMI 1167 ACCTGC ACCTGC(4/8)
4292 GCAGGT
BsrDI 2865 CATTGC GCAATG(2/0)
795 GCAATG
BstXI 3835 CCAATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4308 CCAGCTGCTTGG
Bsu36I 2351 CCTGAGG CC^T[ACGT]AGG
3712 CCTCAGG
DraI 3363 TTTAAA TTT^AAA
3563 TTTAAA
EagI 82 CGGCCG C^GGCCG
1280 CGGCCG
EclXI 82 CGGCCG C^GGCCG
1280 CGGCCG
Eco52I 82 CGGCCG C^GGCCG
1280 CGGCCG
Eco81I 2351 CCTGAGG CC^T[ACGT]AGG
3712 CCTCAGG
FbaI 1198 TGATCA T^GATCA
2254 TGATCA
KspI 126 CCGCGG CCGC^GG
147 CCGCGG
NheI 2150 GCTAGC G^CTAGC
3954 GCTAGC
SacII 126 CCGCGG CCGC^GG
147 CCGCGG
ScaI 1628 AGTACT AGT^ACT
3604 AGTACT
XcmI 910 CCAGATTGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1419 CCAAAGAATCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AluI AlwI AlwNI ApoI
AspHI AvaII BanI BbeI BbvI
BcnI BfaI BfrI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EarI EcoNI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII