##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Aor51HI AscI Asp718I AspEI AviII
AvrII BalI BamHI BbrPI BfrI
BlnI BsiEI BsiWI BsmBI Bsp1407I
BspDI BsrGI ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
FseI FspI HpaI KpnI MfeI
MluI MluNI MscI MunI NheI
NotI NruI PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SbfI SgrAI SnaBI
Sse8387I SspBI SwaI XbaI XcmI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 926 GACGTC GACGT^C
Alw44I 635 GTGCAC G^TGCAC
ApaLI 635 GTGCAC G^TGCAC
AspI 4345 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 101 GGCGCC GGCGC^C
BcgI 2264 GCATTCTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2482 TGATCA T^GATCA
BglII 4454 AGATCT A^GATCT
BlpI 1051 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1051 GCTGAGC GC^T[ACGT]AGC
BsaAI 2466 TACGTG [CT]AC^GT[GA]
BsaBI 3094 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 1116 TCATGA T^CATGA
BsrDI 2067 GCAATG GCAATG(2/0)
BsrFI 173 GCCGGC [GA]^CCGG[CT]
BssHII 302 GCGCGC G^CGCGC
BssSI 1877 CACGAG CACGAG(-5/-1)
Bst1107I 326 GTATAC GTA^TAC
BstAPI 1616 GCACACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 505 GGTAACC G^GT[ACGT]ACC
BstPI 505 GGTAACC G^GT[ACGT]ACC
BstZ17I 326 GTATAC GTA^TAC
CelII 1051 GCTGAGC GC^T[ACGT]AGC
Cfr10I 173 GCCGGC [GA]^CCGG[CT]
DrdI 237 GACCCTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 936 TGGCCG [CT]^GGCC[GA]
EcoNI 2389 CCTCACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 505 GGTAACC G^GT[ACGT]ACC
EcoRV 3181 GATATC GAT^ATC
EcoT22I 2354 ATGCAT ATGCA^T
FbaI 2482 TGATCA T^GATCA
KasI 101 GGCGCC G^GCGCC
MamI 3094 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 173 GCCGGC GCC^GGC
NarI 101 GGCGCC GG^CGCC
NcoI 963 CCATGG C^CATGG
NdeI 4258 CATATG CA^TATG
NgoMIV 173 GCCGGC G^CCGGC
NsiI 2354 ATGCAT ATGCA^T
PaeR7I 1965 CTCGAG C^TCGAG
PflFI 4345 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 250 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1116 TCATGA T^CATGA
SalI 108 GTCGAC G^TCGAC
SexAI 1595 ACCAGGT A^CC[AT]GGT
SfiI 958 GGCCTCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 101 GGCGCC GGC^GCC
SmaI 794 CCCGGG CCC^GGG
SpeI 1726 ACTAGT A^CTAGT
SphI 870 GCATGC GCATG^C
Tth111I 4345 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1965 CTCGAG C^TCGAG
XmaCI 794 CCCGGG C^CCGGG
XmaI 794 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 366 GATCC GGATC(4/5)
2777 GATCC
AlwNI 1397 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3501 CAGAGACTG
AseI 2453 ATTAAT AT^TAAT
4209 ATTAAT
AsnI 2453 ATTAAT AT^TAAT
4209 ATTAAT
Asp700I 2790 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3302 GAAGGACTTC
AspHI 635 GTGCAC G[AT]GC[AT]^C
3964 GAGCAC
AvaI 794 CCCGGG C^[CT]CG[GA]G
1965 CTCGAG
BciVI 2647 GGATAC GTATCC(6/5)
56 GTATCC
BglI 793 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
959 GCCTCCATGGC
BsaI 342 GAGACC GGTCTC(1/5)
3524 GGTCTC
BsiHKAI 635 GTGCAC G[AT]GC[AT]^C
3964 GAGCAC
BsoBI 794 CCCGGG C^[CT]CG[GA]G
1965 CTCGAG
BspMI 404 ACCTGC ACCTGC(4/8)
3226 ACCTGC
BstXI 1059 CCATCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3928 CCAGCTACTTGG
BstYI 2776 AGATCC [GA]^GATC[CT]
4454 AGATCT
HincII 108 GTCGAC GT[CT]^[GA]AC
4470 GTCAAC
HindII 108 GTCGAC GT[CT]^[GA]AC
4470 GTCAAC
HindIII 1467 AAGCTT A^AGCTT
2367 AAGCTT
MflI 2776 AGATCC [GA]^GATC[CT]
4454 AGATCT
PflMI 377 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3068 CCAGTCGGTGG
PshBI 2453 ATTAAT AT^TAAT
4209 ATTAAT
SapI 1580 GAAGAGC GCTCTTC(1/4)
2093 GAAGAGC
ScaI 1224 AGTACT AGT^ACT
2497 AGTACT
SfaNI 1871 GATGC GCATC(5/9)
2867 GCATC
StuI 545 AGGCCT AGG^CCT
1452 AGGCCT
Van91I 377 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3068 CCAGTCGGTGG
XhoII 2776 AGATCC [GA]^GATC[CT]
4454 AGATCT
XmnI 2790 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3302 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AluI AocI
ApaI ApoI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseAI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspEI BspLU11I BsrBI BsrI BssKI
BstBI BstF5I BstNI BstUI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MnlI MroI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI NspV
PleI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfcI SfuI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I