##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AviII BamHI BbeI BbrPI
BbvCI BcgI BcnI BglI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrGI BssSI
BstAPI BstBI BstEII BstPI BstXI
Bsu36I CelII ClaI CpoI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRV FseI FspI Hin1I
HindIII KasI KpnI MamI MluI
MroI NarI NciI NcoI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 3643 GTATAC GT^[AC][GT]AC
AflII 2038 CTTAAG C^TTAAG
Alw44I 3164 GTGCAC G^TGCAC
AlwI 2347 GATCC GGATC(4/5)
AlwNI 77 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 3164 GTGCAC G^TGCAC
Asp700I 1256 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 3164 GTGCAC G[AT]GC[AT]^C
AvaI 116 CCCGAG C^[CT]CG[GA]G
AvaII 1561 GGACC G^G[AT]CC
AvrII 3582 CCTAGG C^CTAGG
BanII 1676 GAGCCC G[GA]GC[CT]^C
BciVI 1821 GTATCC GTATCC(6/5)
BclI 2829 TGATCA T^GATCA
BfrI 2038 CTTAAG C^TTAAG
BlnI 3582 CCTAGG C^CTAGG
BpmI 535 CTCCAG CTGGAG(16/14)
BsaI 244 GGTCTC GGTCTC(1/5)
BsiEI 239 CGGCCG CG[GA][CT]^CG
BsiHKAI 3164 GTGCAC G[AT]GC[AT]^C
BsmI 3856 GCATTC GAATGC(1/-1)
BsoBI 116 CCCGAG C^[CT]CG[GA]G
BspHI 420 TCATGA T^CATGA
BssHII 133 GCGCGC G^CGCGC
Bst1107I 3643 GTATAC GTA^TAC
BstZ17I 3643 GTATAC GTA^TAC
DsaI 184 CCGCGG C^C[GA][CT]GG
EagI 239 CGGCCG C^GGCCG
EclXI 239 CGGCCG C^GGCCG
Eco52I 239 CGGCCG C^GGCCG
EcoRI 3546 GAATTC G^AATTC
EcoT14I 3582 CCTAGG C^C[AT][AT]GG
FbaI 2829 TGATCA T^GATCA
KspI 184 CCGCGG CCGC^GG
MaeII 880 ACGT A^CGT
MfeI 348 CAATTG C^AATTG
MslI 2833 CATTTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 348 CAATTG C^AATTG
NaeI 16 GCCGGC GCC^GGC
NgoMIV 16 GCCGGC G^CCGGC
PflMI 729 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1560 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 420 TCATGA T^CATGA
SacII 184 CCGCGG CCGC^GG
SapI 1358 GAAGAGC GCTCTTC(1/4)
SmlI 2038 CTTAAG C^T[CT][GA]AG
StyI 3582 CCTAGG C^C[AT][AT]GG
Van91I 729 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1256 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BalI 1879 TGGCCA TGG^CCA
2154 TGGCCA
BanI 2286 GGCACC G^G[CT][GA]CC
3714 GGTGCC
BbsI 602 GAAGAC GAAGAC(2/6)
2301 GTCTTC
BmyI 1676 GAGCCC G[AGT]GC[ACT]^C
3164 GTGCAC
BpuAI 602 GAAGAC GAAGAC(2/6)
2301 GTCTTC
BsmFI 1797 GGGAC GGGAC(10/14)
79 GTCCC
Bsp1286I 1676 GAGCCC G[AGT]GC[ACT]^C
3164 GTGCAC
BsrFI 16 GCCGGC [GA]^CCGG[CT]
241 GCCGGT
Cfr10I 16 GCCGGC [GA]^CCGG[CT]
241 GCCGGT
EcoT22I 1521 ATGCAT ATGCA^T
3854 ATGCAT
HaeII 120 AGCGCC [GA]GCGC^[CT]
127 AGCGCC
HapII 17 CCGG C^CGG
242 CCGG
HgaI 891 GACGC GACGC(5/10)
2116 GCGTC
HpaI 2865 GTTAAC GTT^AAC
3074 GTTAAC
HpaII 17 CCGG C^CGG
242 CCGG
MluNI 1879 TGGCCA TGG^CCA
2154 TGGCCA
MscI 1879 TGGCCA TGG^CCA
2154 TGGCCA
MspI 17 CCGG C^CGG
242 CCGG
NdeI 1916 CATATG CA^TATG
3188 CATATG
NsiI 1521 ATGCAT ATGCA^T
3854 ATGCAT
PstI 147 CTGCAG CTGCA^G
3653 CTGCAG
ScaI 437 AGTACT AGT^ACT
3921 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI ApoI BbvI BfaI BglII
BsaJI BseRI BsgI BsiYI BslI
BsmAI BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII