##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AspEI AvrII BbeI BbrPI
BcgI BciVI BfrI BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII BstEII BstPI BstXI
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FseI HpaI KasI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NotI NruI PacI
PinAI PmaCI PmeI PmlI PvuI
RsrII SacI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspI StuI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AclI 17 AACGTT AA^CGTT
AcyI 400 GGCGTC G[GA]^CG[CT]C
AflIII 3209 ACGTGT A^C[GA][CT]GT
AseI 4291 ATTAAT AT^TAAT
AsnI 4291 ATTAAT AT^TAAT
AspI 3099 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 164 TGCGCA TGC^GCA
BalI 3447 TGGCCA TGG^CCA
BamHI 139 GGATCC G^GATCC
BbvCI 1111 CCTCAGC CCTCAGC(-5/-2)
BlpI 1166 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1166 GCTCAGC GC^T[ACGT]AGC
BsaHI 400 GGCGTC G[GA]^CG[CT]C
BsaI 453 GGTCTC GGTCTC(1/5)
BspHI 93 TCATGA T^CATGA
BspMI 3415 GCAGGT ACCTGC(4/8)
BsrDI 1522 GCAATG GCAATG(2/0)
Bst1107I 2107 GTATAC GTA^TAC
BstAPI 1870 GCATTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2107 GTATAC GTA^TAC
CelII 1166 GCTCAGC GC^T[ACGT]AGC
EcoNI 1371 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1428 GAATTC G^AATTC
FspI 164 TGCGCA TGC^GCA
HaeII 1401 AGCGCC [GA]GCGC^[CT]
HgaI 401 GCGTC GACGC(5/10)
Hin1I 400 GGCGTC G[GA]^CG[CT]C
KspI 3584 CCGCGG CCGC^GG
MluNI 3447 TGGCCA TGG^CCA
MscI 3447 TGGCCA TGG^CCA
NheI 2940 GCTAGC G^CTAGC
PaeR7I 1330 CTCGAG C^TCGAG
PflFI 3099 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3334 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4291 ATTAAT AT^TAAT
Psp1406I 17 AACGTT AA^CGTT
RcaI 93 TCATGA T^CATGA
SacII 3584 CCGCGG CCGC^GG
SalI 2042 GTCGAC G^TCGAC
Tth111I 3099 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1330 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 737 GGTACC G^GTACC
3480 GGTACC
AccII 324 CGCG CG^CG
3585 CGCG
Asp718I 737 GGTACC G^GTACC
3480 GGTACC
BanII 1164 GGGCTC G[GA]GC[CT]^C
3344 GAGCCC
BglII 428 AGATCT A^GATCT
3878 AGATCT
BssSI 520 CTCGTG CACGAG(-5/-1)
1159 CTCGTG
BstBI 251 TTCGAA TT^CGAA
345 TTCGAA
BstUI 324 CGCG CG^CG
3585 CGCG
EaeI 1060 CGGCCA [CT]^GGCC[GA]
3447 TGGCCA
EcoT22I 628 ATGCAT ATGCA^T
3925 ATGCAT
FauI 396 GCGGG CCCGC(4/6)
1047 GCGGG
KpnI 737 GGTACC GGTAC^C
3480 GGTACC
MslI 262 CATACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4026 CACAGAGATG
MvnI 324 CGCG CG^CG
3585 CGCG
NcoI 84 CCATGG C^CATGG
919 CCATGG
NsiI 628 ATGCAT ATGCA^T
3925 ATGCAT
NspV 251 TTCGAA TT^CGAA
345 TTCGAA
SfuI 251 TTCGAA TT^CGAA
345 TTCGAA
SmaI 1172 CCCGGG CCC^GGG
2680 CCCGGG
SphI 1446 GCATGC GCATG^C
2615 GCATGC
XcmI 2039 CCAGTCGACGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3354 CCAGGCAATCTTTGG
XmaCI 1172 CCCGGG C^CCGGG
2680 CCCGGG
XmaI 1172 CCCGGG C^CCGGG
2680 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanI BbsI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI