##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Aor51HI AscI Asp700I AviII AvrII
BbrPI BcgI BciVI BfrI BlnI
BsaAI BsaBI BsiWI BsmI BspDI
BspHI BsrDI BssSI Bst1107I BstZ17I
ClaI CpoI DrdI Eco47III EcoRI
EcoT22I FseI FspI HpaI KspI
MamI MluI NdeI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SgrAI SnaBI Sse8387I
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2330 GGTACC G^GTACC
AccIII 1402 TCCGGA T^CCGGA
AflIII 390 ACATGT A^C[GA][CT]GT
AhdI 1414 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2889 GTGCAC G^TGCAC
ApaI 232 GGGCCC GGGCC^C
ApaLI 2889 GTGCAC G^TGCAC
Asp718I 2330 GGTACC G^GTACC
AspEI 1414 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2889 GTGCAC G[AT]GC[AT]^C
AspI 2743 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 1043 TGATCA T^GATCA
BglII 3008 AGATCT A^GATCT
BseAI 1402 TCCGGA T^CCGGA
BsiHKAI 2889 GTGCAC G[AT]GC[AT]^C
BspEI 1402 TCCGGA T^CCGGA
BspLU11I 390 ACATGT A^CATGT
BsrBI 222 GAGCGG CCGCTC(-3/-3)
BsrFI 195 GCCGGC [GA]^CCGG[CT]
BssHII 185 GCGCGC G^CGCGC
BstBI 3265 TTCGAA TT^CGAA
BstEII 1556 GGTCACC G^GT[ACGT]ACC
BstPI 1556 GGTCACC G^GT[ACGT]ACC
Cfr10I 195 GCCGGC [GA]^CCGG[CT]
Eam1105I 1414 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2382 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1556 GGTCACC G^GT[ACGT]ACC
EcoRV 1216 GATATC GAT^ATC
FbaI 1043 TGATCA T^GATCA
HgaI 542 GCGTC GACGC(5/10)
HindIII 3996 AAGCTT A^AGCTT
KpnI 2330 GGTACC GGTAC^C
MfeI 696 CAATTG C^AATTG
MroI 1402 TCCGGA T^CCGGA
MunI 696 CAATTG C^AATTG
NaeI 195 GCCGGC GCC^GGC
NgoMIV 195 GCCGGC G^CCGGC
NheI 1431 GCTAGC G^CTAGC
NspV 3265 TTCGAA TT^CGAA
PflFI 2743 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3513 GGGTCCT [GA]G^G[AT]CC[CT]
ScaI 2473 AGTACT AGT^ACT
SfuI 3265 TTCGAA TT^CGAA
SpeI 1077 ACTAGT A^CTAGT
SphI 1408 GCATGC GCATG^C
Tth111I 2743 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccII 186 CGCG CG^CG
3724 CGCG
AcyI 258 GGCGCC G[GA]^CG[CT]C
3072 GGCGCC
AseI 939 ATTAAT AT^TAAT
3924 ATTAAT
AsnI 939 ATTAAT AT^TAAT
3924 ATTAAT
BamHI 3295 GGATCC G^GATCC
3383 GGATCC
BanII 232 GGGCCC G[GA]GC[CT]^C
2378 GAGCCC
BbeI 258 GGCGCC GGCGC^C
3072 GGCGCC
BbsI 762 GAAGAC GAAGAC(2/6)
4093 GTCTTC
BpuAI 762 GAAGAC GAAGAC(2/6)
4093 GTCTTC
BsaHI 258 GGCGCC G[GA]^CG[CT]C
3072 GGCGCC
BsaI 416 GAGACC GGTCTC(1/5)
2620 GAGACC
BsaWI 991 ACCGGA [AT]^CCGG[AT]
1402 TCCGGA
BsgI 2735 CTGCAC GTGCAG(16/14)
3629 GTGCAG
Bsp1407I 2626 TGTACA T^GTACA
3178 TGTACA
BspMI 2357 ACCTGC ACCTGC(4/8)
3821 ACCTGC
BsrGI 2626 TGTACA T^GTACA
3178 TGTACA
BstUI 186 CGCG CG^CG
3724 CGCG
DraI 3899 TTTAAA TTT^AAA
4301 TTTAAA
DraII 2140 AGGGCCT [GA]G^G[ACGT]CC[CT]
3513 GGGTCCT
EcoO109I 2140 AGGGCCT [GA]G^G[ACGT]CC[CT]
3513 GGGTCCT
Hin1I 258 GGCGCC G[GA]^CG[CT]C
3072 GGCGCC
HincII 2876 GTCAAC GT[CT]^[GA]AC
3533 GTTGAC
HindII 2876 GTCAAC GT[CT]^[GA]AC
3533 GTTGAC
KasI 258 GGCGCC G^GCGCC
3072 GGCGCC
MaeII 253 ACGT A^CGT
2027 ACGT
MvnI 186 CGCG CG^CG
3724 CGCG
NarI 258 GGCGCC GG^CGCC
3072 GGCGCC
PflMI 3317 CCAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3662 CCAGCTCCTGG
PshBI 939 ATTAAT AT^TAAT
3924 ATTAAT
PstI 1768 CTGCAG CTGCA^G
2447 CTGCAG
SexAI 1552 ACCAGGT A^CC[AT]GGT
2498 ACCAGGT
SfiI 198 GGCCGTCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3783 GGCCAGGCTGGCC
SfoI 258 GGCGCC GGC^GCC
3072 GGCGCC
SmaI 146 CCCGGG CCC^GGG
235 CCCGGG
SspBI 2626 TGTACA T^GTACA
3178 TGTACA
StuI 2186 AGGCCT AGG^CCT
2312 AGGCCT
TaqI 1991 TCGA T^CGA
3266 TCGA
TthHB8I 1991 TCGA T^CGA
3266 TCGA
Van91I 3317 CCAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3662 CCAGCTCCTGG
XmaCI 146 CCCGGG C^CCGGG
235 CCCGGG
XmaI 146 CCCGGG C^CCGGG
235 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AvaI AvaII
BalI BanI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsiEI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraIII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII