##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AgeI AhdI AscI Asp700I Asp718I
AspEI AspI AvrII BbrPI BbvCI
BclI BfrI BglII BlnI BseAI
BsiEI BspDI BspEI BspHI BssHII
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MluI
MroI NdeI NotI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RcaI RsrII
SalI SfuI SgrAI SpeI SspI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AclI 1539 AACGTT AA^CGTT
Alw44I 1986 GTGCAC G^TGCAC
AocI 4024 CCTGAGG CC^T[ACGT]AGG
ApaLI 1986 GTGCAC G^TGCAC
AseI 2083 ATTAAT AT^TAAT
AsnI 2083 ATTAAT AT^TAAT
AviII 671 TGCGCA TGC^GCA
BamHI 3783 GGATCC G^GATCC
BbeI 1630 GGCGCC GGCGC^C
BsiWI 1388 CGTACG C^GTACG
BspLU11I 1796 ACATGT A^CATGT
BsrDI 3446 CATTGC GCAATG(2/0)
Bsu36I 4024 CCTGAGG CC^T[ACGT]AGG
DraIII 1900 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 4024 CCTGAGG CC^T[ACGT]AGG
EcoNI 1974 CCTTGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 671 TGCGCA TGC^GCA
KasI 1630 GGCGCC G^GCGCC
MfeI 624 CAATTG C^AATTG
MunI 624 CAATTG C^AATTG
NarI 1630 GGCGCC GG^CGCC
NcoI 1190 CCATGG C^CATGG
NheI 3135 GCTAGC G^CTAGC
NruI 4153 TCGCGA TCG^CGA
PshBI 2083 ATTAAT AT^TAAT
Psp1406I 1539 AACGTT AA^CGTT
SapI 2178 GAAGAGC GCTCTTC(1/4)
SbfI 609 CCTGCAGG CCTGCA^GG
ScaI 1841 AGTACT AGT^ACT
SexAI 2381 ACCTGGT A^CC[AT]GGT
SfiI 2547 GGCCTTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1630 GGCGCC GGC^GCC
SnaBI 2161 TACGTA TAC^GTA
SphI 2591 GCATGC GCATG^C
Sse8387I 609 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 3596 AAATTT [GA]^AATT[CT]
3995 AAATTC
ApoI 3596 AAATTT [GA]^AATT[CT]
3995 AAATTC
BcgI 1326 CGAGGGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2507 GCAGGAGGATCG
BsaBI 2514 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2784 GATTGCCATC
BsaI 2740 GAGACC GGTCTC(1/5)
717 GGTCTC
BsmI 77 GAATGC GAATGC(1/-1)
1877 GCATTC
Bsp1407I 1799 TGTACA T^GTACA
2420 TGTACA
BsrBI 1105 GAGCGG CCGCTC(-3/-3)
1810 GAGCGG
BsrGI 1799 TGTACA T^GTACA
2420 TGTACA
BstXI 244 CCAAACCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1526 CCACCACAGTGG
DrdI 2110 GACCAGTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2380 GACCTGGTGGTC
EcoT14I 1190 CCATGG C^C[AT][AT]GG
1979 CCAAGG
HincII 62 GTCAAC GT[CT]^[GA]AC
790 GTCAAC
HindII 62 GTCAAC GT[CT]^[GA]AC
790 GTCAAC
KspI 1211 CCGCGG CCGC^GG
2907 CCGCGG
MamI 2514 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2784 GATTGCCATC
NaeI 1617 GCCGGC GCC^GGC
4118 GCCGGC
NgoMIV 1617 GCCGGC G^CCGGC
4118 GCCGGC
NspI 1796 ACATGT [GA]CATG^[CT]
2591 GCATGC
SacI 868 GAGCTC GAGCT^C
3325 GAGCTC
SacII 1211 CCGCGG CCGC^GG
2907 CCGCGG
SmaI 2647 CCCGGG CCC^GGG
3399 CCCGGG
SspBI 1799 TGTACA T^GTACA
2420 TGTACA
StyI 1190 CCATGG C^C[AT][AT]GG
1979 CCAAGG
XmaCI 2647 CCCGGG C^CCGGG
3399 CCCGGG
XmaI 2647 CCCGGG C^CCGGG
3399 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III EcoO109I
EcoO65I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PaeR7I PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoI XhoII