##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AscI AseI AsnI AspEI AvrII
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsiEI BsiWI Bsp1407I
BspDI BspHI BsrGI BssHII BstBI
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoRV EcoT22I FbaI FseI KspI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 3294 TCCGGA T^CCGGA
AcyI 3925 GGCGCC G[GA]^CG[CT]C
Alw44I 4777 GTGCAC G^TGCAC
ApaI 4001 GGGCCC GGGCC^C
ApaLI 4777 GTGCAC G^TGCAC
AspI 2389 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4146 TGCGCA TGC^GCA
BbeI 3925 GGCGCC GGCGC^C
BbrPI 4780 CACGTG CAC^GTG
BglII 4209 AGATCT A^GATCT
BsaAI 4780 CACGTG [CT]AC^GT[GA]
BsaHI 3925 GGCGCC G[GA]^CG[CT]C
BsaI 572 GAGACC GGTCTC(1/5)
BsaWI 3294 TCCGGA [AT]^CCGG[AT]
BseAI 3294 TCCGGA T^CCGGA
BsmI 2102 GAATGC GAATGC(1/-1)
BspEI 3294 TCCGGA T^CCGGA
BsrBI 4858 CCGCTC CCGCTC(-3/-3)
BsrFI 3256 ACCGGC [GA]^CCGG[CT]
BssSI 4283 CTCGTG CACGAG(-5/-1)
Bst1107I 1039 GTATAC GTA^TAC
BstEII 956 GGTGACC G^GT[ACGT]ACC
BstPI 956 GGTGACC G^GT[ACGT]ACC
BstZ17I 1039 GTATAC GTA^TAC
Cfr10I 3256 ACCGGC [GA]^CCGG[CT]
EcoO65I 956 GGTGACC G^GT[ACGT]ACC
EcoRI 418 GAATTC G^AATTC
FspI 4146 TGCGCA TGC^GCA
Hin1I 3925 GGCGCC G[GA]^CG[CT]C
HindIII 1742 AAGCTT A^AGCTT
HpaI 1825 GTTAAC GTT^AAC
KasI 3925 GGCGCC G^GCGCC
MroI 3294 TCCGGA T^CCGGA
NarI 3925 GGCGCC GG^CGCC
PaeR7I 617 CTCGAG C^TCGAG
PflFI 2389 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 895 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4780 CACGTG CAC^GTG
PmlI 4780 CACGTG CAC^GTG
PshAI 3389 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 4378 GCTCTTC GCTCTTC(1/4)
ScaI 774 AGTACT AGT^ACT
SexAI 3268 ACCAGGT A^CC[AT]GGT
SfiI 2641 GGCCATCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3925 GGCGCC GGC^GCC
SmaI 2376 CCCGGG CCC^GGG
Tth111I 2389 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 895 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 617 CTCGAG C^TCGAG
XmaCI 2376 CCCGGG C^CCGGG
XmaI 2376 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Acc65I 227 GGTACC G^GTACC
830 GGTACC
AflIII 693 ACATGT A^C[GA][CT]GT
2036 ACATGT
Aor51HI 1194 AGCGCT AGC^GCT
2445 AGCGCT
Asp718I 227 GGTACC G^GTACC
830 GGTACC
AvaI 617 CTCGAG C^[CT]CG[GA]G
2376 CCCGGG
BamHI 448 GGATCC G^GATCC
1498 GGATCC
BglI 2642 GCCATCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4686 GCCCCAGTGGC
BsoBI 617 CTCGAG C^[CT]CG[GA]G
2376 CCCGGG
BspLU11I 693 ACATGT A^CATGT
2036 ACATGT
BstXI 2180 CCAAATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4639 CCAGGCCTCTGG
Eco47III 1194 AGCGCT AGC^GCT
2445 AGCGCT
EcoNI 1228 CCTAACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4525 CCTGAAGAAGG
HincII 1379 GTCAAC GT[CT]^[GA]AC
1825 GTTAAC
HindII 1379 GTCAAC GT[CT]^[GA]AC
1825 GTTAAC
KpnI 227 GGTACC GGTAC^C
830 GGTACC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI