##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BcnI
BglI BlnI BsaHI BsaWI BseAI
BsiWI BsmBI BspEI BspHI BsrFI
BssHII BstBI BstUI BstXI CfoI
Cfr10I CpoI Eam1105I Eco47III EcoNI
EcoRI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaII
KasI KpnI KspI MluI MluNI
MroI MscI MspI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
RcaI RsrII SacI SacII SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AocI 3375 CCTCAGG CC^T[ACGT]AGG
AspHI 137 GAGCAC G[AT]GC[AT]^C
BciVI 1143 GTATCC GTATCC(6/5)
BglII 928 AGATCT A^GATCT
BlpI 629 GCTCAGC GC^T[ACGT]AGC
BmyI 137 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 629 GCTCAGC GC^T[ACGT]AGC
BsaBI 2671 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1582 CGGCCG CG[GA][CT]^CG
BsiHKAI 137 GAGCAC G[AT]GC[AT]^C
Bsp1286I 137 GAGCAC G[AGT]GC[ACT]^C
BsrBI 3285 GAGCGG CCGCTC(-3/-3)
BssSI 3066 CACGAG CACGAG(-5/-1)
Bst1107I 3321 GTATAC GTA^TAC
BstAPI 959 GCAACTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3321 GTATAC GTA^TAC
Bsu36I 3375 CCTCAGG CC^T[ACGT]AGG
CelII 629 GCTCAGC GC^T[ACGT]AGC
DraIII 2226 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2776 GACACCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1582 CGGCCG [CT]^GGCC[GA]
EagI 1582 CGGCCG C^GGCCG
EclXI 1582 CGGCCG C^GGCCG
Eco52I 1582 CGGCCG C^GGCCG
Eco81I 3375 CCTCAGG CC^T[ACGT]AGG
FauI 3287 GCGGG CCCGC(4/6)
HpaI 2155 GTTAAC GTT^AAC
MamI 2671 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1536 CAATTG C^AATTG
MspA1I 611 CAGCTG C[AC]G^C[GT]G
MunI 1536 CAATTG C^AATTG
PaeR7I 635 CTCGAG C^TCGAG
PvuII 611 CAGCTG CAG^CTG
SalI 2999 GTCGAC G^TCGAC
SpeI 2326 ACTAGT A^CTAGT
SphI 3744 GCATGC GCATG^C
StuI 1408 AGGCCT AGG^CCT
XcmI 4061 CCAGGGTGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 635 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AciI 1581 GCGG CCGC(-3/-1)
3287 GCGG
AclI 98 AACGTT AA^CGTT
2358 AACGTT
Asp700I 2285 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2660 GAAAAAATTC
AvaI 635 CTCGAG C^[CT]CG[GA]G
1872 CCCGAG
BanI 1184 GGCACC G^G[CT][GA]CC
2183 GGCACC
BbsI 2249 GAAGAC GAAGAC(2/6)
2760 GTCTTC
BclI 1978 TGATCA T^GATCA
4401 TGATCA
BpuAI 2249 GAAGAC GAAGAC(2/6)
2760 GTCTTC
BsaI 885 GAGACC GGTCTC(1/5)
3292 GAGACC
BsgI 1137 CTGCAC GTGCAG(16/14)
620 GTGCAG
BsmI 3588 GAATGC GAATGC(1/-1)
4432 GCATTC
BsoBI 635 CTCGAG C^[CT]CG[GA]G
1872 CCCGAG
BspDI 2019 ATCGAT AT^CGAT
3050 ATCGAT
BspLU11I 2589 ACATGT A^CATGT
3012 ACATGT
BspMI 1863 ACCTGC ACCTGC(4/8)
1884 ACCTGC
BstEII 3871 GGTGACC G^GT[ACGT]ACC
4075 GGTAACC
BstPI 3871 GGTGACC G^GT[ACGT]ACC
4075 GGTAACC
ClaI 2019 ATCGAT AT^CGAT
3050 ATCGAT
EcoO65I 3871 GGTGACC G^GT[ACGT]ACC
4075 GGTAACC
EcoRV 1127 GATATC GAT^ATC
2396 GATATC
EcoT14I 1674 CCATGG C^C[AT][AT]GG
2712 CCATGG
FbaI 1978 TGATCA T^GATCA
4401 TGATCA
HincII 2155 GTTAAC GT[CT]^[GA]AC
2999 GTCGAC
HindII 2155 GTTAAC GT[CT]^[GA]AC
2999 GTCGAC
HindIII 269 AAGCTT A^AGCTT
2524 AAGCTT
NcoI 1674 CCATGG C^CATGG
2712 CCATGG
PleI 1226 GACTC GAGTC(4/5)
2036 GAGTC
Psp1406I 98 AACGTT AA^CGTT
2358 AACGTT
StyI 1674 CCATGG C^C[AT][AT]GG
2712 CCATGG
XbaI 931 TCTAGA T^CTAGA
4196 TCTAGA
XmnI 2285 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2660 GAAAAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI AseI
AsnI AvaII BbvI BfaI BfrI
BpmI BsaAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT22I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeI NdeII NlaIII NlaIV NsiI
NspI PflMI PpuMI PshBI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII