##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccI AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AvrII BbeI BbrPI BlnI
BseAI BsiWI BspEI BspMI BsrBI
BsrFI BssHII Bst1107I BstZ17I Cfr10I
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FseI HpaI KasI
KspI MluI MroI NaeI NarI
NgoMIV NotI NruI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 477 GACGTC GACGT^C
AccII 3986 CGCG CG^CG
AcyI 477 GACGTC G[GA]^CG[CT]C
AflII 3139 CTTAAG C^TTAAG
AocI 3919 CCTGAGG CC^T[ACGT]AGG
Asp700I 5580 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 258 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3333 CTCGGG C^[CT]CG[GA]G
BamHI 1217 GGATCC G^GATCC
BcgI 4163 CGAGCCAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3139 CTTAAG C^TTAAG
BglI 6329 GCCATACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3724 AGATCT A^GATCT
BsaAI 5530 TACGTG [CT]AC^GT[GA]
BsaHI 477 GACGTC G[GA]^CG[CT]C
BsaWI 5199 TCCGGT [AT]^CCGG[AT]
BseRI 1467 CTCCTC GAGGAG(10/8)
BsiEI 5442 CGGTCG CG[GA][CT]^CG
BsmBI 256 GAGACG CGTCTC(1/5)
BsoBI 3333 CTCGGG C^[CT]CG[GA]G
BspDI 2607 ATCGAT AT^CGAT
BspLU11I 4836 ACATGT A^CATGT
BssSI 4214 CTCGTG CACGAG(-5/-1)
BstBI 3294 TTCGAA TT^CGAA
BstUI 3986 CGCG CG^CG
Bsu36I 3919 CCTGAGG CC^T[ACGT]AGG
ClaI 2607 ATCGAT AT^CGAT
Eco81I 3919 CCTGAGG CC^T[ACGT]AGG
EcoRV 5123 GATATC GAT^ATC
FauI 2480 GCGGG CCCGC(4/6)
HaeII 4603 AGCGCC [GA]GCGC^[CT]
Hin1I 477 GACGTC G[GA]^CG[CT]C
HincII 1293 GTCAAC GT[CT]^[GA]AC
HindII 1293 GTCAAC GT[CT]^[GA]AC
MvnI 3986 CGCG CG^CG
NdeI 3477 CATATG CA^TATG
NheI 491 GCTAGC G^CTAGC
NspV 3294 TTCGAA TT^CGAA
PacI 6289 TTAATTAA TTAAT^TAA
PflFI 258 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfuI 3294 TTCGAA TT^CGAA
SphI 10 GCATGC GCATG^C
Tth111I 258 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 5580 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 5202 GGTACC G^GTACC
5831 GGTACC
AflIII 4836 ACATGT A^C[GA][CT]GT
5531 ACGTGT
Asp718I 5202 GGTACC G^GTACC
5831 GGTACC
AviII 4965 TGCGCA TGC^GCA
5059 TGCGCA
BanII 1646 GAGCCC G[GA]GC[CT]^C
6044 GGGCTC
BbvCI 2799 CCTCAGC CCTCAGC(-5/-2)
6147 CCTCAGC
BclI 49 TGATCA T^GATCA
1980 TGATCA
BcnI 3472 CCGGG CC^[GC]GG
4863 CCCGG
BsaBI 2291 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4919 GATTCCTATC
BstAPI 2548 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5850 GCAGCTGATGC
BstEII 1495 GGTAACC G^GT[ACGT]ACC
1537 GGTCACC
BstPI 1495 GGTAACC G^GT[ACGT]ACC
1537 GGTCACC
DraIII 162 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
3508 CACAAGGTG
DrdI 856 GACGGACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2459 GACATCAATGTC
EcoNI 2281 CCTCCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4648 CCTCTACAAGG
EcoO65I 1495 GGTAACC G^GT[ACGT]ACC
1537 GGTCACC
FbaI 49 TGATCA T^GATCA
1980 TGATCA
FspI 4965 TGCGCA TGC^GCA
5059 TGCGCA
HgaI 2988 GACGC GACGC(5/10)
2557 GCGTC
KpnI 5202 GGTACC GGTAC^C
5831 GGTACC
MamI 2291 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4919 GATTCCTATC
NciI 3472 CCGGG CC^[GC]GG
4863 CCCGG
NcoI 408 CCATGG C^CATGG
3488 CCATGG
PpuMI 4272 AGGTCCT [GA]G^G[AT]CC[CT]
5972 GGGACCT
PshAI 1362 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3137 GACTTAAGTC
SapI 1724 GAAGAGC GCTCTTC(1/4)
3290 GCTCTTC
SspI 4596 AATATT AAT^ATT
5938 AATATT
XbaI 1370 TCTAGA T^CTAGA
5540 TCTAGA
XcmI 2138 CCACCAAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6315 CCACTGAATGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaII BalI BanI BbsI BbvI
BciVI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspHI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PshBI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII