##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbvCI BcgI BciVI BfrI
BlnI BlpI Bpu1102I BsaBI BsiWI
BsmBI BspDI BspLU11I Bst1107I BstAPI
BstEII BstPI BstZ17I CelII ClaI
CpoI Eam1105I Eco47III EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HindIII
HpaI KpnI KspI MamI MluI
NaeI NgoMIV NheI NotI NruI
NsiI PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SgrAI SnaBI Sse8387I
SspI StuI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 1987 GTCTAC GT^[AC][GT]AC
AccIII 1903 TCCGGA T^CCGGA
Alw44I 1488 GTGCAC G^TGCAC
AocI 603 CCTGAGG CC^T[ACGT]AGG
ApaI 184 GGGCCC GGGCC^C
ApaLI 1488 GTGCAC G^TGCAC
BanI 315 GGCGCC G^G[CT][GA]CC
BanII 184 GGGCCC G[GA]GC[CT]^C
BbeI 315 GGCGCC GGCGC^C
BclI 3509 TGATCA T^GATCA
BglI 2943 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 890 AGATCT A^GATCT
BseAI 1903 TCCGGA T^CCGGA
Bsp1407I 2925 TGTACA T^GTACA
BspEI 1903 TCCGGA T^CCGGA
BspHI 3775 TCATGA T^CATGA
BspMI 364 ACCTGC ACCTGC(4/8)
BsrGI 2925 TGTACA T^GTACA
BstBI 2227 TTCGAA TT^CGAA
BstXI 2953 CCATTCGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 603 CCTGAGG CC^T[ACGT]AGG
DraIII 2644 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2730 GACAAGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 20 CGGCCG C^GGCCG
EclXI 20 CGGCCG C^GGCCG
Eco52I 20 CGGCCG C^GGCCG
Eco81I 603 CCTGAGG CC^T[ACGT]AGG
FbaI 3509 TGATCA T^GATCA
HgaI 203 GACGC GACGC(5/10)
KasI 315 GGCGCC G^GCGCC
MfeI 2816 CAATTG C^AATTG
MroI 1903 TCCGGA T^CCGGA
MunI 2816 CAATTG C^AATTG
NarI 315 GGCGCC GG^CGCC
NcoI 1108 CCATGG C^CATGG
NdeI 2758 CATATG CA^TATG
NspV 2227 TTCGAA TT^CGAA
PaeR7I 2500 CTCGAG C^TCGAG
RcaI 3775 TCATGA T^CATGA
SapI 1461 GAAGAGC GCTCTTC(1/4)
SfoI 315 GGCGCC GGC^GCC
SfuI 2227 TTCGAA TT^CGAA
SpeI 884 ACTAGT A^CTAGT
SspBI 2925 TGTACA T^GTACA
XbaI 3376 TCTAGA T^CTAGA
XhoI 2500 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 203 GACGCC G[GA]^CG[CT]C
315 GGCGCC
AflIII 2922 ACGTGT A^C[GA][CT]GT
2928 ACACGT
AlwNI 624 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
2173 CAGAGGCTG
BalI 2113 TGGCCA TGG^CCA
2950 TGGCCA
BsaHI 203 GACGCC G[GA]^CG[CT]C
315 GGCGCC
BsaI 1387 GAGACC GGTCTC(1/5)
1413 GGTCTC
BsiEI 20 CGGCCG CG[GA][CT]^CG
2061 CGGTCG
BsmI 2366 GAATGC GAATGC(1/-1)
3879 GCATTC
BsrBI 2291 CCGCTC CCGCTC(-3/-3)
163 GAGCGG
BsrDI 1545 GCAATG GCAATG(2/0)
3503 GCAATG
BsrFI 312 ACCGGC [GA]^CCGG[CT]
3253 GCCGGT
BssHII 389 GCGCGC G^CGCGC
391 GCGCGC
BssSI 933 CTCGTG CACGAG(-5/-1)
1289 CTCGTG
BstYI 890 AGATCT [GA]^GATC[CT]
1586 AGATCC
Cfr10I 312 ACCGGC [GA]^CCGG[CT]
3253 GCCGGT
DraI 1628 TTTAAA TTT^AAA
3522 TTTAAA
EcoRI 2721 GAATTC G^AATTC
2795 GAATTC
Hin1I 203 GACGCC G[GA]^CG[CT]C
315 GGCGCC
HincII 2043 GTCAAC GT[CT]^[GA]AC
2238 GTTGAC
HindII 2043 GTCAAC GT[CT]^[GA]AC
2238 GTTGAC
MflI 890 AGATCT [GA]^GATC[CT]
1586 AGATCC
MluNI 2113 TGGCCA TGG^CCA
2950 TGGCCA
MscI 2113 TGGCCA TGG^CCA
2950 TGGCCA
PflMI 1103 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1130 CCAGCACGTGG
PpuMI 2299 GGGACCT [GA]G^G[AT]CC[CT]
2435 GGGTCCT
SmaI 187 CCCGGG CCC^GGG
471 CCCGGG
SphI 1068 GCATGC GCATG^C
2629 GCATGC
Van91I 1103 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1130 CCAGCACGTGG
XcmI 2101 CCAAACTTAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3553 CCAGAATGTCCCTGG
XhoII 890 AGATCT [GA]^GATC[CT]
1586 AGATCC
XmaCI 187 CCCGGG C^CCGGG
471 CCCGGG
XmaI 187 CCCGGG C^CCGGG
471 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BbrPI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PmaCI PmlI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI