##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccII AccIII AhdI AscI
AspEI AviII AvrII BalI BbrPI
BglI BlnI BsaAI BseAI BsiEI
BsiWI BsmBI BspEI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CpoI DrdI EaeI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
EcoT22I FseI FspI KspI MluI
MluNI MroI MscI MvnI NaeI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PvuI RsrII SacII SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI Sse8387I StuI SwaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AflII 6093 CTTAAG C^TTAAG
AspI 2025 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1848 CTCGGG C^[CT]CG[GA]G
BbeI 1935 GGCGCC GGCGC^C
BclI 4500 TGATCA T^GATCA
BfrI 6093 CTTAAG C^TTAAG
BsoBI 1848 CTCGGG C^[CT]CG[GA]G
Bsp1407I 306 TGTACA T^GTACA
BspDI 3263 ATCGAT AT^CGAT
BspMI 1182 ACCTGC ACCTGC(4/8)
BsrGI 306 TGTACA T^GTACA
ClaI 3263 ATCGAT AT^CGAT
FbaI 4500 TGATCA T^GATCA
HindIII 6048 AAGCTT A^AGCTT
HpaI 3804 GTTAAC GTT^AAC
KasI 1935 GGCGCC G^GCGCC
MfeI 1022 CAATTG C^AATTG
MunI 1022 CAATTG C^AATTG
NarI 1935 GGCGCC GG^CGCC
PflFI 2025 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4516 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 5834 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 5780 GAGCTC GAGCT^C
SalI 5831 GTCGAC G^TCGAC
SapI 3478 GAAGAGC GCTCTTC(1/4)
ScaI 225 AGTACT AGT^ACT
SfoI 1935 GGCGCC GGC^GCC
SphI 4526 GCATGC GCATG^C
SspBI 306 TGTACA T^GTACA
Tth111I 2025 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4516 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 1428 GGTACC G^GTACC
2689 GGTACC
AclI 3041 AACGTT AA^CGTT
7246 AACGTT
AcyI 19 GACGCC G[GA]^CG[CT]C
1935 GGCGCC
AgeI 3290 ACCGGT A^CCGGT
3323 ACCGGT
Alw44I 4746 GTGCAC G^TGCAC
7350 GTGCAC
ApaLI 4746 GTGCAC G^TGCAC
7350 GTGCAC
AseI 4483 ATTAAT AT^TAAT
5206 ATTAAT
AsnI 4483 ATTAAT AT^TAAT
5206 ATTAAT
Asp700I 3844 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6604 GAATGGGTTC
Asp718I 1428 GGTACC G^GTACC
2689 GGTACC
BbvCI 1157 CCTCAGC CCTCAGC(-5/-2)
4567 GCTGAGG
BciVI 4758 GGATAC GTATCC(6/5)
5055 GGATAC
BsaHI 19 GACGCC G[GA]^CG[CT]C
1935 GGCGCC
BsaWI 3290 ACCGGT [AT]^CCGG[AT]
3323 ACCGGT
BsmI 4049 GCATTC GAATGC(1/-1)
4545 GCATTC
BspHI 450 TCATGA T^CATGA
2838 TCATGA
BspLU11I 3981 ACATGT A^CATGT
4467 ACATGT
BsrFI 3290 ACCGGT [GA]^CCGG[CT]
3323 ACCGGT
BstAPI 1098 GCAGGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1374 GCAGGAATTGC
Cfr10I 3290 ACCGGT [GA]^CCGG[CT]
3323 ACCGGT
DraIII 6764 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
6782 CACAAAGTG
EcoNI 176 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5173 CCTGATTCAGG
EcoRI 6544 GAATTC G^AATTC
7239 GAATTC
Hin1I 19 GACGCC G[GA]^CG[CT]C
1935 GGCGCC
KpnI 1428 GGTACC GGTAC^C
2689 GGTACC
NheI 2229 GCTAGC G^CTAGC
2259 GCTAGC
PinAI 3290 ACCGGT A^CCGGT
3323 ACCGGT
PpuMI 2947 AGGTCCT [GA]G^G[AT]CC[CT]
5283 AGGACCT
PshBI 4483 ATTAAT AT^TAAT
5206 ATTAAT
Psp1406I 3041 AACGTT AA^CGTT
7246 AACGTT
RcaI 450 TCATGA T^CATGA
2838 TCATGA
SpeI 4687 ACTAGT A^CTAGT
4846 ACTAGT
SspI 5194 AATATT AAT^ATT
6490 AATATT
XmnI 3844 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6604 GAATGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI Aor51HI
ApaI ApoI AspHI AvaII BamHI
BanI BanII BbsI BbvI BcgI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI Eco47III Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII