##################################
# NO CUT ENZYMES ( 37 enzymes) #
##################################
Acc65I AflII AgeI AhdI AscI
Asp718I AspEI BfrI BsiWI BsrBI
BsrDI Bst1107I BstBI BstZ17I CpoI
DrdI Eam1105I FseI KpnI NheI
NotI NruI NspV PacI PinAI
PmeI PshAI PvuI RsrII SalI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 596 AACGTT AA^CGTT
Aor51HI 4722 AGCGCT AGC^GCT
AseI 2332 ATTAAT AT^TAAT
AsnI 2332 ATTAAT AT^TAAT
AvrII 3542 CCTAGG C^CTAGG
BamHI 4433 GGATCC G^GATCC
BbeI 2302 GGCGCC GGCGC^C
BbvCI 7252 GCTGAGG CCTCAGC(-5/-2)
BciVI 4019 GGATAC GTATCC(6/5)
BclI 816 TGATCA T^GATCA
BglII 1858 AGATCT A^GATCT
BlnI 3542 CCTAGG C^CTAGG
BsaBI 4226 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 778 ATCGAT AT^CGAT
BspHI 6702 TCATGA T^CATGA
BssHII 3766 GCGCGC G^CGCGC
BstEII 3151 GGTGACC G^GT[ACGT]ACC
BstPI 3151 GGTGACC G^GT[ACGT]ACC
ClaI 778 ATCGAT AT^CGAT
DraIII 4950 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4722 AGCGCT AGC^GCT
EcoO65I 3151 GGTGACC G^GT[ACGT]ACC
EcoRI 3356 GAATTC G^AATTC
EcoRV 521 GATATC GAT^ATC
FbaI 816 TGATCA T^GATCA
HpaI 5406 GTTAAC GTT^AAC
KasI 2302 GGCGCC G^GCGCC
KspI 1986 CCGCGG CCGC^GG
MamI 4226 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2370 CAATTG C^AATTG
MunI 2370 CAATTG C^AATTG
NarI 2302 GGCGCC GG^CGCC
PflMI 4949 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2332 ATTAAT AT^TAAT
Psp1406I 596 AACGTT AA^CGTT
RcaI 6702 TCATGA T^CATGA
SacII 1986 CCGCGG CCGC^GG
SbfI 7154 CCTGCAGG CCTGCA^GG
ScaI 2556 AGTACT AGT^ACT
SfoI 2302 GGCGCC GGC^GCC
SmaI 7379 CCCGGG CCC^GGG
Sse8387I 7154 CCTGCAGG CCTGCA^GG
Van91I 4949 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4803 TCTAGA T^CTAGA
XmaCI 7379 CCCGGG C^CCGGG
XmaI 7379 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1208 GTCTAC GT^[AC][GT]AC
1370 GTAGAC
AccIII 2144 TCCGGA T^CCGGA
3988 TCCGGA
Asp700I 784 GAATGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
922 GAATCTGTTC
AviII 3329 TGCGCA TGC^GCA
7144 TGCGCA
BcgI 2185 CGACGGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1637 GCAGATTCTTCG
BfaI 3543 CTAG C^TAG
4804 CTAG
BlpI 2092 GCTGAGC GC^T[ACGT]AGC
7498 GCTCAGC
BpmI 2036 CTGGAG CTGGAG(16/14)
4914 CTGGAG
Bpu1102I 2092 GCTGAGC GC^T[ACGT]AGC
7498 GCTCAGC
BsaWI 2144 TCCGGA [AT]^CCGG[AT]
3988 TCCGGA
BseAI 2144 TCCGGA T^CCGGA
3988 TCCGGA
BspEI 2144 TCCGGA T^CCGGA
3988 TCCGGA
CelII 2092 GCTGAGC GC^T[ACGT]AGC
7498 GCTCAGC
EcoT22I 6537 ATGCAT ATGCA^T
6746 ATGCAT
FspI 3329 TGCGCA TGC^GCA
7144 TGCGCA
HindIII 2960 AAGCTT A^AGCTT
5539 AAGCTT
MaeI 3543 CTAG C^TAG
4804 CTAG
MluI 6664 ACGCGT A^CGCGT
7562 ACGCGT
MroI 2144 TCCGGA T^CCGGA
3988 TCCGGA
NcoI 3866 CCATGG C^CATGG
3952 CCATGG
NdeI 6493 CATATG CA^TATG
6690 CATATG
NsiI 6537 ATGCAT ATGCA^T
6746 ATGCAT
PaeR7I 2217 CTCGAG C^TCGAG
3655 CTCGAG
SspI 4812 AATATT AAT^ATT
5069 AATATT
XhoI 2217 CTCGAG C^TCGAG
3655 CTCGAG
XmnI 784 GAATGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
922 GAATCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcnI BglI BmyI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspLU11I BspMI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII