##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AseI AsnI AviII BbrPI
BcgI BclI BfrI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI EagI EclXI Eco52I EcoRV
FbaI FseI FspI HpaI MluI
MroI NdeI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI Sse8387I SspBI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 3072 ACGTGT A^C[GA][CT]GT
AhdI 1538 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4531 GTGCAC G^TGCAC
Aor51HI 2697 AGCGCT AGC^GCT
ApaLI 4531 GTGCAC G^TGCAC
Asp700I 307 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1538 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1465 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3737 CCTAGG C^CTAGG
BglII 4839 AGATCT A^GATCT
BlnI 3737 CCTAGG C^CTAGG
BsaAI 4389 TACGTG [CT]AC^GT[GA]
BsaBI 779 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2813 CGGTCG CG[GA][CT]^CG
BsrBI 3052 CCGCTC CCGCTC(-3/-3)
BstEII 1660 GGTGACC G^GT[ACGT]ACC
BstPI 1660 GGTGACC G^GT[ACGT]ACC
DrdI 473 GACAGAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1538 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2697 AGCGCT AGC^GCT
EcoO65I 1660 GGTGACC G^GT[ACGT]ACC
EcoRI 844 GAATTC G^AATTC
MamI 779 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 69 CAATTG C^AATTG
MunI 69 CAATTG C^AATTG
NheI 518 GCTAGC G^CTAGC
PflFI 1465 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4464 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 2286 ACCTGGT A^CC[AT]GGT
SpeI 3601 ACTAGT A^CTAGT
SphI 2898 GCATGC GCATG^C
SspI 4711 AATATT AAT^ATT
Tth111I 1465 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4698 TCTAGA T^CTAGA
XmnI 307 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 565 GGTACC G^GTACC
1840 GGTACC
AccI 1157 GTCTAC GT^[AC][GT]AC
4500 GTAGAC
Asp718I 565 GGTACC G^GTACC
1840 GGTACC
BamHI 1227 GGATCC G^GATCC
5129 GGATCC
BbsI 1546 GTCTTC GAAGAC(2/6)
1987 GTCTTC
BciVI 4776 GGATAC GTATCC(6/5)
1525 GTATCC
BlpI 406 GCTCAGC GC^T[ACGT]AGC
1937 GCTGAGC
Bpu1102I 406 GCTCAGC GC^T[ACGT]AGC
1937 GCTGAGC
BpuAI 1546 GTCTTC GAAGAC(2/6)
1987 GTCTTC
BsmBI 1279 GAGACG CGTCTC(1/5)
1420 GAGACG
BssSI 1803 CTCGTG CACGAG(-5/-1)
2686 CTCGTG
CelII 406 GCTCAGC GC^T[ACGT]AGC
1937 GCTGAGC
DraIII 901 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1800 CACCTCGTG
EcoT22I 4234 ATGCAT ATGCA^T
4305 ATGCAT
HincII 701 GTCAAC GT[CT]^[GA]AC
1202 GTCAAC
HindII 701 GTCAAC GT[CT]^[GA]AC
1202 GTCAAC
HindIII 136 AAGCTT A^AGCTT
3359 AAGCTT
KpnI 565 GGTACC GGTAC^C
1840 GGTACC
NaeI 114 GCCGGC GCC^GGC
294 GCCGGC
NcoI 1374 CCATGG C^CATGG
2131 CCATGG
NgoMIV 114 GCCGGC G^CCGGC
294 GCCGGC
NsiI 4234 ATGCAT ATGCA^T
4305 ATGCAT
NspI 2898 GCATGC [GA]CATG^[CT]
3751 GCATGT
SacI 1940 GAGCTC GAGCT^C
3327 GAGCTC
SapI 280 GCTCTTC GCTCTTC(1/4)
4288 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI