##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AseI AsnI
Asp700I AspEI AspI AviII BbeI
BfrI BsaBI BseAI BsiWI BspDI
BspEI BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI Eam1105I EcoRV
FspI HpaI KasI MamI MfeI
MluI MroI MseI MunI NarI
NheI NotI NruI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI ScaI
SfoI SfuI SnaBI SpeI SspI
StuI SwaI Tru9I Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1145 GGTACC G^GTACC
AccI 1992 GTCTAC GT^[AC][GT]AC
AocI 4925 CCTGAGG CC^T[ACGT]AGG
Aor51HI 206 AGCGCT AGC^GCT
AscI 1796 GGCGCGCC GG^CGCGCC
Asp718I 1145 GGTACC G^GTACC
AvrII 4048 CCTAGG C^CTAGG
BamHI 2433 GGATCC G^GATCC
BciVI 2757 GTATCC GTATCC(6/5)
BclI 320 TGATCA T^GATCA
BlnI 4048 CCTAGG C^CTAGG
BsaWI 2190 ACCGGA [AT]^CCGG[AT]
Bsp1407I 698 TGTACA T^GTACA
BspHI 5021 TCATGA T^CATGA
BspLU11I 893 ACATGT A^CATGT
BsrGI 698 TGTACA T^GTACA
BssHII 1797 GCGCGC G^CGCGC
BstEII 84 GGTCACC G^GT[ACGT]ACC
BstPI 84 GGTCACC G^GT[ACGT]ACC
Bsu36I 4925 CCTGAGG CC^T[ACGT]AGG
Eco47III 206 AGCGCT AGC^GCT
Eco81I 4925 CCTGAGG CC^T[ACGT]AGG
EcoO65I 84 GGTCACC G^GT[ACGT]ACC
EcoRI 1372 GAATTC G^AATTC
EcoT22I 1298 ATGCAT ATGCA^T
FbaI 320 TGATCA T^GATCA
HincII 484 GTCAAC GT[CT]^[GA]AC
HindII 484 GTCAAC GT[CT]^[GA]AC
HindIII 4589 AAGCTT A^AGCTT
KpnI 1145 GGTACC GGTAC^C
NdeI 4295 CATATG CA^TATG
NsiI 1298 ATGCAT ATGCA^T
PaeR7I 2875 CTCGAG C^TCGAG
RcaI 5021 TCATGA T^CATGA
SbfI 1494 CCTGCAGG CCTGCA^GG
SexAI 5015 ACCTGGT A^CC[AT]GGT
SgrAI 342 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2675 GCATGC GCATG^C
Sse8387I 1494 CCTGCAGG CCTGCA^GG
SspBI 698 TGTACA T^GTACA
XbaI 2582 TCTAGA T^CTAGA
XhoI 2875 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 1372 GAATTC [GA]^AATT[CT]
3852 AAATTC
AcyI 346 GGCGTC G[GA]^CG[CT]C
2151 GACGCC
ApoI 1372 GAATTC [GA]^AATT[CT]
3852 AAATTC
BbrPI 279 CACGTG CAC^GTG
4240 CACGTG
BbsI 705 GAAGAC GAAGAC(2/6)
3752 GTCTTC
BpuAI 705 GAAGAC GAAGAC(2/6)
3752 GTCTTC
BsaHI 346 GGCGTC G[GA]^CG[CT]C
2151 GACGCC
BsgI 2723 CTGCAC GTGCAG(16/14)
4180 CTGCAC
BsrDI 2415 CATTGC GCAATG(2/0)
3060 CATTGC
BstAPI 123 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2541 GCAGCTTCTGC
DraIII 1427 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1743 CACCCGGTG
DrdI 2916 GACAGTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3743 GACCCACTGGTC
EagI 807 CGGCCG C^GGCCG
2406 CGGCCG
EclXI 807 CGGCCG C^GGCCG
2406 CGGCCG
Eco52I 807 CGGCCG C^GGCCG
2406 CGGCCG
FseI 1363 GGCCGGCC GGCCGG^CC
1461 GGCCGGCC
HgaI 2151 GACGC GACGC(5/10)
347 GCGTC
Hin1I 346 GGCGTC G[GA]^CG[CT]C
2151 GACGCC
NcoI 1128 CCATGG C^CATGG
2057 CCATGG
PmaCI 279 CACGTG CAC^GTG
4240 CACGTG
PmlI 279 CACGTG CAC^GTG
4240 CACGTG
SfiI 799 GGCCCTGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1057 GGCCCCGGTGGCC
TfiI 419 GAATC G^A[AT]TC
625 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcgI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BsaAI BsaI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI