##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
AseI AsnI Asp718I AspI AviII
AvrII BbeI BclI BlnI BsaBI
BsiWI BsmBI Bsp1407I BspDI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII EcoRI EcoRV FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NarI NdeI NotI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 987 GTCGAC GT^[AC][GT]AC
AflII 3405 CTTAAG C^TTAAG
AocI 2949 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3704 AGCGCT AGC^GCT
Asp700I 2493 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1292 GGATCC G^GATCC
BbrPI 3004 CACGTG CAC^GTG
BcgI 87 CGACTACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3405 CTTAAG C^TTAAG
BglI 722 GCCTGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1553 AGATCT A^GATCT
BlpI 3635 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3635 GCTGAGC GC^T[ACGT]AGC
BsrDI 17 GCAATG GCAATG(2/0)
BstEII 3815 GGTCACC G^GT[ACGT]ACC
BstPI 3815 GGTCACC G^GT[ACGT]ACC
Bsu36I 2949 CCTGAGG CC^T[ACGT]AGG
CelII 3635 GCTGAGC GC^T[ACGT]AGC
DrdI 658 GACGGGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3704 AGCGCT AGC^GCT
Eco81I 2949 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3815 GGTCACC G^GT[ACGT]ACC
EcoT22I 4673 ATGCAT ATGCA^T
HincII 987 GTCGAC GT[CT]^[GA]AC
HindII 987 GTCGAC GT[CT]^[GA]AC
NaeI 1256 GCCGGC GCC^GGC
NgoMIV 1256 GCCGGC G^CCGGC
NheI 4919 GCTAGC G^CTAGC
NsiI 4673 ATGCAT ATGCA^T
PmaCI 3004 CACGTG CAC^GTG
PmlI 3004 CACGTG CAC^GTG
PshAI 1045 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 987 GTCGAC G^TCGAC
SapI 1461 GCTCTTC GCTCTTC(1/4)
SexAI 104 ACCAGGT A^CC[AT]GGT
XbaI 4015 TCTAGA T^CTAGA
XmnI 2493 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 1262 TCCGGA T^CCGGA
3364 TCCGGA
AcsI 4769 GAATTT [GA]^AATT[CT]
5381 AAATTT
AcyI 278 GACGCC G[GA]^CG[CT]C
2515 GACGCC
Alw44I 1663 GTGCAC G^TGCAC
4161 GTGCAC
ApaLI 1663 GTGCAC G^TGCAC
4161 GTGCAC
ApoI 4769 GAATTT [GA]^AATT[CT]
5381 AAATTT
BciVI 233 GTATCC GTATCC(6/5)
4731 GTATCC
BsaAI 520 TACGTG [CT]AC^GT[GA]
3004 CACGTG
BsaHI 278 GACGCC G[GA]^CG[CT]C
2515 GACGCC
BsaWI 1262 TCCGGA [AT]^CCGG[AT]
3364 TCCGGA
BseAI 1262 TCCGGA T^CCGGA
3364 TCCGGA
BsiEI 1254 CGGCCG CG[GA][CT]^CG
2184 CGGCCG
BsmI 3919 GAATGC GAATGC(1/-1)
4238 GAATGC
BspEI 1262 TCCGGA T^CCGGA
3364 TCCGGA
BspHI 2255 TCATGA T^CATGA
2402 TCATGA
BssHII 4357 GCGCGC G^CGCGC
4359 GCGCGC
DraI 3291 TTTAAA TTT^AAA
5360 TTTAAA
EagI 1254 CGGCCG C^GGCCG
2184 CGGCCG
EclXI 1254 CGGCCG C^GGCCG
2184 CGGCCG
Eco52I 1254 CGGCCG C^GGCCG
2184 CGGCCG
Hin1I 278 GACGCC G[GA]^CG[CT]C
2515 GACGCC
MroI 1262 TCCGGA T^CCGGA
3364 TCCGGA
PaeR7I 1 CTCGAG C^TCGAG
530 CTCGAG
RcaI 2255 TCATGA T^CATGA
2402 TCATGA
XhoI 1 CTCGAG C^TCGAG
530 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AfaI AflIII AhdI
AluI AlwI AlwNI ApaI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmaI SmlI SphI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI