##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbeI BciVI
BlnI BsaBI BseAI BspDI BspEI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI Eco47III
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI KpnI KspI MamI
MfeI MroI MunI NarI NheI
NotI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 2141 GACGTC GACGT^C
AccI 1170 GTCTAC GT^[AC][GT]AC
AflII 4546 CTTAAG C^TTAAG
AhdI 1889 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3471 GTGCAC G^TGCAC
AocI 2088 CCTGAGG CC^T[ACGT]AGG
ApaLI 3471 GTGCAC G^TGCAC
AspEI 1889 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2847 CACGTG CAC^GTG
BclI 2207 TGATCA T^GATCA
BfrI 4546 CTTAAG C^TTAAG
BglI 3000 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2847 CACGTG [CT]AC^GT[GA]
BsaWI 505 ACCGGA [AT]^CCGG[AT]
BsiEI 1034 CGGCCG CG[GA][CT]^CG
BsmBI 175 GAGACG CGTCTC(1/5)
BsmI 2703 GAATGC GAATGC(1/-1)
BsrDI 3166 CATTGC GCAATG(2/0)
BsrFI 3129 GCCGGC [GA]^CCGG[CT]
BstAPI 4273 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2088 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3129 GCCGGC [GA]^CCGG[CT]
EagI 1034 CGGCCG C^GGCCG
Eam1105I 1889 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1034 CGGCCG C^GGCCG
Eco52I 1034 CGGCCG C^GGCCG
Eco81I 2088 CCTGAGG CC^T[ACGT]AGG
FbaI 2207 TGATCA T^GATCA
MluI 3035 ACGCGT A^CGCGT
NaeI 3129 GCCGGC GCC^GGC
NdeI 3842 CATATG CA^TATG
NgoMIV 3129 GCCGGC G^CCGGC
NruI 3889 TCGCGA TCG^CGA
PaeR7I 2168 CTCGAG C^TCGAG
PmaCI 2847 CACGTG CAC^GTG
PmlI 2847 CACGTG CAC^GTG
SpeI 2923 ACTAGT A^CTAGT
StuI 68 AGGCCT AGG^CCT
XbaI 3704 TCTAGA T^CTAGA
XhoI 2168 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 177 GACGCC G[GA]^CG[CT]C
2141 GACGTC
AflIII 2848 ACGTGT A^C[GA][CT]GT
3035 ACGCGT
BalI 223 TGGCCA TGG^CCA
1561 TGGCCA
BanI 2665 GGCACC G^G[CT][GA]CC
4632 GGTGCC
BcgI 34 CGATCACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5313 CGACGACTTTGC
BglII 4683 AGATCT A^GATCT
4714 AGATCT
BlpI 1304 GCTCAGC GC^T[ACGT]AGC
1486 GCTCAGC
Bpu1102I 1304 GCTCAGC GC^T[ACGT]AGC
1486 GCTCAGC
BsaHI 177 GACGCC G[GA]^CG[CT]C
2141 GACGTC
BsiWI 650 CGTACG C^GTACG
3023 CGTACG
BspHI 1015 TCATGA T^CATGA
1231 TCATGA
BsrBI 1049 CCGCTC CCGCTC(-3/-3)
1806 GAGCGG
BssSI 3752 CACGAG CACGAG(-5/-1)
3688 CTCGTG
BstEII 755 GGTCACC G^GT[ACGT]ACC
3835 GGTCACC
BstPI 755 GGTCACC G^GT[ACGT]ACC
3835 GGTCACC
BstXI 2846 CCACGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3244 CCACTGCGCTGG
CelII 1304 GCTCAGC GC^T[ACGT]AGC
1486 GCTCAGC
DraIII 889 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
1613 CACTCGGTG
EcoO65I 755 GGTCACC G^GT[ACGT]ACC
3835 GGTCACC
EcoRI 423 GAATTC G^AATTC
2023 GAATTC
Hin1I 177 GACGCC G[GA]^CG[CT]C
2141 GACGTC
HincII 2955 GTCAAC GT[CT]^[GA]AC
3859 GTTGAC
HindII 2955 GTCAAC GT[CT]^[GA]AC
3859 GTTGAC
MluNI 223 TGGCCA TGG^CCA
1561 TGGCCA
MscI 223 TGGCCA TGG^CCA
1561 TGGCCA
RcaI 1015 TCATGA T^CATGA
1231 TCATGA
SapI 2618 GAAGAGC GCTCTTC(1/4)
839 GCTCTTC
ScaI 2906 AGTACT AGT^ACT
2920 AGTACT
SmaI 2573 CCCGGG CCC^GGG
3487 CCCGGG
SphI 3205 GCATGC GCATG^C
5177 GCATGC
XmaCI 2573 CCCGGG C^CCGGG
3487 CCCGGG
XmaI 2573 CCCGGG C^CCGGG
3487 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI Asp700I
AspHI AvaI AvaII BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI