##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AlwNI AocI
Aor51HI AscI Asp718I AviII AvrII
BamHI BbeI BbrPI BcgI BlnI
BseAI BsiWI BspDI BspEI BspHI
BsrBI BssSI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRV FseI FspI HaeII HindIII
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SnaBI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 1744 GTAGAC GT^[AC][GT]AC
AclI 2210 AACGTT AA^CGTT
AcyI 5 GACGCC G[GA]^CG[CT]C
AflII 4408 CTTAAG C^TTAAG
AgeI 1068 ACCGGT A^CCGGT
ApaI 4730 GGGCCC GGGCC^C
AseI 583 ATTAAT AT^TAAT
AsnI 583 ATTAAT AT^TAAT
BciVI 1648 GTATCC GTATCC(6/5)
BclI 960 TGATCA T^GATCA
BfrI 4408 CTTAAG C^TTAAG
BlpI 3032 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3032 GCTTAGC GC^T[ACGT]AGC
BsaAI 4969 TACGTG [CT]AC^GT[GA]
BsaBI 1251 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5 GACGCC G[GA]^CG[CT]C
BsaWI 1068 ACCGGT [AT]^CCGG[AT]
BseRI 1109 CTCCTC GAGGAG(10/8)
BsiEI 1468 CGACCG CG[GA][CT]^CG
BsmBI 3 GAGACG CGTCTC(1/5)
BspLU11I 2372 ACATGT A^CATGT
BsrFI 1068 ACCGGT [GA]^CCGG[CT]
BstAPI 2091 GCACATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 3032 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1068 ACCGGT [GA]^CCGG[CT]
DrdI 326 GACAGGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 37 GCGGG CCCGC(4/6)
FbaI 960 TGATCA T^GATCA
HgaI 5 GACGC GACGC(5/10)
Hin1I 5 GACGCC G[GA]^CG[CT]C
MamI 1251 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2171 CAATTG C^AATTG
MspA1I 2390 CAGCTG C[AC]G^C[GT]G
MunI 2171 CAATTG C^AATTG
NdeI 2530 CATATG CA^TATG
PinAI 1068 ACCGGT A^CCGGT
PpuMI 757 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 583 ATTAAT AT^TAAT
Psp1406I 2210 AACGTT AA^CGTT
PvuII 2390 CAGCTG CAG^CTG
SacI 3317 GAGCTC GAGCT^C
SapI 4208 GAAGAGC GCTCTTC(1/4)
ScaI 2366 AGTACT AGT^ACT
SgrAI 1067 CACCGGTG C[GA]^CCGG[CT]G
SmaI 3179 CCCGGG CCC^GGG
SpeI 3966 ACTAGT A^CTAGT
SphI 145 GCATGC GCATG^C
StuI 4717 AGGCCT AGG^CCT
XbaI 4423 TCTAGA T^CTAGA
XcmI 1464 CCATCGACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3179 CCCGGG C^CCGGG
XmaI 3179 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 2372 ACATGT A^C[GA][CT]GT
4970 ACGTGT
AhdI 3436 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3724 GACATTTGGTC
Alw44I 3232 GTGCAC G^TGCAC
4476 GTGCAC
ApaLI 3232 GTGCAC G^TGCAC
4476 GTGCAC
AspEI 3436 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3724 GACATTTGGTC
AspI 64 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
153 GACACTGTC
AvaI 3179 CCCGGG C^[CT]CG[GA]G
3211 CCCGAG
BanI 1626 GGCACC G^G[CT][GA]CC
1915 GGTGCC
BbsI 2309 GAAGAC GAAGAC(2/6)
4841 GAAGAC
BglI 801 GCCTACTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4645 GCCATGGAGGC
BglII 1182 AGATCT A^GATCT
1791 AGATCT
BpuAI 2309 GAAGAC GAAGAC(2/6)
4841 GAAGAC
BsaI 62 GAGACC GGTCTC(1/5)
1413 GGTCTC
BsoBI 3179 CCCGGG C^[CT]CG[GA]G
3211 CCCGAG
BsrDI 2933 CATTGC GCAATG(2/0)
4471 GCAATG
BssHII 4110 GCGCGC G^CGCGC
4112 GCGCGC
BstEII 398 GGTTACC G^GT[ACGT]ACC
4747 GGTTACC
BstPI 398 GGTTACC G^GT[ACGT]ACC
4747 GGTTACC
BstXI 1941 CCAACACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4269 CCAGCCCCTTGG
DraII 757 GGGTCCT [GA]G^G[ACGT]CC[CT]
4730 GGGCCCT
DsaI 4175 CCATGG C^C[GA][CT]GG
4646 CCATGG
Eam1105I 3436 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3724 GACATTTGGTC
EcoO109I 757 GGGTCCT [GA]G^G[ACGT]CC[CT]
4730 GGGCCCT
EcoO65I 398 GGTTACC G^GT[ACGT]ACC
4747 GGTTACC
EcoRI 681 GAATTC G^AATTC
2739 GAATTC
NcoI 4175 CCATGG C^CATGG
4646 CCATGG
PflFI 64 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
153 GACACTGTC
PflMI 1014 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2005 CCACCCCCTGG
PleI 3113 GAGTC GAGTC(4/5)
4768 GAGTC
PstI 2640 CTGCAG CTGCA^G
4361 CTGCAG
SexAI 1814 ACCAGGT A^CC[AT]GGT
4751 ACCTGGT
Tth111I 64 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
153 GACACTGTC
Van91I 1014 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2005 CCACCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BalI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI