##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccI AclI AflII AscI AseI
AsnI Asp700I AvrII BamHI BbeI
BcgI BclI BfrI BlnI BsaBI
BsiWI BspDI BspLU11I BssHII Bst1107I
BstZ17I ClaI CpoI DraIII EcoRV
FbaI HpaI KasI KspI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI PacI PaeR7I
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfoI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 3345 GACGTC GACGT^C
Acc65I 2883 GGTACC G^GTACC
AccII 3483 CGCG CG^CG
AflIII 2720 ACGTGT A^C[GA][CT]GT
AgeI 4108 ACCGGT A^CCGGT
ApaI 2842 GGGCCC GGGCC^C
Asp718I 2883 GGTACC G^GTACC
AspI 2749 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3570 CACGTG CAC^GTG
BciVI 4685 GGATAC GTATCC(6/5)
BglI 2844 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1462 AGATCT A^GATCT
BsaAI 3570 CACGTG [CT]AC^GT[GA]
BsiEI 2502 CGGCCG CG[GA][CT]^CG
BspHI 4356 TCATGA T^CATGA
BsrDI 4935 CATTGC GCAATG(2/0)
BstAPI 4805 GCATTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3912 TTCGAA TT^CGAA
BstUI 3483 CGCG CG^CG
EagI 2502 CGGCCG C^GGCCG
EclXI 2502 CGGCCG C^GGCCG
Eco52I 2502 CGGCCG C^GGCCG
EcoRI 3451 GAATTC G^AATTC
EcoT22I 5045 ATGCAT ATGCA^T
FseI 2499 GGCCGGCC GGCCGG^CC
KpnI 2883 GGTACC GGTAC^C
MvnI 3483 CGCG CG^CG
NaeI 2500 GCCGGC GCC^GGC
NgoMIV 2500 GCCGGC G^CCGGC
NsiI 5045 ATGCAT ATGCA^T
NspV 3912 TTCGAA TT^CGAA
PflFI 2749 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4108 ACCGGT A^CCGGT
PmaCI 3570 CACGTG CAC^GTG
PmlI 3570 CACGTG CAC^GTG
RcaI 4356 TCATGA T^CATGA
SexAI 1445 ACCAGGT A^CC[AT]GGT
SfiI 2843 GGCCCCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3912 TTCGAA TT^CGAA
SgrAI 2336 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 2847 CCCGGG CCC^GGG
SmlI 571 CTTGAG C^T[CT][GA]AG
SphI 2996 GCATGC GCATG^C
StuI 2877 AGGCCT AGG^CCT
Tth111I 2749 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2847 CCCGGG C^CCGGG
XmaI 2847 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 3451 GAATTC [GA]^AATT[CT]
3908 GAATTT
AcyI 1671 GACGCC G[GA]^CG[CT]C
3345 GACGTC
AhdI 3273 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3956 GACCCAGAGTC
Alw44I 2616 GTGCAC G^TGCAC
3158 GTGCAC
Aor51HI 754 AGCGCT AGC^GCT
4152 AGCGCT
ApaLI 2616 GTGCAC G^TGCAC
3158 GTGCAC
ApoI 3451 GAATTC [GA]^AATT[CT]
3908 GAATTT
AspEI 3273 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3956 GACCCAGAGTC
BanI 1678 GGCACC G^G[CT][GA]CC
2883 GGTACC
BbsI 918 GAAGAC GAAGAC(2/6)
1032 GAAGAC
BpuAI 918 GAAGAC GAAGAC(2/6)
1032 GAAGAC
BsaHI 1671 GACGCC G[GA]^CG[CT]C
3345 GACGTC
Bsp1407I 3256 TGTACA T^GTACA
4540 TGTACA
BsrGI 3256 TGTACA T^GTACA
4540 TGTACA
DrdI 2715 GACCGACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2719 GACGTGTCCGTC
Eam1105I 3273 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3956 GACCCAGAGTC
Eco47III 754 AGCGCT AGC^GCT
4152 AGCGCT
Hin1I 1671 GACGCC G[GA]^CG[CT]C
3345 GACGTC
HindIII 1112 AAGCTT A^AGCTT
4040 AAGCTT
PstI 1372 CTGCAG CTGCA^G
2938 CTGCAG
SacI 159 GAGCTC GAGCT^C
2260 GAGCTC
SspBI 3256 TGTACA T^GTACA
4540 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
AviII BalI BanII BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssKI BssSI BstEII BstF5I
BstNI BstPI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII