##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
Acc65I AclI AflII AocI AscI
AseI AsnI Asp718I AviII BciVI
BfrI BsiWI BspDI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI Eco81I
EcoRV EcoT22I FspI HpaI KpnI
KspI MluI NheI NotI NruI
NsiI NspV PacI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SfuI SnaBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 3763 GACGTC GACGT^C
AccI 1342 GTCTAC GT^[AC][GT]AC
AccIII 5674 TCCGGA T^CCGGA
AflIII 5849 ACATGT A^C[GA][CT]GT
AhdI 5442 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 5442 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 5529 GGCGCC GGCGC^C
BbrPI 2034 CACGTG CAC^GTG
BsaAI 2034 CACGTG [CT]AC^GT[GA]
BseAI 5674 TCCGGA T^CCGGA
BsmBI 4462 GAGACG CGTCTC(1/5)
BspEI 5674 TCCGGA T^CCGGA
BspLU11I 5849 ACATGT A^CATGT
BssHII 6301 GCGCGC G^CGCGC
BstEII 4846 GGTTACC G^GT[ACGT]ACC
BstPI 4846 GGTTACC G^GT[ACGT]ACC
DrdI 526 GACCAGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5442 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4846 GGTTACC G^GT[ACGT]ACC
EcoRI 6856 GAATTC G^AATTC
FseI 5737 GGCCGGCC GGCCGG^CC
HincII 7046 GTCAAC GT[CT]^[GA]AC
HindII 7046 GTCAAC GT[CT]^[GA]AC
HindIII 6163 AAGCTT A^AGCTT
KasI 5529 GGCGCC G^GCGCC
MroI 5674 TCCGGA T^CCGGA
NarI 5529 GGCGCC GG^CGCC
NdeI 1202 CATATG CA^TATG
PaeR7I 4798 CTCGAG C^TCGAG
PmaCI 2034 CACGTG CAC^GTG
PmlI 2034 CACGTG CAC^GTG
SapI 1387 GAAGAGC GCTCTTC(1/4)
SfoI 5529 GGCGCC GGC^GCC
SgrAI 4872 CACCGGTG C[GA]^CCGG[CT]G
SpeI 2637 ACTAGT A^CTAGT
SspI 1640 AATATT AAT^ATT
XhoI 4798 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AgeI 4873 ACCGGT A^CCGGT
5006 ACCGGT
Alw44I 4714 GTGCAC G^TGCAC
5888 GTGCAC
ApaLI 4714 GTGCAC G^TGCAC
5888 GTGCAC
Asp700I 6015 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6060 GAAGATCTTC
AspI 2163 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3841 GACGGGGTC
BamHI 1419 GGATCC G^GATCC
5406 GGATCC
BclI 2601 TGATCA T^GATCA
6706 TGATCA
BsaBI 3933 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5703 GATGTCCATC
DraIII 4174 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
4516 CACCAGGTG
EagI 3413 CGGCCG C^GGCCG
3452 CGGCCG
EclXI 3413 CGGCCG C^GGCCG
3452 CGGCCG
Eco52I 3413 CGGCCG C^GGCCG
3452 CGGCCG
FbaI 2601 TGATCA T^GATCA
6706 TGATCA
MamI 3933 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5703 GATGTCCATC
MfeI 1513 CAATTG C^AATTG
7114 CAATTG
MunI 1513 CAATTG C^AATTG
7114 CAATTG
PflFI 2163 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3841 GACGGGGTC
PinAI 4873 ACCGGT A^CCGGT
5006 ACCGGT
SbfI 3735 CCTGCAGG CCTGCA^GG
5074 CCTGCAGG
ScaI 899 AGTACT AGT^ACT
7202 AGTACT
SfiI 621 GGCCTACCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3172 GGCCCCGTGGGCC
SphI 272 GCATGC GCATG^C
1670 GCATGC
Sse8387I 3735 CCTGCAGG CCTGCA^GG
5074 CCTGCAGG
Tth111I 2163 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3841 GACGGGGTC
XmnI 6015 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6060 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApaI
ApoI AspHI AvaI AvaII AvrII
BalI BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmaI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI