##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccIII AgeI AhdI AocI
Aor51HI ApaI AscI Asp718I AspEI
AspI AvrII BbeI BbrPI BbvCI
BcgI BlnI BsaAI BsaI BseAI
BsiEI BsiWI BspDI BspEI BstBI
Bsu36I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV FseI HpaI KasI
KpnI MluI MroI NarI NotI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SalI SapI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SwaI Tth111I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1815 GACGTC GACGT^C
AclI 1144 AACGTT AA^CGTT
AflII 3000 CTTAAG C^TTAAG
AviII 1894 TGCGCA TGC^GCA
BamHI 2260 GGATCC G^GATCC
BclI 3128 TGATCA T^GATCA
BfrI 3000 CTTAAG C^TTAAG
BlpI 4485 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4485 GCTTAGC GC^T[ACGT]AGC
BsaBI 2639 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2171 TCCGGT [AT]^CCGG[AT]
BsmI 3189 GAATGC GAATGC(1/-1)
BsrFI 1046 GCCGGC [GA]^CCGG[CT]
BssHII 82 GCGCGC G^CGCGC
BstAPI 133 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 913 GGTGACC G^GT[ACGT]ACC
BstPI 913 GGTGACC G^GT[ACGT]ACC
BstXI 1504 CCAGCACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4485 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1046 GCCGGC [GA]^CCGG[CT]
DrdI 2448 GACGAACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 913 GGTGACC G^GT[ACGT]ACC
FbaI 3128 TGATCA T^GATCA
FspI 1894 TGCGCA TGC^GCA
HincII 3974 GTTGAC GT[CT]^[GA]AC
HindII 3974 GTTGAC GT[CT]^[GA]AC
KspI 105 CCGCGG CCGC^GG
MamI 2639 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1046 GCCGGC GCC^GGC
NgoMIV 1046 GCCGGC G^CCGGC
NheI 2016 GCTAGC G^CTAGC
PpuMI 1074 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1144 AACGTT AA^CGTT
SacI 176 GAGCTC GAGCT^C
SacII 105 CCGCGG CCGC^GG
SpeI 865 ACTAGT A^CTAGT
StuI 1889 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 927 GACGCC G[GA]^CG[CT]C
1815 GACGTC
AflIII 4400 ACATGT A^C[GA][CT]GT
5311 ACATGT
Alw44I 42 GTGCAC G^TGCAC
3430 GTGCAC
ApaLI 42 GTGCAC G^TGCAC
3430 GTGCAC
BanI 1110 GGCACC G^G[CT][GA]CC
2093 GGTGCC
BciVI 1421 GGATAC GTATCC(6/5)
2181 GTATCC
BglI 145 GCCGCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
148 GCCACGGCGGC
BglII 4132 AGATCT A^GATCT
4661 AGATCT
BsaHI 927 GACGCC G[GA]^CG[CT]C
1815 GACGTC
BsmFI 1180 GGGAC GGGAC(10/14)
4069 GGGAC
BspHI 1675 TCATGA T^CATGA
1747 TCATGA
BspLU11I 4400 ACATGT A^CATGT
5311 ACATGT
BsrBI 111 GAGCGG CCGCTC(-3/-3)
1632 GAGCGG
BssSI 1290 CACGAG CACGAG(-5/-1)
2436 CACGAG
Bst1107I 2051 GTATAC GTA^TAC
2620 GTATAC
BstZ17I 2051 GTATAC GTA^TAC
2620 GTATAC
EcoT14I 1960 CCATGG C^C[AT][AT]GG
3461 CCATGG
EcoT22I 3333 ATGCAT ATGCA^T
4111 ATGCAT
Hin1I 927 GACGCC G[GA]^CG[CT]C
1815 GACGTC
HindIII 2989 AAGCTT A^AGCTT
4483 AAGCTT
MfeI 2861 CAATTG C^AATTG
4988 CAATTG
MunI 2861 CAATTG C^AATTG
4988 CAATTG
NcoI 1960 CCATGG C^CATGG
3461 CCATGG
NdeI 3277 CATATG CA^TATG
5183 CATATG
NruI 8 TCGCGA TCG^CGA
1564 TCGCGA
NsiI 3333 ATGCAT ATGCA^T
4111 ATGCAT
PvuII 371 CAGCTG CAG^CTG
2331 CAGCTG
RcaI 1675 TCATGA T^CATGA
1747 TCATGA
SbfI 693 CCTGCAGG CCTGCA^GG
1406 CCTGCAGG
ScaI 868 AGTACT AGT^ACT
2818 AGTACT
SmlI 1885 CTCAAG C^T[CT][GA]AG
3000 CTTAAG
Sse8387I 693 CCTGCAGG CCTGCA^GG
1406 CCTGCAGG
StyI 1960 CCATGG C^C[AT][AT]GG
3461 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
BalI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PshBI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SphI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XhoII XmnI