##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AgeI AscI
AseI AsnI Asp700I Asp718I AspI
AviII BamHI BbeI BbrPI BclI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraIII DrdI
EcoRI FbaI FspI HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NarI NdeI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI PvuI
RcaI RsrII SalI SbfI SexAI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI Tth111I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2295 GTAGAC GT^[AC][GT]AC
AclI 3829 AACGTT AA^CGTT
AcyI 3741 GACGCC G[GA]^CG[CT]C
Alw44I 1566 GTGCAC G^TGCAC
Aor51HI 5642 AGCGCT AGC^GCT
ApaLI 1566 GTGCAC G^TGCAC
BanI 4618 GGTGCC G^G[CT][GA]CC
BciVI 4243 GTATCC GTATCC(6/5)
BsaHI 3741 GACGCC G[GA]^CG[CT]C
BstEII 2040 GGTCACC G^GT[ACGT]ACC
BstPI 2040 GGTCACC G^GT[ACGT]ACC
DraI 1928 TTTAAA TTT^AAA
Eco47III 5642 AGCGCT AGC^GCT
EcoO65I 2040 GGTCACC G^GT[ACGT]ACC
EcoRV 4993 GATATC GAT^ATC
EcoT22I 1462 ATGCAT ATGCA^T
FseI 2660 GGCCGGCC GGCCGG^CC
HaeII 5642 AGCGCT [GA]GCGC^[CT]
Hin1I 3741 GACGCC G[GA]^CG[CT]C
HincII 464 GTCAAC GT[CT]^[GA]AC
HindII 464 GTCAAC GT[CT]^[GA]AC
HindIII 5205 AAGCTT A^AGCTT
KspI 23 CCGCGG CCGC^GG
NsiI 1462 ATGCAT ATGCA^T
PshAI 1626 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3829 AACGTT AA^CGTT
SacI 1775 GAGCTC GAGCT^C
SacII 23 CCGCGG CCGC^GG
ScaI 2611 AGTACT AGT^ACT
SpeI 1843 ACTAGT A^CTAGT
SspI 3907 AATATT AAT^ATT
SwaI 1927 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 2385 CTTAAG C^TTAAG
4442 CTTAAG
AocI 350 CCTGAGG CC^T[ACGT]AGG
5665 CCTGAGG
AvrII 2127 CCTAGG C^CTAGG
3633 CCTAGG
BbsI 4785 GAAGAC GAAGAC(2/6)
2782 GTCTTC
BcgI 5699 CGATTGCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
269 GCAGGAACCTCG
BfrI 2385 CTTAAG C^TTAAG
4442 CTTAAG
BlnI 2127 CCTAGG C^CTAGG
3633 CCTAGG
BlpI 4139 GCTGAGC GC^T[ACGT]AGC
5082 GCTCAGC
Bpu1102I 4139 GCTGAGC GC^T[ACGT]AGC
5082 GCTCAGC
BpuAI 4785 GAAGAC GAAGAC(2/6)
2782 GTCTTC
BsaI 695 GAGACC GGTCTC(1/5)
1666 GGTCTC
BsiEI 304 CGGCCG CG[GA][CT]^CG
2663 CGGCCG
BsmI 1460 GAATGC GAATGC(1/-1)
4061 GCATTC
BspLU11I 1562 ACATGT A^CATGT
2495 ACATGT
BsrFI 302 GCCGGC [GA]^CCGG[CT]
2661 GCCGGC
Bsu36I 350 CCTGAGG CC^T[ACGT]AGG
5665 CCTGAGG
CelII 4139 GCTGAGC GC^T[ACGT]AGC
5082 GCTCAGC
Cfr10I 302 GCCGGC [GA]^CCGG[CT]
2661 GCCGGC
EagI 304 CGGCCG C^GGCCG
2663 CGGCCG
EclXI 304 CGGCCG C^GGCCG
2663 CGGCCG
Eco52I 304 CGGCCG C^GGCCG
2663 CGGCCG
Eco81I 350 CCTGAGG CC^T[ACGT]AGG
5665 CCTGAGG
NaeI 302 GCCGGC GCC^GGC
2661 GCCGGC
NcoI 81 CCATGG C^CATGG
5377 CCATGG
NgoMIV 302 GCCGGC G^CCGGC
2661 GCCGGC
NheI 4377 GCTAGC G^CTAGC
5190 GCTAGC
SapI 815 GAAGAGC GCTCTTC(1/4)
3038 GCTCTTC
SfiI 2132 GGCCAAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4679 GGCCAGCCAGGCC
SphI 1762 GCATGC GCATG^C
5612 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AhdI AluI AlwI AlwNI ApaI
ApoI AspEI AspHI AvaI AvaII
BalI BanII BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII