##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AscI AspEI AviII BbrPI BseAI
BsiWI BspEI BssHII Bst1107I BstBI
BstZ17I CpoI DraIII DrdI Eam1105I
FseI FspI MluI MroI NheI
NotI NruI NspV PinAI PmaCI
PmlI Psp1406I PvuI RsrII SalI
SbfI ScaI SfiI SfuI SnaBI
SpeI Sse8387I SwaI XcmI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1615 GTCTAC GT^[AC][GT]AC
Aor51HI 465 AGCGCT AGC^GCT
ApaI 1278 GGGCCC GGGCC^C
Asp700I 2715 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1380 CCTAGG C^CTAGG
BamHI 4873 GGATCC G^GATCC
BbeI 1262 GGCGCC GGCGC^C
BlnI 1380 CCTAGG C^CTAGG
BlpI 1395 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1395 GCTGAGC GC^T[ACGT]AGC
BsaAI 3473 CACGTA [CT]AC^GT[GA]
BsaBI 3463 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 5838 GAGACC GGTCTC(1/5)
BsaWI 2097 TCCGGT [AT]^CCGG[AT]
Bsp1407I 1592 TGTACA T^GTACA
BspDI 1156 ATCGAT AT^CGAT
BsrBI 3952 CCGCTC CCGCTC(-3/-3)
BsrGI 1592 TGTACA T^GTACA
BssSI 671 CACGAG CACGAG(-5/-1)
BstXI 2771 CCATTTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1395 GCTGAGC GC^T[ACGT]AGC
ClaI 1156 ATCGAT AT^CGAT
EagI 430 CGGCCG C^GGCCG
EclXI 430 CGGCCG C^GGCCG
Eco47III 465 AGCGCT AGC^GCT
Eco52I 430 CGGCCG C^GGCCG
EcoT22I 3251 ATGCAT ATGCA^T
HpaI 4650 GTTAAC GTT^AAC
KasI 1262 GGCGCC G^GCGCC
KspI 235 CCGCGG CCGC^GG
MamI 3463 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1000 GCCGGC GCC^GGC
NarI 1262 GGCGCC GG^CGCC
NcoI 2777 CCATGG C^CATGG
NgoMIV 1000 GCCGGC G^CCGGC
NsiI 3251 ATGCAT ATGCA^T
PaeR7I 713 CTCGAG C^TCGAG
PmeI 3970 GTTTAAAC GTTT^AAAC
PshAI 968 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 235 CCGCGG CCGC^GG
SapI 1720 GCTCTTC GCTCTTC(1/4)
SfoI 1262 GGCGCC GGC^GCC
SgrAI 999 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 813 CCCGGG CCC^GGG
SphI 885 GCATGC GCATG^C
SspBI 1592 TGTACA T^GTACA
XbaI 3493 TCTAGA T^CTAGA
XhoI 713 CTCGAG C^TCGAG
XmaCI 813 CCCGGG C^CCGGG
XmaI 813 CCCGGG C^CCGGG
XmnI 2715 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 236 CGCG CG^CG
1980 CGCG
AcyI 567 GACGCC G[GA]^CG[CT]C
1262 GGCGCC
AflII 97 CTTAAG C^TTAAG
3140 CTTAAG
Alw44I 4561 GTGCAC G^TGCAC
5299 GTGCAC
AocI 206 CCTGAGG CC^T[ACGT]AGG
2284 CCTGAGG
ApaLI 4561 GTGCAC G^TGCAC
5299 GTGCAC
AvaI 713 CTCGAG C^[CT]CG[GA]G
813 CCCGGG
BalI 774 TGGCCA TGG^CCA
1234 TGGCCA
BbvCI 450 CCTCAGC CCTCAGC(-5/-2)
679 CCTCAGC
BcgI 1158 CGATTCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1191 CGAATATGTTGC
BfrI 97 CTTAAG C^TTAAG
3140 CTTAAG
BglI 440 GCCCGTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2985 GCCATGATGGC
BglII 354 AGATCT A^GATCT
5106 AGATCT
BsaHI 567 GACGCC G[GA]^CG[CT]C
1262 GGCGCC
BsiEI 430 CGGCCG CG[GA][CT]^CG
1043 CGACCG
BsmBI 2027 GAGACG CGTCTC(1/5)
2605 GAGACG
BsmI 5524 GAATGC GAATGC(1/-1)
753 GCATTC
BsoBI 713 CTCGAG C^[CT]CG[GA]G
813 CCCGGG
BstEII 3016 GGTAACC G^GT[ACGT]ACC
3446 GGTAACC
BstPI 3016 GGTAACC G^GT[ACGT]ACC
3446 GGTAACC
BstUI 236 CGCG CG^CG
1980 CGCG
Bsu36I 206 CCTGAGG CC^T[ACGT]AGG
2284 CCTGAGG
DsaI 235 CCGCGG C^C[GA][CT]GG
2777 CCATGG
Eco81I 206 CCTGAGG CC^T[ACGT]AGG
2284 CCTGAGG
EcoO65I 3016 GGTAACC G^GT[ACGT]ACC
3446 GGTAACC
EcoRV 4951 GATATC GAT^ATC
5741 GATATC
Hin1I 567 GACGCC G[GA]^CG[CT]C
1262 GGCGCC
MluNI 774 TGGCCA TGG^CCA
1234 TGGCCA
MscI 774 TGGCCA TGG^CCA
1234 TGGCCA
MvnI 236 CGCG CG^CG
1980 CGCG
NdeI 1973 CATATG CA^TATG
3248 CATATG
PacI 5050 TTAATTAA TTAAT^TAA
5084 TTAATTAA
PvuII 249 CAGCTG CAG^CTG
3208 CAGCTG
SexAI 849 ACCAGGT A^CC[AT]GGT
3012 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp718I AspHI AspI AvaII
BanI BanII BbsI BbvI BciVI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspLU11I BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PshBI
PstI RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XhoII