##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AvrII BfrI BlnI BsaBI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrDI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoRV FseI HindIII
HpaI KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 879 GACGTC GACGT^C
Acc65I 1761 GGTACC G^GTACC
AccII 501 CGCG CG^CG
Asp700I 3439 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1761 GGTACC G^GTACC
AspI 1272 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2831 CACGTG CAC^GTG
BcgI 2566 CGAGCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 814 GGATAC GTATCC(6/5)
BclI 254 TGATCA T^GATCA
BglII 3538 AGATCT A^GATCT
BspHI 5507 TCATGA T^CATGA
BstUI 501 CGCG CG^CG
EcoT22I 120 ATGCAT ATGCA^T
FbaI 254 TGATCA T^GATCA
HincII 661 GTCAAC GT[CT]^[GA]AC
HindII 661 GTCAAC GT[CT]^[GA]AC
KpnI 1761 GGTACC GGTAC^C
MvnI 501 CGCG CG^CG
NaeI 3105 GCCGGC GCC^GGC
NgoMIV 3105 GCCGGC G^CCGGC
NsiI 120 ATGCAT ATGCA^T
PaeR7I 2168 CTCGAG C^TCGAG
PflFI 1272 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2831 CACGTG CAC^GTG
PmlI 2831 CACGTG CAC^GTG
PshAI 1220 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5507 TCATGA T^CATGA
ScaI 2164 AGTACT AGT^ACT
SexAI 1273 ACCTGGT A^CC[AT]GGT
Tth111I 1272 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2168 CTCGAG C^TCGAG
XmnI 3439 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AviII 3793 TGCGCA TGC^GCA
3843 TGCGCA
BamHI 2032 GGATCC G^GATCC
3995 GGATCC
BbeI 3102 GGCGCC GGCGC^C
3774 GGCGCC
BfaI 423 CTAG C^TAG
5632 CTAG
BglI 3179 GCCAGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5686 GCCGTTGTGGC
BsaAI 212 TACGTG [CT]AC^GT[GA]
2831 CACGTG
BsaWI 3014 ACCGGA [AT]^CCGG[AT]
4584 ACCGGA
BspLU11I 186 ACATGT A^CATGT
4488 ACATGT
BssSI 1790 CACGAG CACGAG(-5/-1)
2849 CACGAG
BstEII 1333 GGTGACC G^GT[ACGT]ACC
4620 GGTTACC
BstPI 1333 GGTGACC G^GT[ACGT]ACC
4620 GGTTACC
DraI 5254 TTTAAA TTT^AAA
5780 TTTAAA
EcoO65I 1333 GGTGACC G^GT[ACGT]ACC
4620 GGTTACC
FspI 3793 TGCGCA TGC^GCA
3843 TGCGCA
KasI 3102 GGCGCC G^GCGCC
3774 GGCGCC
MaeI 423 CTAG C^TAG
5632 CTAG
NarI 3102 GGCGCC GG^CGCC
3774 GGCGCC
NcoI 1576 CCATGG C^CATGG
2142 CCATGG
SfoI 3102 GGCGCC GGC^GCC
3774 GGCGCC
SmaI 450 CCCGGG CCC^GGG
4245 CCCGGG
StuI 1747 AGGCCT AGG^CCT
3969 AGGCCT
XmaCI 450 CCCGGG C^CCGGG
4245 CCCGGG
XmaI 450 CCCGGG C^CCGGG
4245 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AhdI AluI AlwI AlwNI AocI
ApaI ApoI AspEI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII