##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Aor51HI AscI AviII BalI BbeI
BciVI BsaBI BsaHI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BsrFI
BssHII Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI DrdI EaeI EagI
EclXI Eco47III Eco52I EcoRV FseI
FspI Hin1I HpaI KasI KspI
MamI MluI MluNI MroI MscI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2430 GGTACC G^GTACC
AseI 3112 ATTAAT AT^TAAT
AsnI 3112 ATTAAT AT^TAAT
Asp718I 2430 GGTACC G^GTACC
AspI 1224 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 487 GGATCC G^GATCC
BbrPI 1564 CACGTG CAC^GTG
BcgI 650 CGATGAATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2760 GCCTCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4507 AGATCT A^GATCT
BlpI 5908 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5908 GCTCAGC GC^T[ACGT]AGC
BsaAI 1564 CACGTG [CT]AC^GT[GA]
BsaWI 1255 ACCGGA [AT]^CCGG[AT]
BsmBI 4451 GAGACG CGTCTC(1/5)
BspLU11I 3424 ACATGT A^CATGT
BsrDI 661 CATTGC GCAATG(2/0)
BssSI 1383 CTCGTG CACGAG(-5/-1)
BstAPI 3511 GCAGCATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 5908 GCTCAGC GC^T[ACGT]AGC
DraIII 5802 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3807 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4346 GAATTC G^AATTC
HaeII 1084 GGCGCT [GA]GCGC^[CT]
HgaI 4453 GACGC GACGC(5/10)
KpnI 2430 GGTACC GGTAC^C
MfeI 571 CAATTG C^AATTG
MunI 571 CAATTG C^AATTG
NcoI 145 CCATGG C^CATGG
PflFI 1224 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1564 CACGTG CAC^GTG
PmlI 1564 CACGTG CAC^GTG
PshAI 3374 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3112 ATTAAT AT^TAAT
SexAI 5724 ACCTGGT A^CC[AT]GGT
Tth111I 1224 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2346 TCTAGA T^CTAGA
XcmI 5811 CCAAACTTAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2829 GTAGAC GT^[AC][GT]AC
5957 GTAGAC
AccII 4455 CGCG CG^CG
5202 CGCG
AflII 5286 CTTAAG C^TTAAG
5414 CTTAAG
AflIII 1563 ACACGT A^C[GA][CT]GT
3424 ACATGT
AhdI 959 GACGGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4522 GACTGTAAGTC
AlwNI 1431 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2151 CAGTATCTG
AocI 2619 CCTGAGG CC^T[ACGT]AGG
3023 CCTGAGG
AspEI 959 GACGGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4522 GACTGTAAGTC
AvaI 73 CCCGGG C^[CT]CG[GA]G
370 CCCGGG
AvrII 1721 CCTAGG C^CTAGG
4192 CCTAGG
BbvCI 4776 CCTCAGC CCTCAGC(-5/-2)
4912 CCTCAGC
BfrI 5286 CTTAAG C^TTAAG
5414 CTTAAG
BlnI 1721 CCTAGG C^CTAGG
4192 CCTAGG
BsaI 3787 GAGACC GGTCTC(1/5)
4878 GGTCTC
BsoBI 73 CCCGGG C^[CT]CG[GA]G
370 CCCGGG
BspHI 350 TCATGA T^CATGA
3783 TCATGA
BstEII 2023 GGTGACC G^GT[ACGT]ACC
3917 GGTCACC
BstPI 2023 GGTGACC G^GT[ACGT]ACC
3917 GGTCACC
BstUI 4455 CGCG CG^CG
5202 CGCG
Bsu36I 2619 CCTGAGG CC^T[ACGT]AGG
3023 CCTGAGG
Eam1105I 959 GACGGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4522 GACTGTAAGTC
Eco81I 2619 CCTGAGG CC^T[ACGT]AGG
3023 CCTGAGG
EcoO65I 2023 GGTGACC G^GT[ACGT]ACC
3917 GGTCACC
MaeII 1225 ACGT A^CGT
1565 ACGT
MvnI 4455 CGCG CG^CG
5202 CGCG
RcaI 350 TCATGA T^CATGA
3783 TCATGA
SacI 3070 GAGCTC GAGCT^C
3992 GAGCTC
SmaI 73 CCCGGG CCC^GGG
370 CCCGGG
SpeI 4584 ACTAGT A^CTAGT
5126 ACTAGT
SphI 1174 GCATGC GCATG^C
4806 GCATGC
XmaCI 73 CCCGGG C^CCGGG
370 CCCGGG
XmaI 73 CCCGGG C^CCGGG
370 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI ApaI ApaLI ApoI Asp700I
AspHI AvaII BanI BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI