##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AccIII AclI AflII AscI AseI
AsnI AspI BcgI BciVI BclI
BfrI BsaI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
MluI MroI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI SexAI SfiI SfuI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1887 GACGTC GACGT^C
Acc65I 387 GGTACC G^GTACC
AccI 350 GTCTAC GT^[AC][GT]AC
AgeI 4022 ACCGGT A^CCGGT
Alw44I 1764 GTGCAC G^TGCAC
Aor51HI 165 AGCGCT AGC^GCT
ApaLI 1764 GTGCAC G^TGCAC
Asp718I 387 GGTACC G^GTACC
AvaI 2303 CCCGGG C^[CT]CG[GA]G
AviII 2107 TGCGCA TGC^GCA
BamHI 4427 GGATCC G^GATCC
BbeI 1824 GGCGCC GGCGC^C
BbrPI 1050 CACGTG CAC^GTG
BglI 5238 GCCAATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3696 AGATCT A^GATCT
BsaBI 4416 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4022 ACCGGT [AT]^CCGG[AT]
BsoBI 2303 CCCGGG C^[CT]CG[GA]G
BsrDI 3313 GCAATG GCAATG(2/0)
BsrFI 4022 ACCGGT [GA]^CCGG[CT]
Cfr10I 4022 ACCGGT [GA]^CCGG[CT]
Eco47III 165 AGCGCT AGC^GCT
FspI 2107 TGCGCA TGC^GCA
KasI 1824 GGCGCC G^GCGCC
KpnI 387 GGTACC GGTAC^C
MamI 4416 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3228 CAATTG C^AATTG
MunI 3228 CAATTG C^AATTG
NarI 1824 GGCGCC GG^CGCC
PinAI 4022 ACCGGT A^CCGGT
PmaCI 1050 CACGTG CAC^GTG
PmlI 1050 CACGTG CAC^GTG
ScaI 2254 AGTACT AGT^ACT
SfoI 1824 GGCGCC GGC^GCC
SgrAI 4021 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2303 CCCGGG CCC^GGG
SphI 2870 GCATGC GCATG^C
TaqI 278 TCGA T^CGA
TthHB8I 278 TCGA T^CGA
XmaCI 2303 CCCGGG C^CCGGG
XmaI 2303 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AflIII 1675 ACATGT A^C[GA][CT]GT
3795 ACATGT
AvrII 2273 CCTAGG C^CTAGG
4160 CCTAGG
BlnI 2273 CCTAGG C^CTAGG
4160 CCTAGG
BsaAI 1050 CACGTG [CT]AC^GT[GA]
2361 CACGTA
BsmBI 138 CGTCTC CGTCTC(1/5)
3275 GAGACG
BspLU11I 1675 ACATGT A^CATGT
3795 ACATGT
BssSI 3381 CACGAG CACGAG(-5/-1)
1843 CTCGTG
HaeII 165 AGCGCT [GA]GCGC^[CT]
1824 GGCGCC
HgaI 151 GACGC GACGC(5/10)
3277 GACGC
HindIII 1223 AAGCTT A^AGCTT
2637 AAGCTT
KspI 271 CCGCGG CCGC^GG
2026 CCGCGG
NdeI 1932 CATATG CA^TATG
2115 CATATG
PflMI 4206 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4739 CCAGGTGATGG
SacII 271 CCGCGG CCGC^GG
2026 CCGCGG
Van91I 4206 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4739 CCAGGTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspEI AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PshAI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII XmnI