##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AclI AflII AscI AviII BbeI
BcgI BciVI BfrI BsaBI BsiEI
BsiWI BspDI BsrBI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI EagI
EclXI Eco52I EcoRI FseI FspI
HgaI HindIII HpaI KasI KspI
MamI MfeI MluI MunI NarI
NheI NotI NruI NspV PacI
PaeR7I PmeI Psp1406I PvuI RsrII
SacI SacII SfoI SfuI SgrAI
SmaI SnaBI SpeI SspI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 3499 GACGTC GACGT^C
AccIII 1081 TCCGGA T^CCGGA
AcyI 3499 GACGTC G[GA]^CG[CT]C
AgeI 1087 ACCGGT A^CCGGT
AhdI 3595 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1245 GTGCAC G^TGCAC
Aor51HI 1774 AGCGCT AGC^GCT
ApaI 2086 GGGCCC GGGCC^C
ApaLI 1245 GTGCAC G^TGCAC
AseI 4709 ATTAAT AT^TAAT
AsnI 4709 ATTAAT AT^TAAT
AspEI 3595 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2386 GGATCC G^GATCC
BbrPI 1689 CACGTG CAC^GTG
BcnI 2483 CCCGG CC^[GC]GG
BglI 4458 GCCAGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3671 AGATCT A^GATCT
BsaAI 1689 CACGTG [CT]AC^GT[GA]
BsaHI 3499 GACGTC G[GA]^CG[CT]C
BseAI 1081 TCCGGA T^CCGGA
BsmBI 4253 GAGACG CGTCTC(1/5)
BspEI 1081 TCCGGA T^CCGGA
BstAPI 4160 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eam1105I 3595 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1774 AGCGCT AGC^GCT
EcoT22I 3857 ATGCAT ATGCA^T
Hin1I 3499 GACGTC G[GA]^CG[CT]C
MroI 1081 TCCGGA T^CCGGA
NaeI 1184 GCCGGC GCC^GGC
NciI 2483 CCCGG CC^[GC]GG
NgoMIV 1184 GCCGGC G^CCGGC
NsiI 3857 ATGCAT ATGCA^T
PinAI 1087 ACCGGT A^CCGGT
PmaCI 1689 CACGTG CAC^GTG
PmlI 1689 CACGTG CAC^GTG
PshAI 548 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4709 ATTAAT AT^TAAT
SapI 836 GCTCTTC GCTCTTC(1/4)
SbfI 557 CCTGCAGG CCTGCA^GG
ScaI 4638 AGTACT AGT^ACT
SexAI 206 ACCAGGT A^CC[AT]GGT
SfiI 4457 GGCCAGACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 557 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1134 GTCGAC GT^[AC][GT]AC
2847 GTCGAC
AflIII 910 ACATGT A^C[GA][CT]GT
3433 ACATGT
AspHI 347 GAGCAC G[AT]GC[AT]^C
1245 GTGCAC
AvrII 3741 CCTAGG C^CTAGG
4318 CCTAGG
BalI 128 TGGCCA TGG^CCA
3515 TGGCCA
BclI 1273 TGATCA T^GATCA
3452 TGATCA
BlnI 3741 CCTAGG C^CTAGG
4318 CCTAGG
BsaWI 1081 TCCGGA [AT]^CCGG[AT]
1087 ACCGGT
BsiHKAI 347 GAGCAC G[AT]GC[AT]^C
1245 GTGCAC
Bsp1407I 472 TGTACA T^GTACA
2156 TGTACA
BspLU11I 910 ACATGT A^CATGT
3433 ACATGT
BsrDI 794 CATTGC GCAATG(2/0)
124 GCAATG
BsrGI 472 TGTACA T^GTACA
2156 TGTACA
BstEII 210 GGTGACC G^GT[ACGT]ACC
485 GGTGACC
BstPI 210 GGTGACC G^GT[ACGT]ACC
485 GGTGACC
DraI 3779 TTTAAA TTT^AAA
4126 TTTAAA
DrdI 2405 GACACCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3757 GACAGGTACGTC
EarI 837 CTCTTC CTCTTC(1/4)
4445 GAAGAG
EcoO65I 210 GGTGACC G^GT[ACGT]ACC
485 GGTGACC
EcoRV 825 GATATC GAT^ATC
4618 GATATC
FbaI 1273 TGATCA T^GATCA
3452 TGATCA
Ksp632I 837 CTCTTC CTCTTC(1/4)
4445 GAAGAG
MluNI 128 TGGCCA TGG^CCA
3515 TGGCCA
MscI 128 TGGCCA TGG^CCA
3515 TGGCCA
NdeI 2310 CATATG CA^TATG
3417 CATATG
SalI 1134 GTCGAC G^TCGAC
2847 GTCGAC
SphI 1870 GCATGC GCATG^C
4281 GCATGC
SspBI 472 TGTACA T^GTACA
2156 TGTACA
StuI 893 AGGCCT AGG^CCT
4308 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I Asp718I AspI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI KpnI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmnI