##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
AviII BamHI BciVI BfrI BglII
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspEI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstBI BstZ17I Cfr10I
CpoI DraI EagI EclXI Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NotI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I RsrII SalI SbfI
ScaI SfiI SfuI SgrAI SpeI
SphI Sse8387I SspBI SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 670 GTCTAC GT^[AC][GT]AC
AcyI 1426 GGCGCC G[GA]^CG[CT]C
AflIII 4098 ACACGT A^C[GA][CT]GT
AhdI 4312 GACTCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4312 GACTCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1426 GGCGCC GGCGC^C
BbrPI 4415 CACGTG CAC^GTG
BclI 536 TGATCA T^GATCA
BsaHI 1426 GGCGCC G[GA]^CG[CT]C
BsiEI 2046 CGATCG CG[GA][CT]^CG
BspDI 2044 ATCGAT AT^CGAT
BspHI 662 TCATGA T^CATGA
ClaI 2044 ATCGAT AT^CGAT
DraIII 4412 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2104 GACTGGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4312 GACTCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 536 TGATCA T^GATCA
Hin1I 1426 GGCGCC G[GA]^CG[CT]C
KasI 1426 GGCGCC G^GCGCC
KspI 2 CCGCGG CCGC^GG
NarI 1426 GGCGCC GG^CGCC
NruI 4303 TCGCGA TCG^CGA
PmaCI 4415 CACGTG CAC^GTG
PmlI 4415 CACGTG CAC^GTG
PvuI 2046 CGATCG CGAT^CG
RcaI 662 TCATGA T^CATGA
SacII 2 CCGCGG CCGC^GG
SexAI 1205 ACCAGGT A^CC[AT]GGT
SfoI 1426 GGCGCC GGC^GCC
SmaI 3759 CCCGGG CCC^GGG
SnaBI 4379 TACGTA TAC^GTA
XbaI 876 TCTAGA T^CTAGA
XcmI 332 CCACAGGTAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3759 CCCGGG C^CCGGG
XmaI 3759 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 475 GAATTT [GA]^AATT[CT]
3379 AAATTT
Aor51HI 1287 AGCGCT AGC^GCT
1679 AGCGCT
ApoI 475 GAATTT [GA]^AATT[CT]
3379 AAATTT
Asp700I 1041 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4587 GAAGGGCTTC
BcgI 2058 CGACACCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3157 CGAATTGGGTGC
BsaAI 4379 TACGTA [CT]AC^GT[GA]
4415 CACGTG
BsmBI 510 CGTCTC CGTCTC(1/5)
1417 GAGACG
BsrDI 1376 GCAATG GCAATG(2/0)
4041 GCAATG
BssSI 1363 CACGAG CACGAG(-5/-1)
3188 CACGAG
BstEII 3466 GGTCACC G^GT[ACGT]ACC
3918 GGTGACC
BstPI 3466 GGTCACC G^GT[ACGT]ACC
3918 GGTGACC
Eco47III 1287 AGCGCT AGC^GCT
1679 AGCGCT
EcoO65I 3466 GGTCACC G^GT[ACGT]ACC
3918 GGTGACC
MslI 198 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1260 CACATCCATG
NheI 2081 GCTAGC G^CTAGC
2956 GCTAGC
NspI 929 ACATGC [GA]CATG^[CT]
1340 ACATGC
PshAI 1932 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4355 GACTTGGGTC
XmnI 1041 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4587 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
AspHI AspI AvaI AvaII AvrII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XhoII