##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AviII BamHI BbeI
BcgI BclI BfrI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI EagI
Eam1105I EclXI Eco52I EcoO65I EcoT22I
FbaI FseI FspI KasI KpnI
KspI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI SacII SalI SbfI
SfoI SfuI SnaBI Sse8387I SspBI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 1841 GACGTC GACGT^C
AflIII 2585 ACATGT A^C[GA][CT]GT
Aor51HI 852 AGCGCT AGC^GCT
ApaI 3404 GGGCCC GGGCC^C
BciVI 2467 GGATAC GTATCC(6/5)
BsaBI 353 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 4653 GAGACG CGTCTC(1/5)
BspLU11I 2585 ACATGT A^CATGT
BsrBI 2446 CCGCTC CCGCTC(-3/-3)
BssHII 3204 GCGCGC G^CGCGC
CpoI 123 CGGACCG CG^G[AT]CCG
DraI 4527 TTTAAA TTT^AAA
Eco47III 852 AGCGCT AGC^GCT
EcoRV 1317 GATATC GAT^ATC
HaeII 852 AGCGCT [GA]GCGC^[CT]
HincII 3864 GTTAAC GT[CT]^[GA]AC
HindII 3864 GTTAAC GT[CT]^[GA]AC
HpaI 3864 GTTAAC GTT^AAC
MamI 353 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 743 CTCGAG C^TCGAG
RsrII 123 CGGACCG CG^G[AT]CCG
SacI 1935 GAGCTC GAGCT^C
SapI 818 GAAGAGC GCTCTTC(1/4)
SexAI 551 ACCTGGT A^CC[AT]GGT
SfiI 148 GGCCGCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1090 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 3002 ACTAGT A^CTAGT
SphI 1816 GCATGC GCATG^C
SspI 4666 AATATT AAT^ATT
XhoI 743 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Alw44I 1855 GTGCAC G^TGCAC
2367 GTGCAC
AlwI 1334 GGATC GGATC(4/5)
4783 GGATC
ApaLI 1855 GTGCAC G^TGCAC
2367 GTGCAC
Asp700I 1973 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2551 GAAAGCATTC
AspI 271 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4584 GACAGGGTC
BbvCI 1641 CCTCAGC CCTCAGC(-5/-2)
3361 GCTGAGG
BsrDI 2894 CATTGC GCAATG(2/0)
3884 GCAATG
BsrFI 1091 GCCGGT [GA]^CCGG[CT]
2304 ACCGGC
Cfr10I 1091 GCCGGT [GA]^CCGG[CT]
2304 ACCGGC
EcoNI 772 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3395 CCTTGTCCAGG
HindIII 1960 AAGCTT A^AGCTT
3764 AAGCTT
PflFI 271 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4584 GACAGGGTC
PflMI 2133 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2905 CCACCCTGTGG
ScaI 2252 AGTACT AGT^ACT
4056 AGTACT
Tth111I 271 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4584 GACAGGGTC
Van91I 2133 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2905 CCACCCTGTGG
XmnI 1973 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2551 GAAAGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwNI AocI ApoI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbrPI BbsI BbvI BcnI
BfaI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI EarI
Eco81I EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI