##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AcyI AflII AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI BamHI BbeI
BbrPI BfrI BglI BsaHI BsiEI
BsiWI BspDI BspLU11I BsrBI BssHII
BstBI ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
FauI FseI HaeII Hin1I KasI
KpnI KspI MluI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 5197 GTATAC GT^[AC][GT]AC
AccII 184 CGCG CG^CG
AccIII 653 TCCGGA T^CCGGA
AclI 724 AACGTT AA^CGTT
AgeI 1870 ACCGGT A^CCGGT
AvaI 5 CTCGGG C^[CT]CG[GA]G
AviII 2785 TGCGCA TGC^GCA
AvrII 1242 CCTAGG C^CTAGG
BcgI 1313 CGACTCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1079 CCCGG CC^[GC]GG
BglII 4617 AGATCT A^GATCT
BlnI 1242 CCTAGG C^CTAGG
BlpI 10 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 10 GCTCAGC GC^T[ACGT]AGC
BsaAI 2408 CACGTA [CT]AC^GT[GA]
BsaBI 4349 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 653 TCCGGA T^CCGGA
BsoBI 5 CTCGGG C^[CT]CG[GA]G
BspEI 653 TCCGGA T^CCGGA
BspHI 3536 TCATGA T^CATGA
BsrDI 3279 GCAATG GCAATG(2/0)
BssSI 208 CTCGTG CACGAG(-5/-1)
Bst1107I 5197 GTATAC GTA^TAC
BstEII 4766 GGTGACC G^GT[ACGT]ACC
BstPI 4766 GGTGACC G^GT[ACGT]ACC
BstUI 184 CGCG CG^CG
BstZ17I 5197 GTATAC GTA^TAC
CelII 10 GCTCAGC GC^T[ACGT]AGC
DraIII 4391 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 4766 GGTGACC G^GT[ACGT]ACC
FspI 2785 TGCGCA TGC^GCA
HindIII 2258 AAGCTT A^AGCTT
HpaI 2294 GTTAAC GTT^AAC
MamI 4349 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 653 TCCGGA T^CCGGA
MvnI 184 CGCG CG^CG
NciI 1079 CCCGG CC^[GC]GG
PflMI 75 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1870 ACCGGT A^CCGGT
Psp1406I 724 AACGTT AA^CGTT
RcaI 3536 TCATGA T^CATGA
SacI 757 GAGCTC GAGCT^C
SphI 1115 GCATGC GCATG^C
SspI 824 AATATT AAT^ATT
Van91I 75 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AflIII 2407 ACACGT A^C[GA][CT]GT
5302 ACGTGT
AocI 2499 CCTCAGG CC^T[ACGT]AGG
4752 CCTCAGG
BalI 446 TGGCCA TGG^CCA
980 TGGCCA
BanI 1779 GGCACC G^G[CT][GA]CC
4748 GGCACC
BbvCI 1634 CCTCAGC CCTCAGC(-5/-2)
1846 GCTGAGG
BciVI 3183 GTATCC GTATCC(6/5)
3977 GTATCC
BclI 1055 TGATCA T^GATCA
5217 TGATCA
BsaI 5064 GAGACC GGTCTC(1/5)
2962 GGTCTC
Bsp1407I 603 TGTACA T^GTACA
4548 TGTACA
BspMI 1909 ACCTGC ACCTGC(4/8)
5654 GCAGGT
BsrFI 1870 ACCGGT [GA]^CCGG[CT]
4745 ACCGGC
BsrGI 603 TGTACA T^GTACA
4548 TGTACA
BstAPI 1399 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2841 GCATGGGGTGC
Bsu36I 2499 CCTCAGG CC^T[ACGT]AGG
4752 CCTCAGG
CfoI 183 GCGC GCG^C
2786 GCGC
Cfr10I 1870 ACCGGT [GA]^CCGG[CT]
4745 ACCGGC
DrdI 892 GACACAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4769 GACCAGGTTGTC
DsaI 1490 CCATGG C^C[GA][CT]GG
2865 CCATGG
Eco81I 2499 CCTCAGG CC^T[ACGT]AGG
4752 CCTCAGG
EcoNI 2615 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2680 CCTCCTGGAGG
EcoT22I 283 ATGCAT ATGCA^T
2725 ATGCAT
FbaI 1055 TGATCA T^GATCA
5217 TGATCA
HhaI 183 GCGC GCG^C
2786 GCGC
HinP1I 183 GCGC G^CGC
2786 GCGC
MfeI 5113 CAATTG C^AATTG
5266 CAATTG
MluNI 446 TGGCCA TGG^CCA
980 TGGCCA
MscI 446 TGGCCA TGG^CCA
980 TGGCCA
MunI 5113 CAATTG C^AATTG
5266 CAATTG
NcoI 1490 CCATGG C^CATGG
2865 CCATGG
NsiI 283 ATGCAT ATGCA^T
2725 ATGCAT
PpuMI 3342 AGGTCCC [GA]G^G[AT]CC[CT]
4808 AGGACCT
PshAI 4373 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5425 GACAAGTGTC
SapI 544 GAAGAGC GCTCTTC(1/4)
2374 GAAGAGC
SexAI 3767 ACCTGGT A^CC[AT]GGT
4770 ACCAGGT
SpeI 2170 ACTAGT A^CTAGT
4625 ACTAGT
SspBI 603 TGTACA T^GTACA
4548 TGTACA
StuI 2823 AGGCCT AGG^CCT
3519 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XcmI XhoII XmnI