##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccIII AclI AgeI Aor51HI ApaI
AscI AseI AsnI AvrII BbeI
BbrPI BbvCI BglII BlnI BseAI
BsiWI BsmI Bsp1407I BspEI BsrBI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstXI Eco47III EcoNI
EcoO65I FseI HincII HindII HpaI
KasI KspI MluI MroI NarI
NotI NruI NspV PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
Sse8387I SspBI SwaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1571 GACGTC GACGT^C
Acc65I 3723 GGTACC G^GTACC
Asp718I 3723 GGTACC G^GTACC
AviII 144 TGCGCA TGC^GCA
BamHI 698 GGATCC G^GATCC
BanI 3723 GGTACC G^G[CT][GA]CC
BcgI 1761 GCAGACAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2775 TGATCA T^GATCA
BglI 1326 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1614 GCTCAGC GC^T[ACGT]AGC
BpmI 1013 CTGGAG CTGGAG(16/14)
Bpu1102I 1614 GCTCAGC GC^T[ACGT]AGC
BsaAI 4335 TACGTA [CT]AC^GT[GA]
BsaWI 213 TCCGGT [AT]^CCGG[AT]
BseRI 4122 GAGGAG GAGGAG(10/8)
BsmBI 345 GAGACG CGTCTC(1/5)
BsmFI 3425 GTCCC GGGAC(10/14)
BspDI 1714 ATCGAT AT^CGAT
Bst1107I 4561 GTATAC GTA^TAC
BstZ17I 4561 GTATAC GTA^TAC
CelII 1614 GCTCAGC GC^T[ACGT]AGC
ClaI 1714 ATCGAT AT^CGAT
CpoI 255 CGGTCCG CG^G[AT]CCG
DraII 526 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 3359 GACTCTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 526 GGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 2775 TGATCA T^GATCA
FspI 144 TGCGCA TGC^GCA
HgaI 134 GCGTC GACGC(5/10)
KpnI 3723 GGTACC GGTAC^C
MfeI 4362 CAATTG C^AATTG
MunI 4362 CAATTG C^AATTG
NheI 3787 GCTAGC G^CTAGC
PacI 3439 TTAATTAA TTAAT^TAA
PaeR7I 353 CTCGAG C^TCGAG
PpuMI 526 GGGTCCT [GA]G^G[AT]CC[CT]
RsrII 255 CGGTCCG CG^G[AT]CCG
ScaI 3177 AGTACT AGT^ACT
SmaI 225 CCCGGG CCC^GGG
SnaBI 4335 TACGTA TAC^GTA
SpeI 1382 ACTAGT A^CTAGT
SphI 2047 GCATGC GCATG^C
StuI 2913 AGGCCT AGG^CCT
XbaI 4463 TCTAGA T^CTAGA
XhoI 353 CTCGAG C^TCGAG
XmaCI 225 CCCGGG C^CCGGG
XmaI 225 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 133 GGCGTC G[GA]^CG[CT]C
1571 GACGTC
AflIII 4190 ACATGT A^C[GA][CT]GT
4611 ACATGT
AhdI 85 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
408 GACTCCTTGTC
Alw44I 187 GTGCAC G^TGCAC
4419 GTGCAC
AlwNI 463 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2758 CAGTAACTG
AocI 530 CCTGAGG CC^T[ACGT]AGG
840 CCTGAGG
ApaLI 187 GTGCAC G^TGCAC
4419 GTGCAC
AspEI 85 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
408 GACTCCTTGTC
AvaI 225 CCCGGG C^[CT]CG[GA]G
353 CTCGAG
BalI 601 TGGCCA TGG^CCA
1733 TGGCCA
BsaHI 133 GGCGTC G[GA]^CG[CT]C
1571 GACGTC
BsaI 4268 GAGACC GGTCTC(1/5)
216 GGTCTC
BsiEI 99 CGGCCG CG[GA][CT]^CG
393 CGGCCG
BsoBI 225 CCCGGG C^[CT]CG[GA]G
353 CTCGAG
BspHI 2700 TCATGA T^CATGA
3015 TCATGA
BspLU11I 4190 ACATGT A^CATGT
4611 ACATGT
BsrDI 1587 CATTGC GCAATG(2/0)
2296 CATTGC
BstYI 698 GGATCC [GA]^GATC[CT]
858 AGATCC
Bsu36I 530 CCTGAGG CC^T[ACGT]AGG
840 CCTGAGG
EagI 99 CGGCCG C^GGCCG
393 CGGCCG
Eam1105I 85 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
408 GACTCCTTGTC
EarI 4482 CTCTTC CTCTTC(1/4)
3913 GAAGAG
EclXI 99 CGGCCG C^GGCCG
393 CGGCCG
Eco52I 99 CGGCCG C^GGCCG
393 CGGCCG
Eco81I 530 CCTGAGG CC^T[ACGT]AGG
840 CCTGAGG
EcoRV 1395 GATATC GAT^ATC
2722 GATATC
EcoT22I 886 ATGCAT ATGCA^T
3839 ATGCAT
HaeII 233 GGCGCT [GA]GCGC^[CT]
636 GGCGCT
Hin1I 133 GGCGTC G[GA]^CG[CT]C
1571 GACGTC
Ksp632I 4482 CTCTTC CTCTTC(1/4)
3913 GAAGAG
MflI 698 GGATCC [GA]^GATC[CT]
858 AGATCC
MluNI 601 TGGCCA TGG^CCA
1733 TGGCCA
MscI 601 TGGCCA TGG^CCA
1733 TGGCCA
NcoI 1201 CCATGG C^CATGG
1736 CCATGG
NdeI 2458 CATATG CA^TATG
3392 CATATG
NsiI 886 ATGCAT ATGCA^T
3839 ATGCAT
PflMI 627 CCATTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1196 CCAGCCCATGG
PstI 4048 CTGCAG CTGCA^G
4379 CTGCAG
PvuII 1880 CAGCTG CAG^CTG
1980 CAGCTG
RcaI 2700 TCATGA T^CATGA
3015 TCATGA
SacI 350 GAGCTC GAGCT^C
2061 GAGCTC
Van91I 627 CCATTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1196 CCAGCCCATGG
XhoII 698 GGATCC [GA]^GATC[CT]
858 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AluI AlwI ApoI Asp700I
AspHI AspI AvaII BanII BbsI
BbvI BciVI BcnI BfaI BfrI
BmyI BpuAI BsaBI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraIII
DsaI EaeI EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MamI MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XmnI