##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
Aor51HI ApaI AscI Asp718I AvaI
AviII BamHI BanII BbeI BbrPI
BglI BsaAI BsaBI BsiEI BsiWI
BsmBI BsoBI BsrBI BsrFI BssHII
BstUI BstXI CfoI Cfr10I CpoI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I EcoNI FseI FspI
HaeII HhaI HinP1I HpaI KasI
KpnI KspI MamI MluI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccIII 3212 TCCGGA T^CCGGA
AcyI 3549 GACGCC G[GA]^CG[CT]C
AflII 334 CTTAAG C^TTAAG
AhdI 2898 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 29 GTGCAC G^TGCAC
AocI 848 CCTCAGG CC^T[ACGT]AGG
ApaLI 29 GTGCAC G^TGCAC
AspEI 2898 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 163 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2593 CCTAGG C^CTAGG
BbvCI 1336 CCTCAGC CCTCAGC(-5/-2)
BcgI 1262 GCAATCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3372 GGATAC GTATCC(6/5)
BclI 3129 TGATCA T^GATCA
BcnI 3834 CCGGG CC^[GC]GG
BfrI 334 CTTAAG C^TTAAG
BlnI 2593 CCTAGG C^CTAGG
BlpI 109 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 109 GCTCAGC GC^T[ACGT]AGC
BsaHI 3549 GACGCC G[GA]^CG[CT]C
BsaI 5719 GAGACC GGTCTC(1/5)
BsaWI 3212 TCCGGA [AT]^CCGG[AT]
BseAI 3212 TCCGGA T^CCGGA
BspDI 46 ATCGAT AT^CGAT
BspEI 3212 TCCGGA T^CCGGA
BspMI 4352 ACCTGC ACCTGC(4/8)
BssSI 659 CTCGTG CACGAG(-5/-1)
BstBI 1616 TTCGAA TT^CGAA
BstEII 6425 GGTTACC G^GT[ACGT]ACC
BstPI 6425 GGTTACC G^GT[ACGT]ACC
Bsu36I 848 CCTCAGG CC^T[ACGT]AGG
CelII 109 GCTCAGC GC^T[ACGT]AGC
ClaI 46 ATCGAT AT^CGAT
Eam1105I 2898 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1099 GAAGAG CTCTTC(1/4)
Eco81I 848 CCTCAGG CC^T[ACGT]AGG
EcoO65I 6425 GGTTACC G^GT[ACGT]ACC
EcoRI 1493 GAATTC G^AATTC
EcoRV 2052 GATATC GAT^ATC
EcoT14I 2593 CCTAGG C^C[AT][AT]GG
FbaI 3129 TGATCA T^GATCA
Hin1I 3549 GACGCC G[GA]^CG[CT]C
Ksp632I 1099 GAAGAG CTCTTC(1/4)
MfeI 2926 CAATTG C^AATTG
MroI 3212 TCCGGA T^CCGGA
MunI 2926 CAATTG C^AATTG
NciI 3834 CCGGG CC^[GC]GG
NspV 1616 TTCGAA TT^CGAA
PacI 4195 TTAATTAA TTAAT^TAA
PflFI 163 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3474 GGGTCCT [GA]G^G[AT]CC[CT]
ScaI 1657 AGTACT AGT^ACT
SfuI 1616 TTCGAA TT^CGAA
StyI 2593 CCTAGG C^C[AT][AT]GG
Tth111I 163 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6619 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwI 983 GATCC GGATC(4/5)
2230 GATCC
AseI 4194 ATTAAT AT^TAAT
6200 ATTAAT
AsnI 4194 ATTAAT AT^TAAT
6200 ATTAAT
BalI 38 TGGCCA TGG^CCA
4866 TGGCCA
BanI 1450 GGTGCC G^G[CT][GA]CC
6735 GGCACC
BglII 141 AGATCT A^GATCT
3856 AGATCT
BspLU11I 5075 ACATGT A^CATGT
5674 ACATGT
BstAPI 324 GCAAACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
397 GCATTAGCTGC
DraII 3474 GGGTCCT [GA]G^G[ACGT]CC[CT]
3912 AGGCCCT
EaeI 38 TGGCCA [CT]^GGCC[GA]
4866 TGGCCA
EcoO109I 3474 GGGTCCT [GA]G^G[ACGT]CC[CT]
3912 AGGCCCT
EcoT22I 1586 ATGCAT ATGCA^T
3629 ATGCAT
HapII 3213 CCGG C^CGG
3834 CCGG
HgaI 163 GACGC GACGC(5/10)
3549 GACGC
HincII 2859 GTCAAC GT[CT]^[GA]AC
6334 GTCAAC
HindII 2859 GTCAAC GT[CT]^[GA]AC
6334 GTCAAC
HpaII 3213 CCGG C^CGG
3834 CCGG
MluNI 38 TGGCCA TGG^CCA
4866 TGGCCA
MscI 38 TGGCCA TGG^CCA
4866 TGGCCA
MspA1I 499 CAGCTG C[AC]G^C[GT]G
1891 CAGCTG
MspI 3213 CCGG C^CGG
3834 CCGG
NdeI 5451 CATATG CA^TATG
5773 CATATG
NsiI 1586 ATGCAT ATGCA^T
3629 ATGCAT
PflMI 1087 CCAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1890 CCAGCTGTTGG
PshBI 4194 ATTAAT AT^TAAT
6200 ATTAAT
PstI 496 CTGCAG CTGCA^G
6237 CTGCAG
PvuII 499 CAGCTG CAG^CTG
1891 CAGCTG
SpeI 4627 ACTAGT A^CTAGT
5875 ACTAGT
StuI 2478 AGGCCT AGG^CCT
5527 AGGCCT
Van91I 1087 CCAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1890 CCAGCTGTTGG
XcmI 1347 CCAAATTGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2798 CCAAAGGGACTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwNI ApoI Asp700I AspHI
AvaII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspHI BsrDI
BsrGI BsrI BssKI Bst1107I BstF5I
BstNI BstYI BstZ17I Cac8I Cfr13I
DdeI DpnII DraI EcoRII FauI
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI