##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AclI AhdI Aor51HI AscI AseI
AsnI AspEI AvrII BbeI BbrPI
BbvCI BcgI BglI BlnI BsaAI
BsiEI BsiWI BspDI BsrBI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoT22I FseI HaeII HpaI
KasI KspI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspI SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 3504 GGTACC G^GTACC
AccI 4251 GTAGAC GT^[AC][GT]AC
AccII 592 CGCG CG^CG
AccIII 139 TCCGGA T^CCGGA
AflII 4156 CTTAAG C^TTAAG
AflIII 3616 ACATGT A^C[GA][CT]GT
AgeI 345 ACCGGT A^CCGGT
Alw44I 3835 GTGCAC G^TGCAC
ApaI 2338 GGGCCC GGGCC^C
ApaLI 3835 GTGCAC G^TGCAC
Asp718I 3504 GGTACC G^GTACC
AviII 787 TGCGCA TGC^GCA
BamHI 2350 GGATCC G^GATCC
BciVI 4452 GTATCC GTATCC(6/5)
BclI 42 TGATCA T^GATCA
BfrI 4156 CTTAAG C^TTAAG
BsaBI 2351 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 139 TCCGGA T^CCGGA
BsgI 4516 CTGCAC GTGCAG(16/14)
BsmBI 159 GAGACG CGTCTC(1/5)
Bsp1407I 639 TGTACA T^GTACA
BspEI 139 TCCGGA T^CCGGA
BspLU11I 3616 ACATGT A^CATGT
BsrDI 4525 CATTGC GCAATG(2/0)
BsrGI 639 TGTACA T^GTACA
BstEII 664 GGTCACC G^GT[ACGT]ACC
BstPI 664 GGTCACC G^GT[ACGT]ACC
BstUI 592 CGCG CG^CG
DraI 3952 TTTAAA TTT^AAA
DsaI 2316 CCATGG C^C[GA][CT]GG
EcoO65I 664 GGTCACC G^GT[ACGT]ACC
FauI 487 CCCGC CCCGC(4/6)
FbaI 42 TGATCA T^GATCA
FspI 787 TGCGCA TGC^GCA
HgaI 161 GACGC GACGC(5/10)
KpnI 3504 GGTACC GGTAC^C
MamI 2351 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 139 TCCGGA T^CCGGA
MvnI 592 CGCG CG^CG
NaeI 1354 GCCGGC GCC^GGC
NcoI 2316 CCATGG C^CATGG
NgoMIV 1354 GCCGGC G^CCGGC
PaeR7I 3279 CTCGAG C^TCGAG
PinAI 345 ACCGGT A^CCGGT
SbfI 505 CCTGCAGG CCTGCA^GG
ScaI 3179 AGTACT AGT^ACT
Sse8387I 505 CCTGCAGG CCTGCA^GG
SspBI 639 TGTACA T^GTACA
XhoI 3279 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 1664 GACGTC GACGT^C
2460 GACGTC
AcyI 1664 GACGTC G[GA]^CG[CT]C
2460 GACGTC
AvaI 323 CCCGAG C^[CT]CG[GA]G
3279 CTCGAG
BcnI 661 CCGGG CC^[GC]GG
3450 CCCGG
BsaHI 1664 GACGTC G[GA]^CG[CT]C
2460 GACGTC
BsmI 2153 GAATGC GAATGC(1/-1)
1379 GCATTC
BsoBI 323 CCCGAG C^[CT]CG[GA]G
3279 CTCGAG
BsrFI 345 ACCGGT [GA]^CCGG[CT]
1354 GCCGGC
BstAPI 448 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4712 GCACTCAGTGC
CfoI 788 GCGC GCG^C
1545 GCGC
Cfr10I 345 ACCGGT [GA]^CCGG[CT]
1354 GCCGGC
DraIII 1964 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4713 CACTCAGTG
EcoRI 1206 GAATTC G^AATTC
2768 GAATTC
HhaI 788 GCGC GCG^C
1545 GCGC
Hin1I 1664 GACGTC G[GA]^CG[CT]C
2460 GACGTC
HinP1I 788 GCGC G^CGC
1545 GCGC
MaeII 1665 ACGT A^CGT
2461 ACGT
NciI 661 CCGGG CC^[GC]GG
3450 CCCGG
SexAI 1045 ACCAGGT A^CC[AT]GGT
2036 ACCTGGT
XcmI 236 CCACCATCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1064 CCAGGTTGGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AspI AvaII BalI BanI BanII
BbsI BbvI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
Cfr13I DdeI DpnII DraII DrdI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoRV EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII XmnI