##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbeI
BciVI BclI BlnI BsaWI BseAI
BsiEI BsmBI BspDI BspEI BssHII
Bst1107I BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I FbaI
FspI KasI KpnI KspI MluI
MroI NarI NotI NruI PacI
PaeR7I PflMI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfoI SgrAI SnaBI SpeI
Sse8387I SwaI Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 410 GACGTC GACGT^C
AccI 5056 GTAGAC GT^[AC][GT]AC
AccII 2492 CGCG CG^CG
Alw44I 2471 GTGCAC G^TGCAC
ApaLI 2471 GTGCAC G^TGCAC
BamHI 833 GGATCC G^GATCC
BbrPI 5738 CACGTG CAC^GTG
BlpI 937 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 937 GCTGAGC GC^T[ACGT]AGC
BsaAI 5738 CACGTG [CT]AC^GT[GA]
BsaBI 801 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 6648 CGTACG C^GTACG
BspHI 4823 TCATGA T^CATGA
BsrFI 6499 GCCGGC [GA]^CCGG[CT]
BstBI 974 TTCGAA TT^CGAA
BstEII 214 GGTCACC G^GT[ACGT]ACC
BstPI 214 GGTCACC G^GT[ACGT]ACC
BstUI 2492 CGCG CG^CG
CelII 937 GCTGAGC GC^T[ACGT]AGC
Cfr10I 6499 GCCGGC [GA]^CCGG[CT]
DraIII 5781 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4355 GACCCTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 214 GGTCACC G^GT[ACGT]ACC
EcoRV 5898 GATATC GAT^ATC
EcoT22I 1213 ATGCAT ATGCA^T
FseI 6498 GGCCGGCC GGCCGG^CC
HaeII 5762 AGCGCC [GA]GCGC^[CT]
HpaI 4158 GTTAAC GTT^AAC
MamI 801 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2492 CGCG CG^CG
NaeI 6499 GCCGGC GCC^GGC
NgoMIV 6499 GCCGGC G^CCGGC
NsiI 1213 ATGCAT ATGCA^T
NspV 974 TTCGAA TT^CGAA
PmaCI 5738 CACGTG CAC^GTG
PmlI 5738 CACGTG CAC^GTG
PshAI 3411 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4823 TCATGA T^CATGA
SacI 4469 GAGCTC GAGCT^C
ScaI 1366 AGTACT AGT^ACT
SfiI 6580 GGCCAGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 974 TTCGAA TT^CGAA
SmaI 3319 CCCGGG CCC^GGG
SphI 2079 GCATGC GCATG^C
SspI 4644 AATATT AAT^ATT
StuI 4058 AGGCCT AGG^CCT
XmaCI 3319 CCCGGG C^CCGGG
XmaI 3319 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 410 GACGTC G[GA]^CG[CT]C
2866 GACGCC
AocI 2099 CCTCAGG CC^T[ACGT]AGG
3486 CCTGAGG
AspI 4303 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5302 GACATGGTC
AvaI 1034 CTCGGG C^[CT]CG[GA]G
3319 CCCGGG
BanI 3534 GGTGCC G^G[CT][GA]CC
3552 GGCACC
BbvCI 4551 CCTCAGC CCTCAGC(-5/-2)
6543 GCTGAGG
BcgI 4955 GCATGTACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6678 GCAGGAGAATCG
BglII 1995 AGATCT A^GATCT
6256 AGATCT
BsaHI 410 GACGTC G[GA]^CG[CT]C
2866 GACGCC
BsmI 1230 GAATGC GAATGC(1/-1)
5812 GCATTC
BsoBI 1034 CTCGGG C^[CT]CG[GA]G
3319 CCCGGG
BsrDI 4564 CATTGC GCAATG(2/0)
5518 CATTGC
BssSI 4558 CTCGTG CACGAG(-5/-1)
4963 CTCGTG
BstAPI 5760 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6704 GCAGAGGCTGC
Bsu36I 2099 CCTCAGG CC^T[ACGT]AGG
3486 CCTGAGG
CfoI 2491 GCGC GCG^C
5763 GCGC
DraI 306 TTTAAA TTT^AAA
5225 TTTAAA
Eco81I 2099 CCTCAGG CC^T[ACGT]AGG
3486 CCTGAGG
EcoRI 4819 GAATTC G^AATTC
4969 GAATTC
HgaI 2866 GACGC GACGC(5/10)
2976 GACGC
HhaI 2491 GCGC GCG^C
5763 GCGC
Hin1I 410 GACGTC G[GA]^CG[CT]C
2866 GACGCC
HinP1I 2491 GCGC G^CGC
5763 GCGC
HindIII 734 AAGCTT A^AGCTT
1054 AAGCTT
NcoI 2690 CCATGG C^CATGG
3781 CCATGG
NdeI 4348 CATATG CA^TATG
6141 CATATG
PflFI 4303 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5302 GACATGGTC
PpuMI 5 GGGACCT [GA]G^G[AT]CC[CT]
842 GGGACCT
SapI 2569 GAAGAGC GCTCTTC(1/4)
2839 GCTCTTC
SexAI 2272 ACCAGGT A^CC[AT]GGT
3724 ACCTGGT
Tth111I 4303 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5302 GACATGGTC
XbaI 4799 TCTAGA T^CTAGA
6049 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BanII BbsI
BbvI BcnI BfaI BfrI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I
BspMI BsrBI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MunI MvaI MwoI
NciI NdeII NheI NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI