##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
BamHI BciVI BfrI BsiWI BspDI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV HpaI MluI NheI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI SwaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 2905 TCCGGA T^CCGGA
AcyI 374 GGCGCC G[GA]^CG[CT]C
AflIII 5204 ACATGT A^C[GA][CT]GT
AhdI 3487 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3806 CCTGAGG CC^T[ACGT]AGG
AspEI 3487 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 387 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 205 TGCGCA TGC^GCA
BbeI 374 GGCGCC GGCGC^C
BbrPI 5816 CACGTG CAC^GTG
BsaAI 5816 CACGTG [CT]AC^GT[GA]
BsaBI 4802 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 374 GGCGCC G[GA]^CG[CT]C
BseAI 2905 TCCGGA T^CCGGA
BsiEI 1440 CGGTCG CG[GA][CT]^CG
BspEI 2905 TCCGGA T^CCGGA
BspLU11I 5204 ACATGT A^CATGT
Bsu36I 3806 CCTGAGG CC^T[ACGT]AGG
DraIII 3271 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3487 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3806 CCTGAGG CC^T[ACGT]AGG
FspI 205 TGCGCA TGC^GCA
Hin1I 374 GGCGCC G[GA]^CG[CT]C
KasI 374 GGCGCC G^GCGCC
KspI 166 CCGCGG CCGC^GG
MamI 4802 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2905 TCCGGA T^CCGGA
NarI 374 GGCGCC GG^CGCC
NcoI 2236 CCATGG C^CATGG
PaeR7I 211 CTCGAG C^TCGAG
PflFI 387 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3125 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 5816 CACGTG CAC^GTG
PmlI 5816 CACGTG CAC^GTG
PshAI 4495 GACTACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 166 CCGCGG CCGC^GG
SalI 3463 GTCGAC G^TCGAC
SfoI 374 GGCGCC GGC^GCC
SmaI 3421 CCCGGG CCC^GGG
Tth111I 387 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3125 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3029 TCTAGA T^CTAGA
XhoI 211 CTCGAG C^TCGAG
XmaCI 3421 CCCGGG C^CCGGG
XmaI 3421 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 2842 GGTACC G^GTACC
5035 GGTACC
Asp718I 2842 GGTACC G^GTACC
5035 GGTACC
AvrII 5108 CCTAGG C^CTAGG
5362 CCTAGG
BcgI 4853 CGAGGGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2813 GCACAAGTGTCG
BclI 622 TGATCA T^GATCA
5006 TGATCA
BglI 216 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6536 GCCCTGCAGGC
BlnI 5108 CCTAGG C^CTAGG
5362 CCTAGG
BlpI 2200 GCTCAGC GC^T[ACGT]AGC
2637 GCTGAGC
Bpu1102I 2200 GCTCAGC GC^T[ACGT]AGC
2637 GCTGAGC
BsmBI 5808 CGTCTC CGTCTC(1/5)
5845 GAGACG
Bsp1407I 4699 TGTACA T^GTACA
6287 TGTACA
BsrDI 419 CATTGC GCAATG(2/0)
4097 CATTGC
BsrGI 4699 TGTACA T^GTACA
6287 TGTACA
CelII 2200 GCTCAGC GC^T[ACGT]AGC
2637 GCTGAGC
DraI 6165 TTTAAA TTT^AAA
6579 TTTAAA
EcoNI 395 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5225 CCTGACTCAGG
EcoT22I 1664 ATGCAT ATGCA^T
2789 ATGCAT
FbaI 622 TGATCA T^GATCA
5006 TGATCA
FseI 67 GGCCGGCC GGCCGG^CC
2682 GGCCGGCC
HaeII 17 GGCGCT [GA]GCGC^[CT]
374 GGCGCC
HgaI 87 GACGC GACGC(5/10)
5807 GCGTC
HincII 3463 GTCGAC GT[CT]^[GA]AC
5533 GTTGAC
HindII 3463 GTCGAC GT[CT]^[GA]AC
5533 GTTGAC
HindIII 2486 AAGCTT A^AGCTT
3894 AAGCTT
KpnI 2842 GGTACC GGTAC^C
5035 GGTACC
MfeI 4280 CAATTG C^AATTG
4846 CAATTG
MunI 4280 CAATTG C^AATTG
4846 CAATTG
NdeI 4484 CATATG CA^TATG
6234 CATATG
NsiI 1664 ATGCAT ATGCA^T
2789 ATGCAT
SacI 1772 GAGCTC GAGCT^C
4359 GAGCTC
SbfI 2774 CCTGCAGG CCTGCA^GG
6538 CCTGCAGG
SexAI 4578 ACCAGGT A^CC[AT]GGT
5146 ACCAGGT
Sse8387I 2774 CCTGCAGG CCTGCA^GG
6538 CCTGCAGG
SspBI 4699 TGTACA T^GTACA
6287 TGTACA
SspI 3396 AATATT AAT^ATT
6415 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI