##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AcyI AflIII AgeI
AhdI Alw44I ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
BbeI BbvCI BcgI BglI BsaHI
BsaI BsiWI BsmBI BsoBI Bsp1407I
BspLU11I BsrBI BsrFI BsrGI BssHII
BstBI BstEII BstPI BstXI Cfr10I
CpoI DrdI Eam1105I EcoNI EcoO65I
EcoRI FseI FspI HgaI Hin1I
KasI KpnI KspI MluI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccI 5143 GTATAC GT^[AC][GT]AC
AclI 4969 AACGTT AA^CGTT
BamHI 5431 GGATCC G^GATCC
BbrPI 750 CACGTG CAC^GTG
BciVI 1177 GTATCC GTATCC(6/5)
BglII 2970 AGATCT A^GATCT
BlpI 3441 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3441 GCTGAGC GC^T[ACGT]AGC
BsaAI 750 CACGTG [CT]AC^GT[GA]
BsiEI 77 CGGCCG CG[GA][CT]^CG
BspDI 976 ATCGAT AT^CGAT
BssSI 5346 CTCGTG CACGAG(-5/-1)
Bst1107I 5143 GTATAC GTA^TAC
BstZ17I 5143 GTATAC GTA^TAC
CelII 3441 GCTGAGC GC^T[ACGT]AGC
ClaI 976 ATCGAT AT^CGAT
DraIII 750 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 77 CGGCCG C^GGCCG
EclXI 77 CGGCCG C^GGCCG
Eco52I 77 CGGCCG C^GGCCG
EcoRV 870 GATATC GAT^ATC
FauI 2883 CCCGC CCCGC(4/6)
MfeI 2364 CAATTG C^AATTG
MunI 2364 CAATTG C^AATTG
NcoI 2596 CCATGG C^CATGG
PmaCI 750 CACGTG CAC^GTG
PmlI 750 CACGTG CAC^GTG
Psp1406I 4969 AACGTT AA^CGTT
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 107 CGCG CG^CG
1536 CGCG
AccIII 2109 TCCGGA T^CCGGA
3076 TCCGGA
AflII 2717 CTTAAG C^TTAAG
5067 CTTAAG
AocI 4153 CCTTAGG CC^T[ACGT]AGG
4466 CCTCAGG
Aor51HI 1468 AGCGCT AGC^GCT
4424 AGCGCT
AvrII 1094 CCTAGG C^CTAGG
2671 CCTAGG
BanI 263 GGTGCC G^G[CT][GA]CC
2810 GGCACC
BbsI 4286 GAAGAC GAAGAC(2/6)
2395 GTCTTC
BfrI 2717 CTTAAG C^TTAAG
5067 CTTAAG
BlnI 1094 CCTAGG C^CTAGG
2671 CCTAGG
BpuAI 4286 GAAGAC GAAGAC(2/6)
2395 GTCTTC
BsaBI 1551 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4457 GATCCTTATC
BsaWI 2109 TCCGGA [AT]^CCGG[AT]
3076 TCCGGA
BseAI 2109 TCCGGA T^CCGGA
3076 TCCGGA
BsgI 1203 GTGCAG GTGCAG(16/14)
1255 GTGCAG
BsmFI 161 GGGAC GGGAC(10/14)
5487 GTCCC
BspEI 2109 TCCGGA T^CCGGA
3076 TCCGGA
BspHI 1477 TCATGA T^CATGA
5211 TCATGA
BsrDI 1151 CATTGC GCAATG(2/0)
5524 CATTGC
BstUI 107 CGCG CG^CG
1536 CGCG
Bsu36I 4153 CCTTAGG CC^T[ACGT]AGG
4466 CCTCAGG
DsaI 2596 CCATGG C^C[GA][CT]GG
5119 CCACGG
Eco47III 1468 AGCGCT AGC^GCT
4424 AGCGCT
Eco81I 4153 CCTTAGG CC^T[ACGT]AGG
4466 CCTCAGG
EcoT22I 952 ATGCAT ATGCA^T
3845 ATGCAT
HaeII 1468 AGCGCT [GA]GCGC^[CT]
4424 AGCGCT
HindIII 5162 AAGCTT A^AGCTT
5359 AAGCTT
MamI 1551 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4457 GATCCTTATC
MroI 2109 TCCGGA T^CCGGA
3076 TCCGGA
MvnI 107 CGCG CG^CG
1536 CGCG
NsiI 952 ATGCAT ATGCA^T
3845 ATGCAT
PflMI 267 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4421 CCAAGCGCTGG
RcaI 1477 TCATGA T^CATGA
5211 TCATGA
SacI 3049 GAGCTC GAGCT^C
4329 GAGCTC
ScaI 2641 AGTACT AGT^ACT
3914 AGTACT
Van91I 267 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4421 CCAAGCGCTGG
XbaI 723 TCTAGA T^CTAGA
3306 TCTAGA
XcmI 2863 CCAAACTTTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4638 CCATGATCATACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BalI BanII BbvI
BclI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SpeI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI