HUGE

Gene/Protein Characteristic Table for KIAA1087

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00734
Accession No. :	AB029010
Description :	Sodium/calcium exchanger 2 precursor.
HUGO Gene Name :	solute carrier family 8 (sodium/calcium exchanger), member 2 (SLC8A2)
Clone Name :	hk02378 [Vector Info]
Flexi ORF Clone :	pF1KSDA1087
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 4282 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1423 bp

Genome contig ID gi42406306r_52523735
PolyA signal sequence
(CATAAA,-24)
+----*----+----*----+----*----+----
GTAAAAGTTGCCATAAAACTTTGAAATTCTGCCTG
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AAAAACAGTCCCGTGGGAAGCTGGGATTCAGGAGGAGGGGGCTAATAAGG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 19 r 52623735 52666934 10 99.6 Perfect prediction

Features of the protein sequence	Description

Length: 952 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9UPR5	100.0	Sodium/calcium ...	Homo sapiens
XP_615995	97.8	similar to Sodi...	Bos taurus
EDM08336	97.0	rCG54086 [Rattu...	Rattus norvegicus
P48768	96.7	Sodium/calcium ...	Rattus norvegicus
EDL42108	93.3	solute carrier ...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR004836 784 796 PR01259 Sodium/calcium exchanger protein

IPR004836 797 808 PR01259 Sodium/calcium exchanger protein

IPR004836 810 822 PR01259 Sodium/calcium exchanger protein

IPR004836 823 836 PR01259 Sodium/calcium exchanger protein

HMMPfam IPR004837 115 274 PF01699 Sodium/calcium exchanger membrane region

IPR003644 415 514 PF03160 Na-Ca exchanger/integrin-beta4

IPR003644 543 643 PF03160 Na-Ca exchanger/integrin-beta4

IPR004837 788 941 PF01699 Sodium/calcium exchanger membrane region

HMMSmart IPR003644 415 514 SM00237 Na-Ca exchanger/integrin-beta4

IPR003644 543 643 SM00237 Na-Ca exchanger/integrin-beta4

HMMTigr IPR004836 27 952 TIGR00845 Sodium/calcium exchanger protein

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR004836	784	796	PR01259	Sodium/calcium exchanger protein
IPR004836	797	808	PR01259	Sodium/calcium exchanger protein
IPR004836	810	822	PR01259	Sodium/calcium exchanger protein
IPR004836	823	836	PR01259	Sodium/calcium exchanger protein
HMMPfam	IPR004837	115	274	PF01699	Sodium/calcium exchanger membrane region
IPR003644	415	514	PF03160	Na-Ca exchanger/integrin-beta4
IPR003644	543	643	PF03160	Na-Ca exchanger/integrin-beta4
IPR004837	788	941	PF01699	Sodium/calcium exchanger membrane region
HMMSmart	IPR003644	415	514	SM00237	Na-Ca exchanger/integrin-beta4
IPR003644	543	643	SM00237	Na-Ca exchanger/integrin-beta4
HMMTigr	IPR004836	27	952	TIGR00845	Sodium/calcium exchanger protein

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 29 PPPMAPLALVGVTLLLAAPPCSG 51 SECONDARY 23

2 105 VYFVAMVYMFLGVSIIADRFMAA 127 PRIMARY 23

3 149 VGTVRIWNETVSNLTLMALGSSA 171 SECONDARY 23

4 193 PGTIVGSAAFNMFVVIAVCIYVI 215 PRIMARY 23

5 226 LRVFFVTASWSIFAYVWLYLILA 248 PRIMARY 23

6 256 QVWEALLTLVFFPVCVVFAWMAD 278 PRIMARY 23

7 750 PSCFDYVMHFLTVFWKVLFACVP 772 SECONDARY 23

8 781 ACFGVSILVIGLLTALIGDLASH 803 PRIMARY 23

9 853 NAVNVFLGLGVAWSVAAVYWAVQ 875 PRIMARY 23

10 883 TGTLAFSVTLFTVFAFVGIAVLL 905 PRIMARY 23

11 924 LATTALFLGLWLLYILFASLEAY 946 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	29	PPPMAPLALVGVTLLLAAPPCSG	51	SECONDARY	23
2	105	VYFVAMVYMFLGVSIIADRFMAA	127	PRIMARY	23
3	149	VGTVRIWNETVSNLTLMALGSSA	171	SECONDARY	23
4	193	PGTIVGSAAFNMFVVIAVCIYVI	215	PRIMARY	23
5	226	LRVFFVTASWSIFAYVWLYLILA	248	PRIMARY	23
6	256	QVWEALLTLVFFPVCVVFAWMAD	278	PRIMARY	23
7	750	PSCFDYVMHFLTVFWKVLFACVP	772	SECONDARY	23
8	781	ACFGVSILVIGLLTALIGDLASH	803	PRIMARY	23
9	853	NAVNVFLGLGVAWSVAAVYWAVQ	875	PRIMARY	23
10	883	TGTLAFSVTLFTVFAFVGIAVLL	905	PRIMARY	23
11	924	LATTALFLGLWLLYILFASLEAY	946	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: CAGGTGAAGATAGTTGATGAC

Primer_r
: ATATGACTCCTCGATGATGAC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 19

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: CCAGAGGACCAATGCTACAAG
Primer_r	: GGGCAGGAAACATCAACAGTA
PCR product length	: 176 bp
PCR conditions	: 95 °C15 sec64 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	1423 bp
Genome contig ID	gi42406306r_52523735
PolyA signal sequence (CATAAA,-24)	+--------+--------+----*----+---- GTAAAAGTTGCCATAAAACTTTGAAATTCTGCCTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAAACAGTCCCGTGGGAAGCTGGGATTCAGGAGGAGGGGGCTAATAAGG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	19	r	52623735	52666934	10	99.6	Perfect prediction

Primer_f	: CAGGTGAAGATAGTTGATGAC
Primer_r	: ATATGACTCCTCGATGATGAC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1087

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information