##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AscI AseI AsnI Asp718I BamHI
BciVI BclI BglII BsaWI BseAI
BsiWI BspDI BspEI BspHI BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MfeI MroI
MunI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AccI 331 GTCTAC GT^[AC][GT]AC
AflII 1942 CTTAAG C^TTAAG
Alw44I 1804 GTGCAC G^TGCAC
AlwNI 4220 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3365 CCTCAGG CC^T[ACGT]AGG
ApaLI 1804 GTGCAC G^TGCAC
Asp700I 2565 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4090 CCTAGG C^CTAGG
BbvCI 3733 CCTCAGC CCTCAGC(-5/-2)
BfrI 1942 CTTAAG C^TTAAG
BlnI 4090 CCTAGG C^CTAGG
BsaBI 411 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 4141 GCATTC GAATGC(1/-1)
BsrDI 2700 CATTGC GCAATG(2/0)
BstAPI 3899 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3365 CCTCAGG CC^T[ACGT]AGG
DrdI 1717 GACGTGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3365 CCTCAGG CC^T[ACGT]AGG
MamI 411 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2090 CATATG CA^TATG
PstI 2509 CTGCAG CTGCA^G
PvuII 1090 CAGCTG CAG^CTG
SacI 3372 GAGCTC GAGCT^C
ScaI 2324 AGTACT AGT^ACT
StuI 3993 AGGCCT AGG^CCT
TfiI 3770 GAATC G^A[AT]TC
XmnI 2565 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 3134 AAATTT [GA]^AATT[CT]
4271 AAATTC
AhdI 274 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3864 GACCGAGGGTC
ApoI 3134 AAATTT [GA]^AATT[CT]
4271 AAATTC
AspEI 274 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3864 GACCGAGGGTC
AspI 471 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2434 GACTCTGTC
BalI 323 TGGCCA TGG^CCA
1625 TGGCCA
BbrPI 1341 CACGTG CAC^GTG
1690 CACGTG
BcgI 1277 GCATCTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3845 GCAGGACCCTCG
Bsp1407I 860 TGTACA T^GTACA
2807 TGTACA
BspLU11I 335 ACATGT A^CATGT
608 ACATGT
BsrGI 860 TGTACA T^GTACA
2807 TGTACA
BstXI 2492 CCAGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3101 CCATCCTGTTGG
EagI 1235 CGGCCG C^GGCCG
2725 CGGCCG
Eam1105I 274 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3864 GACCGAGGGTC
EclXI 1235 CGGCCG C^GGCCG
2725 CGGCCG
Eco52I 1235 CGGCCG C^GGCCG
2725 CGGCCG
MluI 1817 ACGCGT A^CGCGT
2528 ACGCGT
MluNI 323 TGGCCA TGG^CCA
1625 TGGCCA
MscI 323 TGGCCA TGG^CCA
1625 TGGCCA
NcoI 92 CCATGG C^CATGG
326 CCATGG
PflFI 471 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2434 GACTCTGTC
PmaCI 1341 CACGTG CAC^GTG
1690 CACGTG
PmlI 1341 CACGTG CAC^GTG
1690 CACGTG
PshAI 786 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2926 GACCCTGGTC
SfcI 66 CTGTAG C^T[GA][CT]AG
2509 CTGCAG
SspBI 860 TGTACA T^GTACA
2807 TGTACA
Tth111I 471 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2434 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI Aor51HI ApaI AspHI
AvaI AvaII AviII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssHII BssKI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfoI SmaI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI