##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccI AclI Alw44I ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BbrPI BcgI
BciVI BlnI BlpI Bpu1102I BsaAI
BsiWI BspDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraIII EcoO65I EcoT22I
FseI FspI HpaI KpnI MluI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 4051 GACGTC GACGT^C
AccIII 3483 TCCGGA T^CCGGA
AflIII 3954 ACATGT A^C[GA][CT]GT
AgeI 1326 ACCGGT A^CCGGT
AhdI 314 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 3577 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 314 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2942 GAGCTC G[AT]GC[AT]^C
BalI 3256 TGGCCA TGG^CCA
BamHI 3393 GGATCC G^GATCC
BbvCI 914 CCTCAGC CCTCAGC(-5/-2)
BclI 3975 TGATCA T^GATCA
BglII 651 AGATCT A^GATCT
BsaBI 3678 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3483 TCCGGA T^CCGGA
BseRI 2927 CTCCTC GAGGAG(10/8)
BsiHKAI 2942 GAGCTC G[AT]GC[AT]^C
Bsp1407I 4186 TGTACA T^GTACA
BspEI 3483 TCCGGA T^CCGGA
BspLU11I 3954 ACATGT A^CATGT
BsrGI 4186 TGTACA T^GTACA
BstAPI 4018 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 3680 TTTAAA TTT^AAA
Eam1105I 314 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1473 GAATTC G^AATTC
EcoRV 456 GATATC GAT^ATC
FbaI 3975 TGATCA T^GATCA
HincII 2039 GTTGAC GT[CT]^[GA]AC
HindII 2039 GTTGAC GT[CT]^[GA]AC
HindIII 3236 AAGCTT A^AGCTT
MamI 3678 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3256 TGGCCA TGG^CCA
MroI 3483 TCCGGA T^CCGGA
MscI 3256 TGGCCA TGG^CCA
PinAI 1326 ACCGGT A^CCGGT
PpuMI 444 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 2942 GAGCTC GAGCT^C
SapI 3694 GCTCTTC GCTCTTC(1/4)
SmaI 158 CCCGGG CCC^GGG
SspBI 4186 TGTACA T^GTACA
SwaI 3679 ATTTAAAT ATTT^AAAT
XmaCI 158 CCCGGG C^CCGGG
XmaI 158 CCCGGG C^CCGGG
XmnI 3577 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 59 CGCG CG^CG
107 CGCG
AcsI 403 AAATTT [GA]^AATT[CT]
1473 GAATTC
AflII 1604 CTTAAG C^TTAAG
4154 CTTAAG
AlwNI 1172 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
3872 CAGATGCTG
Aor51HI 783 AGCGCT AGC^GCT
2964 AGCGCT
ApoI 403 AAATTT [GA]^AATT[CT]
1473 GAATTC
BfrI 1604 CTTAAG C^TTAAG
4154 CTTAAG
BsaWI 1326 ACCGGT [AT]^CCGG[AT]
3483 TCCGGA
BsgI 3948 CTGCAC GTGCAG(16/14)
4165 GTGCAG
BsiEI 64 CGGCCG CG[GA][CT]^CG
195 CGGCCG
BspHI 861 TCATGA T^CATGA
1990 TCATGA
BspMI 1920 ACCTGC ACCTGC(4/8)
2340 ACCTGC
BstUI 59 CGCG CG^CG
107 CGCG
DraII 444 AGGTCCT [GA]G^G[ACGT]CC[CT]
2472 AGGGCCT
DrdI 266 GACTACTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1064 GACTTTCCTGTC
EagI 64 CGGCCG C^GGCCG
195 CGGCCG
EclXI 64 CGGCCG C^GGCCG
195 CGGCCG
Eco47III 783 AGCGCT AGC^GCT
2964 AGCGCT
Eco52I 64 CGGCCG C^GGCCG
195 CGGCCG
EcoO109I 444 AGGTCCT [GA]G^G[ACGT]CC[CT]
2472 AGGGCCT
HgaI 3616 GACGC GACGC(5/10)
2140 GCGTC
KspI 58 CCGCGG CCGC^GG
106 CCGCGG
MfeI 2130 CAATTG C^AATTG
2455 CAATTG
MunI 2130 CAATTG C^AATTG
2455 CAATTG
MvnI 59 CGCG CG^CG
107 CGCG
NcoI 2889 CCATGG C^CATGG
3140 CCATGG
RcaI 861 TCATGA T^CATGA
1990 TCATGA
SacII 58 CCGCGG CCGC^GG
106 CCGCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AocI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EarI Eco81I
EcoNI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII