##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AgeI AhdI AscI
AseI AsnI AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BclI
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI Bsp1407I BspEI BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
CpoI Eam1105I EcoNI EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KspI MamI MluI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 3911 GGTACC G^GTACC
AccII 472 CGCG CG^CG
AcyI 2398 GACGCC G[GA]^CG[CT]C
AflII 1340 CTTAAG C^TTAAG
AflIII 4972 ACATGT A^C[GA][CT]GT
Asp718I 3911 GGTACC G^GTACC
BciVI 4324 GTATCC GTATCC(6/5)
BfrI 1340 CTTAAG C^TTAAG
BglII 1872 AGATCT A^GATCT
BsaHI 2398 GACGCC G[GA]^CG[CT]C
BsmBI 2616 GAGACG CGTCTC(1/5)
BsmI 1372 GAATGC GAATGC(1/-1)
BspDI 3376 ATCGAT AT^CGAT
BspHI 3364 TCATGA T^CATGA
BspLU11I 4972 ACATGT A^CATGT
BsrFI 5112 ACCGGC [GA]^CCGG[CT]
BssSI 5386 CTCGTG CACGAG(-5/-1)
BstUI 472 CGCG CG^CG
Cfr10I 5112 ACCGGC [GA]^CCGG[CT]
ClaI 3376 ATCGAT AT^CGAT
EagI 346 CGGCCG C^GGCCG
EclXI 346 CGGCCG C^GGCCG
Eco52I 346 CGGCCG C^GGCCG
HgaI 2398 GACGC GACGC(5/10)
Hin1I 2398 GACGCC G[GA]^CG[CT]C
HincII 4844 GTCAAC GT[CT]^[GA]AC
HindII 4844 GTCAAC GT[CT]^[GA]AC
KpnI 3911 GGTACC GGTAC^C
MvnI 472 CGCG CG^CG
PaeR7I 2953 CTCGAG C^TCGAG
RcaI 3364 TCATGA T^CATGA
SmaI 4144 CCCGGG CCC^GGG
SpeI 900 ACTAGT A^CTAGT
SspI 78 AATATT AAT^ATT
StuI 2115 AGGCCT AGG^CCT
XhoI 2953 CTCGAG C^TCGAG
XmaCI 4144 CCCGGG C^CCGGG
XmaI 4144 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 768 GTAGAC GT^[AC][GT]AC
1628 GTCTAC
AclI 2742 AACGTT AA^CGTT
4521 AACGTT
Alw44I 1192 GTGCAC G^TGCAC
3817 GTGCAC
ApaLI 1192 GTGCAC G^TGCAC
3817 GTGCAC
BalI 1125 TGGCCA TGG^CCA
4199 TGGCCA
BbsI 234 GAAGAC GAAGAC(2/6)
2420 GTCTTC
BglI 43 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
355 GCCCAATTGGC
BlpI 3668 GCTGAGC GC^T[ACGT]AGC
5049 GCTCAGC
Bpu1102I 3668 GCTGAGC GC^T[ACGT]AGC
5049 GCTCAGC
BpuAI 234 GAAGAC GAAGAC(2/6)
2420 GTCTTC
BsiEI 346 CGGCCG CG[GA][CT]^CG
1965 CGGTCG
CelII 3668 GCTGAGC GC^T[ACGT]AGC
5049 GCTCAGC
DrdI 3036 GACGGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4350 GACCCCTATGTC
EcoRV 3360 GATATC GAT^ATC
4173 GATATC
MfeI 358 CAATTG C^AATTG
2471 CAATTG
MluNI 1125 TGGCCA TGG^CCA
4199 TGGCCA
MscI 1125 TGGCCA TGG^CCA
4199 TGGCCA
MunI 358 CAATTG C^AATTG
2471 CAATTG
NcoI 1357 CCATGG C^CATGG
4314 CCATGG
Psp1406I 2742 AACGTT AA^CGTT
4521 AACGTT
SacI 2238 GAGCTC GAGCT^C
3052 GAGCTC
SapI 875 GAAGAGC GCTCTTC(1/4)
2820 GAAGAGC
SexAI 4478 ACCTGGT A^CC[AT]GGT
5324 ACCTGGT
SphI 538 GCATGC GCATG^C
4864 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI Aor51HI ApaI ApoI
Asp700I AspHI AspI AvaI AvaII
BanI BanII BbvI BcgI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstEII BstF5I
BstNI BstPI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI Eco47III Eco81I EcoO109I EcoO65I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmnI