##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BsaAI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SalI SbfI ScaI SexAI SfuI
SgrAI SmlI SnaBI SphI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Tth111I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 95 GACGTC GACGT^C
AccI 98 GTCTAC GT^[AC][GT]AC
AccII 6 CGCG CG^CG
Aor51HI 945 AGCGCT AGC^GCT
AviII 1759 TGCGCA TGC^GCA
BalI 1236 TGGCCA TGG^CCA
BbeI 135 GGCGCC GGCGC^C
BbvCI 249 GCTGAGG CCTCAGC(-5/-2)
BsaBI 1783 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1227 GGTCTC GGTCTC(1/5)
BsmI 1270 GAATGC GAATGC(1/-1)
BsrBI 605 GAGCGG CCGCTC(-3/-3)
BssSI 442 CTCGTG CACGAG(-5/-1)
BstUI 6 CGCG CG^CG
BstYI 1704 AGATCC [GA]^GATC[CT]
DraI 2064 TTTAAA TTT^AAA
Eco47III 945 AGCGCT AGC^GCT
FspI 1759 TGCGCA TGC^GCA
HgaI 271 GCGTC GACGC(5/10)
KasI 135 GGCGCC G^GCGCC
KspI 5 CCGCGG CCGC^GG
MamI 1783 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1704 AGATCC [GA]^GATC[CT]
MluNI 1236 TGGCCA TGG^CCA
MscI 1236 TGGCCA TGG^CCA
MvnI 6 CGCG CG^CG
NaeI 48 GCCGGC GCC^GGC
NarI 135 GGCGCC GG^CGCC
NgoMIV 48 GCCGGC G^CCGGC
NspI 1557 GCATGT [GA]CATG^[CT]
PpuMI 975 GGGACCC [GA]G^G[AT]CC[CT]
PstI 83 CTGCAG CTGCA^G
SacII 5 CCGCGG CCGC^GG
SapI 2016 GAAGAGC GCTCTTC(1/4)
SfiI 1237 GGCCACCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 135 GGCGCC GGC^GCC
SmaI 343 CCCGGG CCC^GGG
SpeI 592 ACTAGT A^CTAGT
TaqI 810 TCGA T^CGA
TthHB8I 810 TCGA T^CGA
XbaI 308 TCTAGA T^CTAGA
XcmI 1404 CCACTGTATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1704 AGATCC [GA]^GATC[CT]
XmaCI 343 CCCGGG C^CCGGG
XmaI 343 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 660 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1585 CAGACCCTG
ApaI 255 GGGCCC GGGCC^C
1623 GGGCCC
Asp700I 625 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
821 GAAGTCCTTC
AvaI 321 CCCGAG C^[CT]CG[GA]G
343 CCCGGG
BglI 17 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1238 GCCACCTTGGC
BlpI 409 GCTGAGC GC^T[ACGT]AGC
1111 GCTGAGC
Bpu1102I 409 GCTGAGC GC^T[ACGT]AGC
1111 GCTGAGC
BsoBI 321 CCCGAG C^[CT]CG[GA]G
343 CCCGGG
BstXI 613 CCAGACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
663 CCACTGGCATGG
CelII 409 GCTGAGC GC^T[ACGT]AGC
1111 GCTGAGC
MaeII 96 ACGT A^CGT
480 ACGT
MseI 1787 TTAA T^TAA
2065 TTAA
NcoI 570 CCATGG C^CATGG
1695 CCATGG
PleI 1061 GACTC GAGTC(4/5)
1292 GAGTC
TfiI 1159 GAATC G^A[AT]TC
2056 GATTC
Tru9I 1787 TTAA T^TAA
2065 TTAA
XmnI 625 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
821 GAAGTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI